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Wang haitao

March 14 2011

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• Loss of one type of sensory input can cause improved functionality of other sensory systems. Whereas this form of plasticity, cross-modal plasticity, is well established, the molecular and cellular mechanisms underlying it are still unclear.

• blind individuals compensate their lack of visual input by responding to somatosensory or auditory input with improved sensitivity and accuracy. The brain can adapt to sensory deprivation in one modality (e.g., visual cortex) by increasing plasticity and retuning neuronal circuits in other remaining modalities.

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• GluR2-containing receptors

• GluR2-lacking receptors

Rectification index= I (-60 mV)/ I(+40mV)

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Methods

• In vitro whole cell recording

• Infection of Neocortical Neurons In Vivo

• In Vivo Microdialysis

• In Vivo Extracellular Recording

• Biochemical Analysis

• Immunohistochemistry

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VD Drives GluR1 into Layer 4 to Layer 2/3 Synapses of the Barrel Cortex in Juvenile Rats

GFP-GluR1

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VD Drives GluR1 into Layer 4 to Layer 2/3 Synapses of the Barrel Cortex in Juvenile Rats

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VD Procedure for 2 Days Occludes LTP in the Barrel Cortex

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VD did not alter properties of NMDA component and inhibitory component

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VD Procedure for 2 Days Occludes LTP in the Barrel Cortex

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Serotonin Mediates VD-Driven Synaptic GluR1 Delivery in the Juvenile Rat Barrel Cortex

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Serotonin Mediates VD-Driven Synaptic GluR1 Delivery in the Juvenile Rat Barrel Cortex

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Serotonin Facilitates Synaptic GluR1 Delivery

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Serotonin Facilitates Synaptic GluR1 Delivery

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Serotonin Facilitates Synaptic GluR1 Deliveryc

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VD Induces Phosphorylation of GluR1 at a Site Critical for Its Synaptic Delivery

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VD Sharpens Functional Whisker-Barrel Map

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• Visual deprivation (VD) drives AMPA receptors into synapses in the rat barrel cortex

• Seoronin mediates VD-induced facilitation of synaptic AMPA receptor delivery

• VD increases activation of ERK and increase of phosphorylation of GluR1 at Ser845

• VD sharpens the map in the barrel cortex

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Thanks