1 characterization of cell-based adhesive biointerfaces september 11 (mon) and 14 (thurs) dr. david...
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![Page 1: 1 Characterization of Cell-Based Adhesive Biointerfaces September 11 (Mon) and 14 (Thurs) Dr. David Shreiber Dept. of Biomedical Engineering Prof. Moghe](https://reader036.vdocument.in/reader036/viewer/2022062423/56649d785503460f94a5aa1d/html5/thumbnails/1.jpg)
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Characterization of Cell-Based Adhesive Biointerfaces
September 11 (Mon) and 14 (Thurs)Dr. David Shreiber
Dept. of Biomedical Engineering
Prof. Moghe
125:583 Biointerfacial Characterization
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SIGNAL TRANSDUCTIONGENERAL CONTROL MECHANISM
Stimulus
Sensor
Signal Transducer
Regulator
Effector(Response)
ECMAnother CellForce(Form junctions/adhesion complexes)
IntegrinsCadherinsIg Superfamily of cell adhesion molecules (CAMs)Selectins
ProliferationTissue FormationTractionMigrationApoptosisDifferentiation
Cytoskeletal ElementsKinasesG-Proteins
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CELL ADHESION
• Most cells demonstrate anchorage-dependent growth.
• Adhesion can also affect migration, differentiation, and apoptosis.– Includes adhesion to materials for tissue engineering
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FORCES IN BIOLOGICAL INTERACTIONS
Electrostatic double layer: even though cell and bacterial membranes are negatively charged, and you think they’d repel each other, ions can associate with the cell membranes to form a kind of cloud around it. If two cells share the same cloud, there is measurable adhesion between them.
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FORCES IN BIOLOGICAL INTERACTIONS
Electrostatic double layer: even though cell and bacterial membranes are negatively charged, and you think they’d repel each other, ions can associate with the cell membranes to form a kind of cloud around it. If two cells share the same cloud, there is measurable adhesion between them.
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FORCES IN BIOLOGICAL INTERACTIONS
Of course, cells are not in water, and this force is sensitive to the ionic strength of the extracellular environment in vivo and culture medium in vivo.
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FORCES IN BIOLOGICAL INTERACTIONS
Then are the Van der Waals attractive forces, which are due to interactions between oscillating dipoles of the surface molecules.
These forces are very powerful, but only for a small distance.
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FORCES IN BIOLOGICAL INTERACTIONS
If we combine these forces, we can describe the net forces acting on, essentially, colloidal particles. At least this is what the first people were studying who described this theory.
It’s called DLVO theory, named after people from two research groups: Deryagin and Landau (group one) and Verway and Overbeek (group two).
SUMMARY
1.Most cells and biological surfaces are negatively charged
2.In a fluid environment the surface negative charge attracts a layer of mobile counterions (cations) which is known as the "Electrical Double Layer"
3.As bacteria approach a surface or another cell the electric double layers begin to overlap causing an electrostatic repulsive force
4.As bacteria approach a surface they also experience an attractive force known as van der Waals force
5.The combination of these two forces dictates how a bacterial cell adheres and is described by the DLVO theory
6.The DLVO theory predicts that in a solution of physiological ionic strength bacterial cells will be held about 10nm away from a surface and will be unable to approach closer.
7.The electrical double layer shrinks if the ionic strength of the surrounding fluid is increased which allows cells to get nearer to surfaces. This is the reason why adding salt to a bacteria suspension causes the cells to flocculate
8.The stability of bacteria in suspension is very sensitive to the valence of the counterion, therefore, calcium has a greater effect on bacterial adhesion than sodium.
http://www.ncl.ac.uk/dental/oralbiol/oralenv/tutorials/electrostatic.htm
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THERMODYNAMIC ASPECT OF CELL ADHESION
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TISSUE SORTING
What is the work of adhesion for a single liquid?
Wo/o = Eo/A + Eo/A - Eo/o= Eo/A + Eo/A – 0 = 2Eo/A
Suppose
Wo/w >= Wo/o and Wo/w >= Ww/w
Add these to get
Wo/w >= (Wo/o + Ww/w)/2 or >=Eo/A + EW/A
Recall from previous slide that:
Wo/w = EW/A+ Eo/A - Eo/w
So in this case, in order for
EW/A+ Eo/A - Eo/w >= Eo/A + EW/A,
Eo/w must be <= 0….the liquids are totally miscible
If we change the conditions in blue above, we can get separation or sorting.
O
W
WW
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O OO
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WW
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SORTING:Wo/o>Wo/w>=Ww/w
RANDOM:Wo/w>Wo/o>=Ww/w
SEPARATION:Ww/w>=Wo/o>>Wo/w
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CELL/TISSUE SORTING
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FORCES IN CELL ADHESION
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CELL ADHESION: STRENGTH
From Sundar
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ADHESIVE DYNAMICS
From Sundar
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ADHESIVE DYNAMICS
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ADHESIVE DYNAMICS
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MEASURING ADHESION
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ATOMIC FORCE MICROSCOPY
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SURFACE FORCE APPARATUS
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OPTICAL TWEEZERS(LASER TRAP)
A particle encountering the laser beam will be pushed towards the center of the beam, if the particle's index of refraction is higher than that of the surrounding medium.
In a ray optics picture we realize how light is deflected in the particle, resulting in a gradient force that pushes the particle vertically to the propagation of the laserbeam, towards the largest intensity of light (the middle of the laserbeam).
By focusing the light, the gradient force pushes the particle backwards as well. If this force overcomes the propagation force of the laserbeam, the particle is trapped.
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OPTICAL TRAP
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MICROPIPETTE ASPIRATION
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controlled conditions
MEASURING CELL ADHESION
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HOW CAN YOU MEASURE BULK ADHESION STRENGTH IN VITRO?
• Parallel plate flow chamber
• Cone and plate viscometer
• Rotating cylinder
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SCHEMATICS OF ASSEMBLIES
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CELLS CAN EXERT FORCES ON A SUBSTRATE THROUGH ADHESIONS
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TENSEGRITY AND CELL ADHESION
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FORCES DURING MIGRATION
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TISSUE EQUIVALENTS
• Self-assembled biopolymer networks with entrapped tissue cells of interest.
• Cells exert traction on network resulting in compaction of the network and/or locomotion of the cells.
Fibroblast
Cell traction-induced alignment of network
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ICTA - Methods
Lexan Mold
Medium with defined soluble factors
Coverslip
Stainless Steel Well
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FREELY COMPACTING VS. CONSTRAINEDGELS
Freely CompactingUnstressed
Constrained at EndsStressed
Presence of stress -> network alignment -> cell alignment
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DYNAMIC REGULATION OF ADHESION
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IN WHAT TISSUES DO CELLS REGULARLY EXPERIENCE
MECHANOTRANSDUCTION?
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MECHANOTRANSDUCTION
It’s well known that shear flow affects endothelial cell behavior.
How can you study this mechanotransduction system in a
controlled manner?
This would be the intima later of a vessel, which is lined by a confluent layer of endothelial cells.
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LAMINAR FLOW IN A PIPE
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Anyone know what this flow rate law is commonly called?
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SHEAR FLOW ON ENDOTHELIAL CELLS
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Anyone know what this flow rate law is commonly called?
This defines the shear stress experienced by endothelium as a function of vessel radius and flow rate.
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LAMINAR VS. TURBULENT
•
Re low = laminar
Re high = turbulent
μρVD
=Re
Laminar
Turbulent
• D is on the order of microns• Small reagent volume, Shorter
reaction times• “Low Reynolds number flow”
All mixing is through diffusion
In ‘Microfluidics’ …
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IN VIVO FLOW PREDICTIONS
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MECHANOTRANSDUCTION
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HOW CAN YOU MEASURE BULK ADHESION STRENGTH IN VITRO?
• Parallel plate flow chamber
• Cone and plate viscometer
• Rotating cylinder
![Page 41: 1 Characterization of Cell-Based Adhesive Biointerfaces September 11 (Mon) and 14 (Thurs) Dr. David Shreiber Dept. of Biomedical Engineering Prof. Moghe](https://reader036.vdocument.in/reader036/viewer/2022062423/56649d785503460f94a5aa1d/html5/thumbnails/41.jpg)
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FLOW AFFECTS CELL SHAPE
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RESPONSE OF ENDOTHELIAL CELLS TO SHEAR
Just about every aspect of endothelial cell behavior can be regulated in part by mechanical signals.
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RESPONSE OF ENDOTHELIAL CELLS TO SHEAR
Just about every aspect of endothelial cell behavior can be regulated in part by mechanical signals.
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MECHANOTRANSDUCTION IN VITRO
eME
bubble
medium
stir bar
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ECMCELLS
• Cells adhere to matrix by specific receptors that, just by binding, can initiate specific signal cascades
• Forces exerted on ECM are transduced to the cells, and forces by cells can remodel the matrix
• The distribution and strength of adhesion sites (either on cells or matrix/biomaterials) can control cell growth, differentiation, migration, traction etc…
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CELL ADHESION AND BIOMATERIAL DESIGN
• Obviously the type and number of cell adhesion sites can be critical in optimizing a tissue engineered product
• Biomaterials can, therefore, be more than just “biocompatible/bioinert” – they can be “bioactive”.
• Fortunately, or unfortunately, after most cells adhere to a substrate, they begin to secrete their own matrix (especially fibronectin), which pretty much wipes out any specificity the biomaterial may provide.