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Research Center for Zoonosis Control
Hokkaido University
History of Research Center
for Zoonosis Control 2014 ・Global Institution for Collaborative Research and Education (MEXT)
2012 • Science and Technology Research Partnership for Sustainable Development (JICA/JST)
2011 • Collaborating Centre for Zoonoses Control (WHO)
• Program for Leading Graduate Schools (JSPS)
2010 • Joint Usage / Research Center (MEXT)
• Leading-edge Research Infrastructure Program (JSPS)
• Japan Initiative for Global Research Network on Infectious Diseases (MEXT)
2009 • Strategic Basic Research Programs (JST)
2008 • Global COE program (JSPS)
• Science and Technology Research Partnership for Sustainable Development (JICA/JST)
2005 • Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases (MEXT)
• Research Network for the Control of Infectious Diseases (MEXT)
• Establishment of “Research Center for Zoonosis Control” (MEXT)
2004 • World Reference Laboratory for Avian Influenza (OIE)
2003 • The 21st Century COE program (JSPS)
• Presentation at the council on “Prevention and Control of Zoonoses-proposal of a national project”,
“Committee for Zoonosis” Council for Science and Technology Policy, (Cabinet Office)
1996 • Special Group Training Course in Diagnosis and Control of Rabies and Other Viral Zoonoses (JICA) -2000
WHO Collaborating Centre for Zoonoses Control, JPN-87
In 2010 Dec, Hiroshi Kida hosted the Regional
Workshop on Collaboration between Human and
Animal Health Sectors on Zoonoses Prevention and
Control, in which the members from World Health
Organization (WHO), Food and Agriculture
Organization of the United Nations (FAO), and World
Organisation for Animal Health (OIE) got together for
the first time in the world.
In 2011 Nov, WHO designated the Hokkaido
University Research Center for Zoonosis Control as
the “WHO Collaborating Centre for Zoonoses Control”,
and Professor Kida became Head of the Center. The
inauguration ceremony and “the Regional Forum of
Collaborating/ Reference Centres on Emerging
Infectious Diseases and Zoonoses” was held in 2011
Dec.
Key activities and achievements in Research Centre for Zoonosis Control from 2012 to 2014
Since 2005, the Research Center for Zoonosis Control
had invited oversee researchers and risk managers,
and organized several training courses; training courses
of ecology, pathogenicity, and molecular biology of
influenza virus, filovirus, and rabies viruses, training
course for tuberculosis and influenza diagnosis
responding to a request from WHO. General zoonosis
and individual training courses with young researchers
were supported by Japan International Cooperation
Agency and other education funds. Adding to that, we
organized training course of genetic diagnosis in
leptospirosis in Philippines and of bioinformatics for
studies on zoonoses in Thailand.
The Research Center for Zoonosis Control has conducted
the global surveillance of influenza and tuberculosis and
released data through internet or scientific peer reviewed
paper. Pathogenicity, antigenicity, genetic information, and
yield in chicken embryo of influenza viruses have been
analyzed, databased and opened for Web site. In 2010,
isolation of H5N1 HPAI viruses through the surveillance
contributed to preparedness for subsequent HPAI
outbreaks in Japan.
Clinical isolates of Mycobacterium tuberculosis complex
from humans, monkey, cattle, deer, lechwe and elephants
from Zambia, Nepal, Bangladesh, Myanmar, the
Philippines and Thailand were collected in collaboration
with local researchers. These samples were analyzed
genotype and drug resistance-associated genes of the
strains.
The Research Center for Zoonosis Control was
registered into the WHO Global Outbreak Alert Network
(GOARN) and continued capacity buildings to provide
technical, material, and human resource to the outbreak
area in responding to emergency request.
Responding to urgent requests from Zambia
Government upon the outbreak of Ebola virus infection
in West Africa, 2014, we dispatched an expert of Ebola
virus for capacity building for Ebola virus infection
including establishment of emerging response guideline
for suspected case in Points of Entry and provision of
technical and material resources for diagnosis of Ebola
virus to University of Zambia
Responding to requests from Zambia Government upon
the outbreak of human anthrax followed by massive
deaths of wildlife along Zambezi Valley in 2012, we
dispatched experts of bacteriology and molecular
biology for follow-up studies to monitor environment
(soil and water) of the past epidemic sites and advise
the government on procedures of decontamination.
Prof Kida was invited as the Temporary Advisor to Asia-
Pacific Workshop on Multi-sectoral Collaboration for
Zoonoses Prevention and Control, 27-29, Kathmandu
Nepal. His expertise regarding control for emerging
infectious disease in Asia Pacific Region and zoonotic
influenza control was shared with a 120 participants from
public and animal health officials in Asia Pacific Region.
The Research Center for Zoonosis Control seconded one
staff to ESR/DSE/WPRO to contribute to provide the
technical expertise and facilitating the risk assessment for
zoonotic events in the WPRO.
Public Health Agency of Canada (WHO Collaborating Centre for Emerging and Zoonotic Diseases Detection,
Diagnostics, Reference and Research, Winnipeg, Canada, CAN-22) on Ebola virus
Nagasaki University (WHO Collaborating Centre for Reference and Research on Tropical and Emerging Virus
Diseases, Nagasaki, Japan, JPN-67) on Ebola virus
National Institute of Infectious Diseases (WHO Collaborating Centre for Reference and Research on Influenza,
Tokyo, Japan, JPN-64) on influenza.
The University of Melbourne, Department of Microbiology and Immunology
( WHO Collaborating Centre for Reference and Research on Influenza) on influenza
Research Institute of Tuberculosis (RIT) (WHO Collaborating Centre for Reference, Research and Training on
Tuberculosis) on tuberculosis.
Training on zoonoses and capacity
building for Member States
Data collection and dissemination of
information related to zoonoses
Collaboration or interaction with other WHO CC in the context of the ongoing
activities to zoonoses control
Isolates from water birds (69 combinations)
Reassortants generated in the lab (75 combinations)
Activities for establishment/improvement/maintenance of
Biobank of influenza virus strains have been continued
and their genetic information that will be used as
references for the diagnosis, surveillance, and vaccine
preparation. Influenza virus isolates of 75 combinations of
the HA and NA subtypes have been isolated from fecal
samples of ducks in surveillance studies. So far, 69 other
combinations have been generated by the genetic
reassortment procedure in chicken embryos. Thus, 2,000
non-pathogenic avian influenza viruses of all 144
combinations of the HA and NA subtypes have been
stocked. The database is open for sharing the genetic
information and biological materials for research and
development of vaccines and diagnostic use for the control
of animal and human influenza.
(http://virusdb.czc.hokudai.ac.jp/index.html)
Enhancement of surveillance and laboratory diagnosis for priority zoonoses and
application for disease control
Support Member States in developing
and implementing zoonoses control in
line with APSED Support WHO in Member States in
outbreak investigation on potential
zoonoses
Advanced and Comprehensive
Studies on Zoonosis Control
Advanced lecture on One Health
with invited speakers from US CDC
and FAO.
In 2012, Core Curriculum for
Zoonosis Control were held
with collaborations of JICA.
A total of 26 trainees from
20 countries joined the
curriculum and not only
Japanese researchers but
also WHO and OIE were
invited as trainers.
To facilitate the rapid diagnosis at the outbreak point in the field, antigen detection diagnosis kits based on Loop-
Medicated Isothermal Amplification (LAMP) methods were developed to diagnose avian influenza virus infection with
subtype H5 and H7N9, tuberculosis, leptospirosis, and African trypanosomiasis and introduced into the field in endemic
areas. The PCR methods for diagnosis of anthrax and rabies were developed, and introduced into Zambia and
Zimbabwe. Ebola virus antigen detection assay using monoclonal antibodies to the Ebola virus and ELISA system to
detect anti-filovirus antibodies were developed to diagnose the filovirus infection.
Development and application of diagnostic tools for Member States
Developed method
Marburgvirus Ebolavirus
existing method
Improvement of filovirus detection
The diagnosis method to detect the RNA of
Ebolavirus and Marburgvirus was developed.
It was revealed that this method is available
for diagnosis using blood serum samples.
Comparison of nested PCR and RT-
LAMP for rabies virus detection
The LAMP kit for tuberculosis was developed
and were introduced into Zambia
Contribution of Hokkaido University Research
Center for Zoonosis Control in control of zoonoses
Anti-microbial resistant
tuberculosis
(China)
Avian influenza
(Mongolia)
Rabies, Tuberculosis
(Sri Lanka)
Tuberculosis
(Nepal, Bangladesh,
Myanmar)
Surveillance and Research collaboration for
hemorrhagic fever, tuberculosis, AMR, and
capacity building for leptospirosis
(Thailand, Vietnam, Philippines, Indonesia)
Global surveillance for emerging
diseases in Hokudai Center for
Zoonosis Control in Zambia
(Zambia)
WHO Western Pacific Regional Office
(Philippines)
OIE-FAO
OIE-FAO Reference Laboratories for Avian Influenza (designated in Jun., 2004)
WHO Collaborating Centre for Zoonoses Control (designated in Nov., 2011)
WHO
Avian influenza
(Russia)
Protozoan
diseases
(Ghana, Sudan) Collaboration with
Southern African Centre
for Infectious Disease
(SACIDS:five countries)Leprosy
(Mexico)
Research and EducationLaboratory Invitation of Leading Researchers Unit
Research collaboration for
tick-borne infectious diseases
(UK, Netherland Belgium)
Genome analysis
of protozoa
(Malaysia)
Research collaboration for Filo
virus infections
(Canada)
Research collaboration for
tuberculosis and filo virus infections
(South Africa)
Tuberculosis
(Egypt)
Rabies, Anthrax
(Zimbabwe)
Research collaboration
for influenza, filo, and
Phlebo viruses
(USA)
The Research Center for Zoonosis Control has established the global network for research collaboration, capacity
development, and education support. We established the Hokudai Center for Zoonosis Control in Zambia as a
research base contributing to clarify the ecology of zoonoses by intensive surveillance in animal populations, and
technical and educational inputs for zoonoses control not only in Zambia but also in African countries. Our
contribution is not limited into the Asian countries but also worldwide, including African countries.