Download - - +
- +
Zhang et al., Supplemental Fig. 1
Reversal of field polarity reversed migration direction.
0 min
60 min
180 min
300 min
+ -EF reversal
360 min
420 min
540 min
660 min
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EF
Zhang et al., Supplemental Fig. 2
Electrotaxis of an Isolated and a large colony of hiPS cells.
+ - EFIsolated hiPS cells big colony of hiPS cells
H
EF
D
90 min
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180 min
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xle
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m)
**
* * * *
Zhang et al., Supplemental Fig. 3
Displacement along X axis.
SSEA-4
Oct-4
merger
A
B
C
Zhang et al. Supplemental Fig. 4.
Small electric fields did not alter expression of stem cell markers.
A
B
C
DNO EF NO EF + Y- 27632
+ - + -
EF+ Y-27632EF
Zhang et al., supplemental Fig. 5
ROCK inhibition altered cell morphology and induce protrusions in hiPS cells.
0
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No EF With EF
Traj
ecto
ry S
peed
(µm
/min
)
-1
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No EF With EF
Dire
cted
ness
Zhang et al. Supplemental Fig. 6
EFs guide anodal migration of dermal fibroblasts in 2D. A, representative images showing cells migrate anodally when treated with EFs (right panel), as compared to non-EFs treated controls (left panel); B and C, quantitative
analysis showing significantly increased directedness (B) and trajectory speed(C) of cells by EFs stimulation. *, P<0.01.
0 h
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100mV/mm
*
*
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No EF With EF
Traj
ecto
ry S
peed
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Dire
cted
ness
NO EF EF + -A200mV/mm
0 h
6 h
0 h
6 h
1 2
1
2
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1
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B C
Zhang et al. Supplemental Fig. 7
EFs guide anodal migration of dermal fibroblasts in 3D. A, representative images showing cells migrate anodally in 3D when treated with EFs (right panel), as compared to non-EFs treated controls (left panel); B and C,
quantitative analysis showing significantly increased directedness (B) and trajectory speed (C) of cells in 3D by EFs stimulation. *, P<0.01.
*
*
0
0.1
0.2
No EF 50mV/mm 100mV/mm
Tra
ject
ory
sp
eed
(µ
m/M
in)
-1.05
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-0.65
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No EF 50mV/mm 100mV/mm
Dir
ecte
dnes
s
0 h
6 h
3 h
50mV/mm 100mV/mm
A
B C
**
*
*
Zhang et al. Supplemental Fig. 8
Galvanotaxis of hiPS cells in 3D at 50mV/mm and 100mV/mm. A, representative images showing anodal migration of hiPS cells at 50mV/mm (left panel) and 100 mV/mm (right panel); B and C, quantitative analysis showing the directedness (B) and migration speed (C) of hiPS cells at indicated EFsstrength. *, P<0.01.
