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NEBNext® Ultra™ II FS DNA Library Prep KitNovel Enzymatic Fragmentation System

Do you need a faster, more reliable solution for

DNA fragmentation and library construction?

The NEBNext Ultra II FS DNA Library Prep

Kit meets the dual challenge of generating

high quality next gen sequencing libraries from

ever-decreasing input amounts AND simple

scalability. Enzymatic shearing increases library

yields by reducing DNA damage and sample loss.

Our novel fragmentation reagent is combined

with end repair and dA-tailing reagents, and a

single protocol is used for a wide range of input

amounts (100 pg – 500 ng) and sample types.

You’ll be thrilled to pieces with the result – a reliable,

flexible, high-quality library prep that is fast

and scalable.

You’ll bethrilled to pieces.

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc.For more information, please email us at [email protected] use of these products may require you to obtain additional third party intellectual property rights for certain applications.ILLUMINA® and NEXTERA® are registered trademarks of Illumina, Inc.KAPA™ is a trademark of Kapa Biosystems. COVARIS® is a registered trademark of Covaris, Inc.© Copyright 2017, New England Biolabs, Inc.; all rights reserved.

be INSPIRED

drive DISCOVERY

stay GENUINE

NEBNext Ultra II FS DNA produces the highest yields,from a range of input amounts

Libraries were prepared from Human NA19240 genomic DNA using the input amounts andnumbers of PCR cycles shown. For NEBNext Ultra II FS, a 20-minute fragmentation timewas used. For Kapa HyperPlus libraries, input DNA was cleaned up with 3X beads prior tolibrary construction, as recommended, and a 20-minute fragmentation time was used. Illuminarecommends 50 ng input for Nextera, and not an input range; therefore, only 50 ng was usedin this experiment. “Covaris” libraries were prepared by shearing each input amount in 1X TEBufer to an insert size of ~200 bp using a Covaris instrument, followed by library constructionusing the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645). Error bars indicatestandard deviation for an average of 3–6 replicates performed by 2 independent users.

VisitNEBNextUltraII.com

to request your sample today.

0

20

40

60

80

100

120

140

13

100 pg

10

500 pg

9

1 ng

5

50 ng

4

100 ng

3

500 ng

Library

Yield

(nM)

PCR cycles

DNA input

2017 Winner

Flavio Donato, Ph.D.

Kavli Institute

Norwegian University of

Science and Technology

For research on how neural

networks mature during

development to represent

space in the brain

Eppendorf & Science Prize for Neurobiology

The annual Eppendorf & Science Prize for Neurobiology

is an international award which honors young scientists

for their outstanding contributions to neurobiological

research based on methods of molecular and cell biology.

The winner and finalists are selected by a committee

of independent scientists, chaired by Science’s Senior

Editor, Dr. Peter Stern. To be eligible, you must be

35 years of age or younger.

As the Grand Prize Winner, you could be next to receive

> Prize money of US$25,000

> Publication of your work in Science

> Full support to attend the Prize Ceremony held in

conjunction with the Annual Meeting of the Society

for Neuroscience in the USA

> 10-year AAAS membership and online subscription

to Science

> Complimentary products worth US$1,000

from Eppendorf

> An invitation to visit Eppendorf in Hamburg, Germany

It’s easy to apply! Learn more at:

www.eppendorf.com/prize

Call for EntriesApplication Deadline

June 15, 2018

AAAS®andScience®are

registeredtrademarksoftheAmericanAssociationfortheAdvancementofScience,USA.Eppendorf®andtheEppendorfBrandDesignare

registeredtrademarksofEppendorfAG,Germ

any.

Allrights

reserved,includinggraphicsandim

ages.Copyright©

2018byEppendorfAG.KurstinRoePhotography.

2017年获奖者


卡弗里研究所

挪威科技大学

专注发育期间神经网络成熟如

何构建大脑空间的研究

Eppendorf & Science神经生物学奖

一年一度的 Eppendorf & Science神经生物学奖是一项国

际奖项，授予用分子与细胞生物学方法在神经生物学领域取

得非凡成果的青年科学家。冠军及入围候选人均是由

《Science》杂志高级编辑 Peter Stern博士领衔的独立科

学家所组成的委员会评出。年龄不超过 35岁的科学家可以

申请参选。

您可能就是下一位获奖者并获得：

> $25,000美元奖金

>获奖论文发表在《Science》杂志上

>得以全额资助参与美国神经科学协会年会和颁奖仪式

> 10年 AAAS会员和《Science》电子期刊

>赠送价值 $1,000美元的 Eppendorf产品

>获邀参观 Eppendorf位于德国汉堡的总部

申请非常容易！

要了解更多信息，请登陆：


欢迎参选申请截止日期

2018年 6月 15日




any.

Allrights


ages.Copyright©


2017 Winner


Kavli Institute

Norwegian University of

Science and Technology

For research on how neural

networks mature during

development to represent

space in the brain

Eppendorf & Science for Neurobiology

Eppendorf & Science神経生物学賞は、分子生物学や細胞

生物学に基づく神経生物学研究において、卓越した貢献を

なされた若手科学者に贈呈している国際賞です。最終選

考選出者ならびに受賞者は、Science誌編集主任Dr. Peter

Sternをはじめとする科学者達の独立委員会によって選出

されます。35歳までの研究者が応募条件できます。

As the Grand Prize Winner, you could be next to receive

>賞金：25,000米ドル

> Science誌に研究内容を掲載

>米神経科学学会年次総会に併せて開催される授賞式へ

の参加を全面サポート

> 10年間のAAAS会員資格およびScience誌のオンライン

購読権

> 1,000米ドル相当のエッペンドルフ製品を提供

>ドイツ、ハンブルクのエッペンドルフ本社にご招待

応募は簡単です！詳しくはこちら：


Call for Entries申込期限

2018年6月15日




any.

Allrights


ages.Copyright©


THe 6sHarpestperspectives

for focused science and technologysolutions in life science

PROMOTIONAL FEATURE

The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigmain the U.S. and Canada.

MilliporeSigma, the vibrant M, Milli-Q, Millipore, SAFC, BioReliance, Supelco and Sigma-Aldrich are trademarks ofMerck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners.Detailed information on trademarks is available via publicly accessible resources.

© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Our mission is to collaborate

with the global scientific

community to solve the

toughest problems in life

science. To achieve this, we

have brought together the

world’s leading Life Science

brands to create a world-class

portfolio: Millipore®, SAFC®,

BioReliance®, Sigma-Aldrich®,

Milli-Q® and Supelco®.

A science andtechnology ecosystem

MilliporeSigma has brought together

the world’s leading Life Science brands

to solve the toughest problems in life

science. In particular, you may be

interested in the following brands:

Sigma-Aldrich® continues to develop

its broad portfolio of state-of-the-art

lab and production materials,

paired with technical support and

scientific partnerships.

Milli-Q® offers a continually pioneering

range of intuitive, easy-to-use lab water

instruments that seamlessly integrate

into your daily work.

Millipore® provides proven preparation,

separation, filtration and testing

products and technologies.

Supelco® continues to be a trusted

resource for accurate and reliable

analytical products, developed

by analytical chemists, for

analytical chemists.

Support you can count on

At MilliporeSigma, we offer a world-class

portfolio with over 300,000 high-quality

products and services – from research

to commercial manufacturing.

With 19,000 life science employees

in 66 countries across the globe,

we have 130 distribution centers

and 10 global customer collaboration

facilities across North America, Europe,

Asia and Latin America.

Customer-centricinnovation

We are dedicated to putting our

customers first, and by collaborating

we can help advance life science.

Together, we can improve and

expand global access to health with

unparallelled services and support

under one banner.

Seamless access

With 24/7 customer support, an

efficient ordering and delivery service,

and an unmatched e-commerce

platform, you get exactly what you

need, when you need it.

A sharper look

As part of the exciting life science

restructure, we are refreshing our existing

packaging design and labeling to better

serve you and reflect the vibrant nature

of our business and vision.

Let us help you

Find out how we collaborate to

solve your toughest problems

at SigmaAldrich.com/

advancinglifescience

#howwesolve

The life science business of Merck operates as MilliporeSigma in the U.S. and Canada.

Merck, the vibrant M, Milli-Q, Millipore, SAFC, BioReliance, Supelco and Sigma-Aldrich are trademarks ofMerck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners.Detailed information on trademarks is available via publicly accessible resources.

© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Our mission is to collaborate

with the global scientific

community to solve the

toughest problems in life

science. To achieve this, we

have brought together the

world’s leading Life Science

brands to create a world-class

portfolio: Millipore®, SAFC®,

BioReliance®, Sigma-Aldrich®,

Milli-Q® and Supelco®.

A science andtechnology ecosystem

Merck has brought together the world’s

leading Life Science brands to solve the

toughest problems in life science.

In particular, you may be interested in

the following brands:

Sigma-Aldrich® continues to develop

its broad portfolio of state-of-the-art

lab and production materials,

paired with technical support and

scientific partnerships.

Milli-Q® offers a continually pioneering

range of intuitive, easy-to-use lab water

instruments that seamlessly integrate

into your daily work.

Millipore® provides proven preparation,

separation, filtration and testing

products and technologies.

Supelco® continues to be a trusted

resource for accurate and reliable

analytical products, developed

by analytical chemists, for

analytical chemists.

Support you can count on

At Merck, we offer a world-class

portfolio with over 300,000 high-quality

products and services – from research

to commercial manufacturing.

With 19,000 life science employees

in 66 countries across the globe,

we have 130 distribution centres

and 10 global customer collaboration

facilities across North America, Europe,

Asia and Latin America.

Customer-centricinnovation

We are dedicated to putting our

customers first, and by collaborating

we can help advance life science.

Together, we can improve and

expand global access to health with

unparallelled services and support

under one banner.

Seamless access

With 24/7 customer support, an

efficient ordering and delivery service,

and an unmatched e-commerce

platform, you get exactly what you

need, when you need it.

A sharper look

As part of the exciting life science

restructure, we are refreshing our existing

packaging design and labelling to better

serve you and reflect the vibrant nature

of our business and vision.

Let us help you

Find out how we collaborate to

solve your toughest problems

at SigmaAldrich.com/

advancinglifescience

#howwesolve

THe 6sHarpestperspectives

for focused science and technologysolutions in life science

PROMOTIONAL FEATURE

Expert Pharma &Biopharma Manufacturing& Testing Services

Tailored Pharma& Biopharma RawMaterial Solutions

Proven Preparation,Separation, Filtration& Testing Products

State-of-the-Art Lab &Production Materials

Pioneering LabWater Solutions

Trusted AnalyticalProducts

6 powerfulpropellers

to accelerate expert scienceand technology solutions inlife science

MilliporeSigma has brought together the world‘sleading Life Science brands, so whatever your lifescience problem, you can benefit from our expert

products and services.

To fnd out how MilliporeSigma can help you work,visit SigmaAldrich.com/advancinglifescience

#howwesolve

The life science business of Merck KGaA,Darmstadt, Germany operates as

MilliporeSigma in the U.S. and Canada.

MilliporeSigma, the vibrant M, Milli-Q, Millipore, SAFC, BioReliance,Supelco and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt,Germany or its affiliates. All other trademarks are the property of theirrespective owners. Detailed information on trademarks is available viapublicly accessible resources.

© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates.All Rights Reserved.

Expert Pharma &Biopharma Manufacturing& Testing Services

Tailored Pharma& Biopharma RawMaterial Solutions

Proven Preparation,Separation, Filtration& Testing Products

State-of-the-Art Lab &Production Materials

Pioneering LabWater Solutions

Trusted AnalyticalProducts

Merck has brought together the world‘s leadingLife Science brands, so whatever your life scienceproblem, you can benefit from our expert products

and services.

To fnd out how the life science businessof Merck can help you work, visitSigmaAldrich.com/advancinglifescience

#howwesolve

The life science business of Merck operatesas MilliporeSigma in the U.S. and Canada.

Merck, the vibrant M, Milli-Q, Millipore, SAFC, BioReliance, Supelcoand Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt,Germany or its affiliates. All other trademarks are the propertyof their respective owners. Detailed information on trademarks isavailable via publicly accessible resources.

© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates.All Rights Reserved.

6 powerfulpropellers

to accelerate expert scienceand technology solutions inlife science

342 20 APRIL 2018 • VOL 360 ISSUE 6386 sciencemag.org/custom-publishing SCIENCE

new products: microscopy/imaging

LIFE SCIENCE TECHNOLOGIES Produced by the Science/AAAS Custom Publishing Office

Tissue Imaging System

The Vectra Polaris Automated

Quantitative Pathology Imaging

System integrates both multi-

spectral imaging and automated

slide scanning to better visual-

ize, analyze, quantify, and phe-

notype immune cells in situ in

FFPE tissue sections and tissue

microarrays (TMAs). The fully automated system provides research-

ers unparalleled speed, performance, and versatility for extracting

proteomic and morphometric information from intact FFPE tissue sec-

tions and TMAs. Vectra Polaris is the only instrument on the market

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inForm image analysis software, its multiplexing capability enables

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better, faster decisions.

PerkinElmer

For info: 800-762-4000

www.perkinelmer.com/cancer-immunology

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this

space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not

implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/about/new-products-section for more information.

Microscope Camera

The Lumenera INFINITY3-6UR is an ideal general-purpose camera for

most microscopy applications due to its 6-MP resolution, excellent color

reproduction, and speed, with the light sensitivity needed for low-light

applications. Built on Sonyís EXview HAD II sensor technology, this

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and very low noise. With its 2x2 binning, there is a fourfold increase in

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industrial applications requiring optimal color reproduction, extreme

sensitivity, increased resolution, and high speed.

Lumenera

For info: 613-736-4077

www.lumenera.com

Non-Browning Zoom Lens

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specialist non-browning glasses, it produces clear, sharp images

without the strong yellow tint that has traditionally been a limiting

issue when using radiation-resistant lenses on color sensors. It is

designed to operate in environments subject to high-level radiation,

such as nuclear fuel handling sites, reactor active zones, and nuclear

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resolution and minimum geometric distortion from 400 nm to

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add-on adapters.

Resolve Optics

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www.resolveoptics.com

Enhanced Confocal Detector

The ZEISS Airyscan is an area detector. Unlike traditional confocal

microscopes, which reject photons from outside of the focal plane

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sensitivity, superresolution, and high acquisition speeds. The new

2D Superresolution mode takes advantage of the fact that Airyscan

captures information from the x, y, and z planes with the confocal

point spread function. A new exclusive processing algorithm uses this

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distinguishing between photons originating from the focal plane of

0.2 a.u. and photons from outside of this focal plane. In a traditional

confocal microscope, a researcher could only close the pinhole to

0.2 a.u. to attempt the same optical sectioning. This would mean

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signal-to-noise drastically.

ZEISS

For info: 800-233-2343

www.zeiss.com

Biomolecular Imager

The Amersham Typhoon NIR Plus is a modular, upgradeable near-

infrared (NIR) imaging system from GE Healthcare Life Sciences. Its

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as well as a third visible laser channel for triplex protein detection,

to facilitate normalization and the reduction of blot-to-blot variation.

Ideal for multiuser environments, it delivers extremely sensitive

detection, high image resolution, and a broad linear dynamic range with

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plates simultaneously, it is also suitable for high-throughput screening.

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compatible with most commercially available NIR detection reagents.

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magnitude, and sensitivity as low as 3 picograms, it accommodates a

wide range of samples, including tissue sections, plant leaves, and cell

colonies, in addition to traditional gels, membranes, multiwell plates,

and dishes.

GE Healthcare Life Sciences

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bioresearch.gehealthcare.info

Biological Microscopes

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during long periods of routine microscopy observations. In both

instruments, the stage is 70 mm lower than in the previous model,

resulting in a large workspace under the eyepiece that facilitates

checking or swapping samples with just one hand. In addition, the stage

and coaxial focus knobs have been lowered, so users can reach them

while their forearms remain on the desk. The low-positioned revolving

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movement. To improve durability and reduce running costs, we have

added a centering-free LED light source with a long 60,000-h lifetime,

providing uniform illumination and constant color temperature at any

brightness level. This means users get natural color representation

from a wide range of stains and can perform analyses faster and more

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Olympus

For info: 704-877-8801

www.olympus-lifescience.com/en/microscopes/upright

The Alexander von Humboldt Foundation is now calling for

applications for the Sofja Kovalevskaja Award, one of the most

valuable academic awards in Germany. With funding of up to

€1.65 million, it creates unique opportunities for excellent

researchers to conduct their own research in Germany: award

winners are invited to spend five years working at the institute

of their choice and setting up their own research groups –

independently and virtually free of bureaucracy.

Scientists and scholars of all disciplines from abroad who have

completed their doctorates within the last six years are eligible

to apply. The award targets outstanding talent and a creative

approach to research.

The Humboldt Foundation actively promotes equal opportu-

nities and therefore particularly welcomes applications from

women researchers.

The closing date for applications is 31 July 2018. More information: www.humboldt-foundation.de/SKP_en

Sofja Kovalevskaja Award – Become a researchgroup leader in Germany

€1.65 million for young researchers from all disciplines

Alexander von Humboldt Foundation

Jean-Paul-Str. 12

53173 Bonn

Germany

[email protected]

searchmore independence + more opportunities + more scope

Exzellenz verbindet –

be part of a worldwide network.

www.humboldt-foundation.de

you’ll be thrilled to pieces.€¦ · brands to create a world-class portfolio: millipore®,...

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