- +
DESCRIPTION
EF reversal. + -. EF. - +. E. A. 360 min. 0 min. B. F. 420 min. 60 min. G. C. 540 min. 180 min. D. H. 660 min. 300 min. Zhang et al., Supplemental Fig. 1 Reversal of field polarity reverse d migration direction. + -. EF. Isolated hiPS cells. - PowerPoint PPT PresentationTRANSCRIPT
- +
Zhang et al., Supplemental Fig. 1
Reversal of field polarity reversed migration direction.
0 min
60 min
180 min
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+ -EF reversal
360 min
420 min
540 min
660 min
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EF
Zhang et al., Supplemental Fig. 2
Electrotaxis of an Isolated and a large colony of hiPS cells.
+ - EFIsolated hiPS cells big colony of hiPS cells
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EF
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xle
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t (u
m)
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* * * *
Zhang et al., Supplemental Fig. 3
Displacement along X axis.
SSEA-4
Oct-4
merger
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B
C
Zhang et al. Supplemental Fig. 4.
Small electric fields did not alter expression of stem cell markers.
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DNO EF NO EF + Y- 27632
+ - + -
EF+ Y-27632EF
Zhang et al., supplemental Fig. 5
ROCK inhibition altered cell morphology and induce protrusions in hiPS cells.
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No EF With EF
Traj
ecto
ry S
peed
(µm
/min
)
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No EF With EF
Dire
cted
ness
Zhang et al. Supplemental Fig. 6
EFs guide anodal migration of dermal fibroblasts in 2D. A, representative images showing cells migrate anodally when treated with EFs (right panel), as compared to non-EFs treated controls (left panel); B and C, quantitative
analysis showing significantly increased directedness (B) and trajectory speed(C) of cells by EFs stimulation. *, P<0.01.
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ecto
ry S
peed
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cted
ness
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Zhang et al. Supplemental Fig. 7
EFs guide anodal migration of dermal fibroblasts in 3D. A, representative images showing cells migrate anodally in 3D when treated with EFs (right panel), as compared to non-EFs treated controls (left panel); B and C,
quantitative analysis showing significantly increased directedness (B) and trajectory speed (C) of cells in 3D by EFs stimulation. *, P<0.01.
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*
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No EF 50mV/mm 100mV/mm
Tra
ject
ory
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eed
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m/M
in)
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ecte
dnes
s
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**
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*
Zhang et al. Supplemental Fig. 8
Galvanotaxis of hiPS cells in 3D at 50mV/mm and 100mV/mm. A, representative images showing anodal migration of hiPS cells at 50mV/mm (left panel) and 100 mV/mm (right panel); B and C, quantitative analysis showing the directedness (B) and migration speed (C) of hiPS cells at indicated EFsstrength. *, P<0.01.