1% agarose gel dna electrophoresis running time : 52 minutes distance measured from the well to the...
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1% Agarose Gel DNA ElectrophoresisRunning time : 52 minutes
Distance measured from the well to the top of the second band: 4,5 cm
Running voltage: 100 Volt
Solidification time: 14 minutes
Composition: 250 mikroL buffer TAE + 2,5 gram Agarose + 20 mikroL Ethidium Bromide
1 Kb 100 Kb
Second Band
Scale in Cm
2% Agarose Gel DNA ElectrophoresisRunning time : 52 minutesDistance measured from the well to the top of the second band: 3.4 cm
Total Running time: 62 minutesTotal distance measured: 4.5 cm
Running voltage: 100 Volt
Solidification time: 21 minutes
Composition: 500 mL buffer TAE + 10 gram Agarose + 40 mikroL Ethidium Bromide
1 Kb 100 Kb
Second Band
Scale in Cm
3% Agarose Gel DNA ElectrophoresisRunning time : 52 minutesDistance measured from the well to the top of the second band: 2.9 cm
Total Running time: 68 minutesTotal distance measured: 4.5 cm
Running voltage: 100 Volt
Solidification time: 17 minutes
Composition: 250 mikroL buffer TAE + 7.5 gram Agarose + 40 mikroL Ethidium Bromide
1 Kb 100 Kb
Second Band
Scale in Cm
Overall comparisonCross sections of 3 different concentration ofDNA Agarose Gelare illuminated under UV light
1% 2% 3%
Overall Comparison1% 2% 3%
1 kb 1 kb 1 kb100 bp100 bp 100 bp
Increasing the concentration of Agarose gel decreases the electrpphoretic mobility of DNA
Larger Fragments are better resolved in the low agarose concentration while higher concentration allow resolution of smaller DNAs
Comparison5 mikroL of 1 Kb DNA Ladder in 1%,2%
and 3% Agarose Gel Electrophoresis1% 2% 3%
Wells level
3,0 kb
3,0 kb
2,0 kb
2,0 kb
Comparison5 mikroL of 100 bp DNA Ladder in 1%,2% and 3%
Agarose Gel Electrophoresis1% 2% 3%
Wells level
1000 bp
1000 bp
500 bp
500 bp