101-9118340612010923-biotech lifesciences catalogue 20102011

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    Biotechnology

    Life Sciences

    Biotechnology / Life Sciences Department

    General laboratory Bioprocessing

    Bioengineering Genetics Chemistry

    Product Catalog 2010-2011

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    Biosafety cabinets (Laminar Flow)

    Centrifuges

    Laboratory freezers

    Freeze Drying

    Content

    GENERAL LABORATORYGENERAL LABORATORYGENERAL LABORATORYGENERAL LABORATORY

    BIOBIOBIOBIO ENGINEERINGENGINEERINGENGINEERINGENGINEERING

    GENETICSGENETICSGENETICSGENETICS

    CHEMISTRYCHEMISTRYCHEMISTRYCHEMISTRY

    CO2 incubators

    Circulators, Open Water baths and Cryo baths

    Bioreactors

    Laboratory ovens

    Shakers

    Thermal cyclers (PCR)

    Polarimetry

    Refractometry

    3

    7

    14

    14

    9

    10

    8

    11-13

    9

    4-5

    6

    Page

    8

    Gel electrophoresis Systems 7

    BIOPROCESSINGBIOPROCESSINGBIOPROCESSINGBIOPROCESSING

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    General Laboratory

    Laboratory cooling

    UPUL SERIESUpright ULT Freezer

    Single compressor Low energy consumption Low noise level 100% HCFC/CFC free Low Heat dissipation Inner doors Castors and door key lock Stainless steel interior &Stainless steel interior &Stainless steel interior &Stainless steel interior & exteriorexteriorexteriorexterior

    Specifications:

    Heated vacuum valve to prevent a vacuumwhen opening the door at short intervals

    Digital controller with visual + acoustic alarms,remote monitoring and 72 hours battery

    backup

    Each freezer shelf is designed with holes foreasy access of CO2 liquid from top to bottom

    Power consumption (kWh/24 h):Power consumption (kWh/24 h):Power consumption (kWh/24 h):Power consumption (kWh/24 h): 11111111

    More environmentMore environmentMore environmentMore environmentArctiko would like to contribute to a more sustainable behaviour. That is why the

    CO2Care concept has been introduced.More profitMore profitMore profitMore profit

    Less emissions of CO2 serve both the environment and the profit.

    Less energyLess energyLess energyLess energyOur CO2 care products are not only combining aesthetics and function, they are

    noted for being extremely energy efficient compared to other products on the

    market. That means less CO2 emission and a s ignificant saving on the electrical

    bill.

    More responsibilityMore responsibilityMore responsibilityMore responsibilityCO2 is the major culprit when it comes to climate changes.

    Ultra lowtemperature

    UprightULUF 400-450-800-850

    UPUL (SS) 540-580

    ChestULTF 80-320-420

    OtherCryo Portor

    MUF 33

    Low temperature

    UprightUPLTF 90-275

    LF 140-300-500-660-

    1400

    PF 500-700-1400

    ChestLTF 85-325-535

    LTFE 140-290-370

    Refrigerator

    LaboratoryLR 170-270-300-390-500-

    660-700-1400

    PharmaPR 170-360-390-500-700-

    1400

    Blood & PlasmaBBR 500-700-1400

    CO25DC

    TE21AC

    PBF 108-116-124

    APPLITEK BIOTECH & LIFE SCIENCES GENERAL LABORATORY 3

    NEW

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    Laminar Flow cabinets

    Fortuna Class IClass IClass IClass I Cabinets:

    Advanced design for a clean working

    environment. The Fortuna is a clean air cabinet

    with vertical laminar flow, designed for the

    ultimate product protection due to its unique

    laminarity

    Optimal Operator Comfort Low running costs due to strong fans,

    low volume cabinet construction and

    low energy transmission

    Operator-friendly microprocessorcontrol

    Designed for most applications

    Mars Class IIClass IIClass IIClass II cabinets provide Optimal Operator Comfort Maximum Operator Safety Energy Saving Benefits with the especially designed EC-

    fans which also provide lower noise level

    Operator-friendly microprocessor control Eco-Save AutoStart AutoStop option Variety of options, including gas valves, Bunsen burner,

    heated work top panel, marble insert for weighing

    applications, built-in computer screen

    Versions:

    Type ApplicationMars-IVF In vitro

    Fertilization

    Integrated microscope and

    heated/cool platens

    Mars-Weigh

    Master

    Precise

    weighing

    Marble insert platform and

    integrated computer screen

    Mars-Iso Isotope Lead protection and Lead

    glass to all windows

    Orion Class II 100% exhaustClass II 100% exhaustClass II 100% exhaustClass II 100% exhaust cabinet:

    Chemistry Inflammable liquids Chemicals and acids High concentration of organic

    solvents explosion proof

    version (a class II and fume

    hood in one)

    Animal Handling Surgery Cage Change Research (Manipulation)

    General High concentration of odours Where recirculation can cause

    disruption or disturbances

    4 GENERAL LABORATORY APPLITEK BIOTECH & LIFE SCIENCES

    Class I

    FortunaUltimate product

    protection

    Titan

    Designed for PCR andapplications requiring

    sterility better than

    Class 100

    AtlasAdvanced design

    providing operator

    and environment

    protection with built-

    in exhaust fan

    XITPortable cabinet with

    recirculatory filtration

    EXCELProviding horizontal

    airflow away from the

    operator

    Class II

    MarsOperator, product and

    environment

    protection with

    recirculatory filtration

    Mars Pro3 HEPA-filters

    providing 99.999%

    efficiency

    SIRIUSDesigned for Class 2

    work and handling of

    medicine and

    cytotoxics

    ORION100% exhaust for work

    with animal handling

    or high

    concentrations of

    organic solvents

    SPECIAL

    DISCOUNTS

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    Powder Handling & Containment Systems

    XIT XIT Plus Excel Plus

    Features

    Compact size Quiet operation, less than

    50d(B)A and low power

    consumption

    Fan speed pre-set foroptimum face velocities of

    0.3 to 0.7 ms-1

    Features

    Same features as XIT and Safechange pre-filter bagged

    under negative pressure

    Chemcap filter for chemicalfume removal (Chemcap filter

    type depending on Risk

    Assessment Form)

    Features

    Aerofoils ensure an even flow across thewidth of the cabinet and the best

    containment possible

    A horizontal flow of airhorizontal flow of airhorizontal flow of airhorizontal flow of air, this flow can beset to a very low velocity, ideal for your

    precision weighing jobs

    Quiet operation, less than 50d(B)A andlow powerconsumption

    Enclosures

    Features:

    Class II airflow patttern technology forbiohazards

    Bigneat downflow air-draw protectiontechnology

    Gives protection from moving parts,radioactivity and UV

    Mobile or easily relocatable Ductless installation does not affect pressures

    and flows in existing ducted systems

    Avoids need for new cleanroom facilities

    Releases fume cupboard space All-round visibility Compact enclosure solution

    Options:

    Climatic control Slide out trays and shelves

    (easy removal of equipment, access when

    servicing)

    Emergency stop control Mechanical and electronic interlocks Variety or door arrangements

    (sliding, wardrobe, bi-folding, vertically folding)

    Leaded acrylic windows (for protection fromradiation)

    Controlled atmosphere UV lamps

    APPLITEK BIOTECH & LIFE SCIENCES GENERAL LABORATORY 5

    XIT

    supplied with

    pre-filter and HEPA filter

    dimensions

    (W x D x H):

    415 x 575 x 455 or

    579 x 506 x 455

    XIT Plus

    supplied with

    pre-filter and HEPA filter.

    Low Flow Alarm

    included

    Dimensions from 770

    mm to 1170 mm inwidth

    UV Sterilisation cabinet

    and PCR Workstation

    Supplied with pre-filter

    and HEPA-filter on PCR

    cabinet only

    *Powerful UV-lights

    *All-round vision without

    transmission of UV light

    Excel Plus

    supplied with

    pre-filter and HEPA filter.

    Ducted Model.Ducted Model.Ducted Model.Ducted Model. .Low

    Flow Alarm included.

    Dimensions from 770

    mm up to 1470 mm in

    width

    Laminar Flow Cabinet

    with Horizontal Clean Air

    Operator or product

    protection

    Dust and Fumecontainment systems

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    Centrifuges

    Micro-centrifuge

    Mini13.500 RPM

    Acceleration time:

    12 secDeceleration time:

    16 sec

    1524M15.000 RPM

    5 Acceleration &

    Deceleration levels

    1730MR17.000 RPM

    5 Acceleration &

    Deceleration levels

    Low SpeedCentrifuge

    406G4.000 RPM

    Acceleration time:

    20 sec

    Deceleration time:

    20 sec

    416G4.000 RPM

    Acceleration time:

    20 sec

    Deceleration time:

    20 sec

    Multi-PurposeCentrifuge

    1236MG12.000 RPM

    5 Acceleration &

    Deceleration levels

    1580MGR15.000 RPM

    5 Acceleration &

    Deceleration levels

    Main features of ScanSpeed centrifuges:

    Quiet operation by way of a high torque AC induction motor The unique design of air-flow in non-refrigerated centrifuges protects samples from over-heating even at

    high temperatures

    Noise level lower than 60dB Easy to follow display and control panel Automatic RPM/RCF conversion Automatic detection with alarms for over-weight, over-speed and over-heating Safety lid-lock and lid-drop protection Emergency door-lock release (in case of power failure) Autoclavable and corrosion-resistant rotors with symmetrically numbered tube positions for easy loading Manufactured and tested according to the IEC 61010-20020 standards and regulations

    Send us your tubes and we will custom-make

    your ScanSpeed centrifuge adapters

    6 GENERAL LABORATORY APPLITEK BIOTECH & LIFE SCIENCES

    NEW

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    Genetics

    Thermal Cycler

    GeneQ

    Volumes:

    18 x 0.5 ml

    24 x 0. 2ml (8 strip - 24-

    microplate)

    Life Pro

    96-microplate

    96 x 0.2 ml

    12 x 8-strip

    Gene Pro

    96-microplate

    96 x 0.2 ml

    12 x 8-strip

    30 x 0.5 ml

    384-microplate

    ElectrophoresisSystems

    Mini-Run

    The GE-100 apparatuscan be used for agarose

    gel electrophoresis.

    Different gel sizes and

    combs are available.

    BioTANON

    Horizontal system

    Different gel sizes and

    combs are available to

    choose from. This allows

    for a versatile system

    with choice of sample

    volume.

    Mini vertical system

    The gel size of 8.3 x

    7.3cm (W x L) can be

    used with different

    combs to change

    sample volume per well

    Mini blotting system

    The blotting system allows

    for 2 gels(7.2 x 10) to be run

    at the same time.

    Thermal Cyclers Ultra low noise design Adjustable hot-lid pressure Gradient function Large screen USB storage function Network function Fast ramping rate (up to 4C/s for cooling or heating)

    GeneQ Life Pro Gene Pro

    Temp. Range 4~99,9C 4~99,9C 4~99,9C

    Heating rate 5C/sec 4C/sec 4C/sec

    Cooling rate 4C/sec 4C/sec 4C/sec

    Block temp. Uniformity 0,2C 0,2C 0,2C

    Gradient range N/A 1~30C 1~30C

    Electrophoresis Systems

    Horizontal systemagarose gel electrophoresis

    Vertical system(SDS) PAGE electrophoresis

    APPLITEK BIOTECH & LIFE SCIENCES GENETICS 7

    Ultraviolet products and Accessories

    Ultraviolet crosslinker Hybridization Ovens

    Applications

    Shortwave 254nm UV: UV crosslinkingmembranes

    Longwave 365nm UV: UV curing, non-destructive testing

    Midrange 302nm UV: Photochemicalreactions, UV crosslinking

    The Vertical System is idealfor casting and running

    SDS-PAGE gels. The Blotting

    system provides rapid, high-

    quality blotting of mini gels.

    The precision heated and multifunctional hybridization

    ovens feature accurate temperature and motion

    controls, easy clean interior, stainless steel construction,

    and a wide selection of models to meet individual user

    or laboratory budgets and requirements. The dynamic

    mixing capability is ideal for creating optimum

    conditions needed for cDNA library screenings, primersynthesis and nucleicacid hybridization.Next to these products, we can also provide you with UV transilluminators,

    UV lamps, UV meters, UV incubators and other Ultraviolet products.

    NEW

    The Horizontal System is idealfor casting and running

    agarose gels. Supplied with a

    Gel Caster which eliminates

    the need for gel sealing tape

    during gel casting.

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    Bio Engineering

    Freeze Drying

    CoolSafeLaboratory Freeze dryers

    CoolSafe

    Temperature

    -55 C*-95C*

    -100C*

    -110C*

    *at 20C room

    temperature

    Volume (L)

    4, 9 & 15

    MaxiVac

    Alpha BetaHeatSafe

    Volumes (L)

    4, 6, 8, 18 & 28

    ChillSafe

    Volumes (L)

    8 & 18

    Option

    Power: extra strong

    external circulation

    pump for cooling

    down over long

    distances such as with

    mass spectro-

    photometres

    Cryosafe

    Temperature range

    -50C to +150C

    -90C to +150C

    Volumes (L)

    8 & 18

    Specifications

    seamless condenser with external cooling coil Wide range of Freeze Dryers Teflon coating (can work with very aggressive solvents and acids, e.g. TFA, DMSO, etc.) De-icing is very simple due to the external cooling coil Bench top or Floor standing Different Control options (Basic, PRO, PC option) Wide range of Accessories: Manifold with Cones for freeze drying flasks; Small Chamber

    Accessories; Unheated Shelve Systems; Electrically Heated Shelves; etc.

    MaxiVacCentrifugal evaporation

    Alpha: Your solution for fast macro sample vacuum concentration

    Beta: Your multi-purpose solution for low temperature macro sample

    vacuum concentration and freeze drying

    VacSafe

    Vacuum Pumps

    Circulators, Open Water Baths and Cryo BathsCirculators, Open Water Baths and Cryo BathsCirculators, Open Water Baths and Cryo BathsCirculators, Open Water Baths and Cryo Baths

    Circulators

    Versatile stand alone circulators for existing or new heated, cooling and cryo baths mounted on

    bridges or clamps

    HeatSafe

    Powerful heating water baths available in different volumes with strong pump in highest Safety

    Class 3 and high heating capacity of 2000 watt for fast heat up. Temperature range from +25C to

    +150C

    ChillSafe

    Powerfull cooling chiller and bath with cooling capacity of 300 watt at 20C with strong pump in

    highest Safety Class 3 and high heating capacity of 2000 watt. Temperature range from -35C to+150C

    CryoSafe

    Intelligent low temperature controlled cryo baths with excellent temperature control

    8 BIO ENGINEERING APPLITEK BIOTECH & LIFE

    NEW

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    By providing more inc ubation c apacitymore incubation capacitymore incubation capacitymore incubation capacity,

    in a smaller footprint, with more options

    and a significant decrease i n risk ofdecrease in risk ofdecrease in risk ofdecrease in risk of

    contaminationcontaminationcontaminationcontamination compared to water-

    jacketed systems, Galaxy CO2 incubators

    are easier to maintain, setup and utilize,

    while providing superi or p erformancesuperior performancesuperior performancesuperior performance.

    These elements have made Galaxy

    systems a favourite among discerning

    scientists in in-vitro fertilization (IVF),

    stem cell research, as well as traditional

    cell culture.

    Laboratory Ovens

    ThermoCenter

    The ThermoCenter is an extremely versatile oven. It can be

    used in R&D, QC as well as industrial applications. A must

    when uniform temperature d istributionuniform temperature distributionuniform temperature distributionuniform temperature dist ribution and a highhighhighhigh

    accuracyaccuracyaccuracyaccuracy are needed.

    IncuCenter

    The IncuCenter is a very versatile incubator. It can be used

    in R&D, QC and industrial applications. This incubator is

    advised when precise temperature distributionprecise temperature distributionprecise temperature distributionprecise temperature distributi on and a

    very high accuracyvery high accuracyvery high accuracyvery high accuracy are needed.

    Vacucenter

    The VacuCenter Line is designed for all purposes of

    vacuum dryingvacuum dryingvacuum dryingvacuum drying in a variety of laboratory fields. All systems

    have a controller with alphanumeric display and

    programming capabilities. Temperature ranges up to

    200C.

    Biocenter

    The BioCenter is a CO2 incubat orCO2 incubatorCO2 incubatorCO2 incubat or designed to ensure

    accurate and very reliable control of CO2, temperature and

    humidity.

    CO2 incubators

    Bioprocessing

    CO2 incubators

    Volumes 14-170L,

    up to 50C

    Control of

    CO2 (Uniformity0.1%)

    Humidity (up to 95%)Temperature

    (Uniformity 0.4C)

    Options:

    O2 control 72-hour data loggingLaboratory Ovens

    ThermoCenter:

    Determinetemperature,

    temperature gradient,

    timing and air

    throughput Volumes 40-400L,

    up to 285C

    IncuCenter

    Precise temperaturecontrol and process

    monitoring

    Volumes 40-400L,up to 110C

    Vacucenter

    Control temperature,timing and

    evacuation level

    Volumes 20-50L,up to 200C

    Biocenter

    Constant conditionsfor CO2, temperature

    and humidity

    Volume 175L, up to50C

    APPLITEK BIOTECH & LIFE SCIENCES BIOPROCESSING 9

    Laboratory heating

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    LS-X / EX-X

    These table top Lab-

    shakers are very reliable

    bench shaking units

    made for continuous

    maintenance free

    operation with the

    extremely efficient

    Kuhner magnetic direct

    drive.Tray sizes:

    E 420 x 420 mm

    EX 500 x 420 mm

    F 820 x 420 mm

    LT-X

    The Lab Therm X (LT-X)

    incubator shaker.

    Certainly the most

    reliable table top Shaker

    incubator available

    today. With the same

    features and options as

    the ISF1-X. Also

    stackable (2 units) with a

    king size EX tray of

    500x420 mm

    ISF1-X

    The ISF1-X stackable

    incubator shaker. Up to3 shakers use the same

    floor space as 1 unit. For

    ease of sterilizing and

    cleaning an all stainless

    steel interior. Very large

    front windowdoor for

    loading and observation

    of the experiments at

    close proximity without

    having to open the door

    ISF4-X

    The ISF4-X incubator

    shaker cabinet. The ideal

    replacement or addition

    to your environmental

    rooms. Large shaking

    capacity on a small floor

    space. Up to 4 or 5

    completely

    independent shaking

    units with individual

    height settings. As with

    all Khner shaker

    incubators, an all

    stainless steel interior

    10 BIOPROCESSING APPLITEK BIOTECH & LIFE SCIENCES

    Shakers

    Benchtop shakers

    Shaker-incubators

    Accessories

    LS-X ES-X

    SHAKER TECHNOLOGY:

    Built for continuous operation with a magnetic D.C. direct drive. Designed especially forshakers, extremely reliable, smooth running and quie tquietquietquiet whisper. Ver yVeryVeryVery energy efficientenergy efficientenergy efficientenergy efficient.

    Change of shakingChange of shakingChange of shakingChange of shaking diameterdiameterdiameterdiameter possible.possible.possible.possible. The single eccentric system can be changed to3 different positions (Standard 12,5 25 50 mm) but other diameters possible)

    NNNNo maintenanceo maintenanceo maintenanceo maintenance requiredrequiredrequiredrequ ire d. Designed to operate 24 hours a day for years to come. Orbital OR linear movement

    CoolingCoolingCoolingCooling for temperatures below ambient. Controlled humidityControlled humidityControlled humidityControlled humidity with sensor, automatically refilled

    water bath and window/door heating. Controlled CO2Controlled CO2Controlled CO2Controlled CO2 up to 20% CO2 for mammalian cells.

    PhotosynthesisPhotosynthesisPhotosynthesisPhotosynthesis. UVUVUVUV lamp for sterilization. Darkened windowDarkened windowDarkened windowDarkened window. Pull out platformPull out platformPull out platformPull out platform. Dual tableDual tableDual tableDual ta ble: 2 levels

    with a distance of 60/85/120mm allows the skaking of two trays.

    LT-X ISF1-X ISF4-X

    Trays withTrays withTrays withTrays with fixed stainless steel clampsfixed stainless steel clampsfixed stainless steel clampsfixed stainless steel clamps for Erlenmeyer-flasks, Fernbach-flasks, test-tube holders,

    microtiter or deep well plates or any other kind of container. Trays with a washable self sticking matTrays with a washable self sticking matTrays with a washable self sticking matTrays with a washable self sticking mat

    for shaking of Erlenmeyer flasks with speeds under 200 rpm. Trays with horizontal rubber coveredTrays with horizontal rubber coveredTrays with horizontal rubber coveredTrays with horizontal rubber covered

    bars or traysbars or traysbars or traysbars or tra ys for separating vessels. Univers al tr aysUniversal traysUniversal traysUnivers al tr ays with treads which can be used with clamps from

    25ml to 6000ml or adjustable test-tube holders.

    The ES-X is especially designed for

    installation in incubators. Intended

    for working in high temperaturesand high humidity.

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    - Flask

    24 (or 96) singleuse and low costbioreactors permicrotiter plate

    No well-to-wellcrosscontamination

    Low evaporation Anaerobic (or high

    CO2) cultivations

    FlaskRAMConsistent growthConsistent growthConsistent growthConsistent growthand high cell densiand high cell densiand high cell densiand high cell densitiestiestiestiesbecause ofbecause ofbecause ofbecause of superiorsuperiorsuperiorsuperioroxygen transfer.oxygen transfer.oxygen transfer.oxygen transfer.Higher cell densityHigher cell densityHigher cell densityHigher cell densityand improvedand improvedand improvedand improvedconsistency = betterconsistency = betterconsistency = betterconsistency = betterprediction ofprediction ofprediction ofprediction ofdownstream stepsdownstream stepsdownstream stepsdownstream steps

    High volume flasks Less sensitive to

    higher viscositymedia bettermixing of thick mediatypes

    6 fold better OTRsachieved comparedto orbital shakers dueprimarily to twothings:1.The power of theacoustic mixing

    2.Active headspacerefreshment by theOxyPump

    AFS(AlcoholFermentationSystem)

    Robust and user-friendly parallelbioreactor for sixsimultaneous

    anaerobic yeastfermentations

    Highly accuratecarbon conversiondata, allowingresearchers toeasily monitorand improve theirprocess

    Accuratecomparisons ofconversion ratesand yields underdifferent

    conditionssuch astemperature,strain type, carbonsource ornutrients

    ---- Flask systemFlask systemFlask systemFlask system FlaskRAMFlaskRAMFlaskRAMFlaskRAM AFSAFSAFSAFS

    Features:

    Function Cultivation system with 24(96) individual micro-

    reactors

    Mixing incubator forgrowing flask cultures

    Monitoring anaerobic,metabolic yeast activity

    for alcohol production

    Capacity

    24 (or 96) wells per

    microtiter plate

    (up to 4 plates in 1 clamp)

    18 x 250 ml

    14 x 500 ml

    8 x 1000 ml

    6 x 250 ml

    6 x 500 ml

    Shaking Orbital Shaker Resonant Acoustic Mixing Magnetic Stirrer

    Oxygen

    Transfer Rate Up to 51 mmol O2/l/h

    Up to 240 mmol O2/l/h

    (with OxyPump)

    Dependable on stirrer

    speed

    Monitoring N/A N/A Carbon conversion

    Control N/ATemperature and

    HumidityMixing and Temperature

    Application areas:

    Target culture Microbial & Cell Microbial Yeast

    Examples

    *DiscoveryDiscoveryDiscoveryDiscove ry of secondarymetabolites or new

    enzyme activities

    *High throughput

    screeningscreeningscreeningscreen ing and construct

    libraries

    *Metabolic fluxMetabolic fluxMetabolic fluxMetabolic flux studies

    *ComparativeComparativeComparativeComparative studies

    *Growth mediumGrowth mediumGrowth mediumGrowth medium

    optimizationoptimizationoptimizationoptimization

    *Shake flask experiments

    employing vis cous orviscous orviscous orviscous or

    hard to mixhard to mixhard to mixhard to mix mediamediamediamedia

    e.g. Biofuels applications

    (aerobic though!),

    Streptomyces

    *Large volume flaskLarge volume flaskLarge volume flaskLarge volume flaskgrowthgrowthgrowthgrowth

    e.g. pilot plant seed

    cultures, research aterialproduction cultures

    *Measure conver sionconversionconversionconversion

    ratesratesratesrates of

    -lignocellulose

    hydrolysates into biofuels

    - wort into beer

    - grape juice into wine

    *Test and comparecomparecomparecompare

    - yeast strains

    - feedstock/substrates

    *QQQQualityualityualityuality control

    *Alcohol tolerance andAlcohol tolerance andAlcohol tolerance andAlcohol tolerance and

    toxicitytoxicitytoxicitytoxicity studies

    Small Scale

    APPLITEK BIOTECH & LIFE SCIENCES BIOPROCESSING 11

    Bioreactor systems

    NEW

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    Laboratory Scale

    Glass Autoclavable Systems

    Single-Use AppliFlex

    Autoclavablesystems

    Available in singlewall and in jacketedversions. Up to 300Up to 300Up to 300Up to 300different modulesdifferent modulesdifferent modulesdifferent modulesare available tocustomizecustomizecustomizecustomize yourbioreactor system.

    ModulesGas inlet and outletsystems; samplesystems; liquidaddition systems;agitators withvariety of impellers;liquid storagesystems; heatexchangers; baffles

    Single use

    The driving forcebasically iseconomical:

    * Single-useproducts overcomethe costly cleaningprocedures that areinvolved in re-usabletechnologies.Various studies

    describe andcompare these costeffects

    * The lack ofvalidation issuesallows a shortertime to market forproducts producedin single-usesytems.

    SIP

    All internal parts inthe bioreactorshave anelectropolishedelectropolishedelectropolishedelectropolishedfinishfinishfinishfinish with an Ravalue of less than0.4 micrometer toensure reliablecleanability of thevessel andcomponents. Allstainless steelbioreactors andfermentors can beused incombination with aCleanCleanCleanClean----InInInIn----PlacePlacePlacePlace

    system.

    The systems are built according to the specific demands o f a process with

    our standard elements. Because of the modularity and flexibility, the user

    can always adapt the systems to changed process demands. This results in

    low initial investment and low running costs.

    Culture- Microbial

    - Cell

    Volume (L) 1 2 3 5 7 15 20

    Aeration

    - Stirrer

    - Rotameter (up to 4)

    - Mass Flow Controller (up to 4)

    Temperature

    regulation

    - Heating blanket (optional

    cooling valve)

    - Thermocirculator

    Application

    Process optimization, screening studies,

    product optimization, scale up/scale down

    studies

    Lipseal or

    Magnetic stirrer

    Benefits of single-use bioreactors include faster facility set-up, reduction

    of down time, simplified validation, and more efficient use of plant floor

    space. Disposable bioreactors greatly reduce the risk of cross-

    contamination in multi product facilities.

    Culture Cell

    Volume (L) 10 20 50

    Aeration- Rocking

    - Gas in- and outlet lines

    Application Mammalian cell based biotech

    Stainless Steel Steam-In-Place Bioreactors

    The BioBench bioreactors and fermentors are designed for research

    applications such as process optimization.

    Culture- Microbial

    - Cell

    Volume (L) 7 15 20 30

    Aeration

    - Stirrer

    - Rotameter (up to 4)

    - Mass Flow Controller (up to 4)

    ApplicationProcess optimization, screening studies, product

    optimization, scale up/scale down studies

    12 BIOPROCESSING APPLITEK BIOTECH & LIFE SCIENCES

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    Production ScalePilot Systems

    Projects

    In addition to our range of standard stainless steel bioreactors, we offer custom designed

    systems. Please contact us for more information.

    Process Control

    BioSep

    The upscaling of the SIP bioreactors provide the same features as

    the Bio Bench bioreactors.

    Culture

    - Microbial- Cell

    Volume (L)Microbial: 20 40 70 140

    Cell: 30 60 130

    Stirring

    - Magnetically coupled stirrers- Top stirrers- Bottom drives

    The ez-control regulates pH, Temperature, Dissolved Oxygen,

    Foam/Level and Agitation. The colour touch screen interface

    easily guides the user through operation of the controller. The

    adaptive control features allow the user to focus on the process

    while the controller keeps tight control on the important

    process parameters. The system allows easy software updates

    and communication through the integrated web server. The

    optional ADDA board offers an additional 8 analog inputs, 4

    analog outputs, 8 digital inputs and 8 digital outputs.

    BiosepBiosepBiosepBiosep is an acousticacousticacousticacoustic separationseparationseparationseparation devicedevicedevicedevice. The

    separation principle is purely based on gentle

    acoustically induced loose aggregation

    followed by sedimentation.

    PerfusionPerfusionPerfusionPerfusion isisisis thethethethe technologytechnologytechnologytechnology totototo use,use,use,use,

    providingprovidingprovidingproviding itsitsit sits users:users:users:users:

    High cell density (over 10 mio cells/ml) High (volumetric) productivity: required

    bioreactor volume can be reduced

    dramatically (100-fold)

    Cost-effective operation (reduced laborrequirements for bioreactor inoculation

    and turnaround)

    Pilot system

    An electropolished

    finish with an Ra

    value of less than 0.4

    micrometer and an

    external, mirror-

    polished finish of all

    the standard

    systems. All stainless

    steel bioreactors andfermentors can be

    used in combination

    with a CleanCleanCleanClean----InInInIn----

    PlacePlacePlacePlace system.

    The process

    parameters of the

    systems can be

    controlled by both

    ez-Control and i-

    Control systems.

    Ez-Control

    Easy operation

    Security

    Easy updates

    Advanced computer

    interface

    Easy communication

    Expandability

    The i-control is

    available for industrial

    bioreactor control

    system and utilityconsole

    BioSep

    Using high frequency

    resonant ultrasonic

    waves to separate cells

    from product instead of

    physical mes hor

    membrane, it offers all

    the benefits of

    traditional devices butwithout their inherent

    problems and

    limitations.

    Applications of BioSep:

    - Cell retention

    - Cell concentration

    - Cell washing

    R & D (min. 1L/day),

    process development

    (50 L/day) and

    production scale

    (1000L/day).

    APPLITEK BIOTECH & LIFE SCIENCES BIOPROCESSING 13

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    Polarimetry

    *Full range of singl esinglesinglesingle

    wavelengthwavelengthwavelengthwavelengthpolarimeters

    *NIR SaccharimetersSaccharimetersSaccharimetersSaccharimeters

    *Dual wavelengthDual wavelengthDual wavelengthDual wavelength

    high accuracy

    polarimeters

    *Multi wavelengthMulti wavelengthMulti wavelengthMulti wavelength

    high accuracy

    polarimeters

    *Comprehensive

    range of glass, PTFE

    and stainless steel

    sample tubes

    *Full range of

    sample tubessample tubessample tubessample tubes in

    both jacketed and

    unjacketed form

    *thermotubes

    *Complete range of

    quartz control plates

    *Instruments and

    quartz plates

    available with UKAS

    certificates

    Refractometry

    *GPR series are

    temperature

    controllable, to both

    prism and sample

    cover, by flowing

    fluid from a

    thermocirculater

    temperature of

    5C~70C

    * PTR series

    incorporating

    electronic

    temperature control

    by Peltier Cell

    technology

    * Three models of

    ATM X2 colorimeters

    available, designed

    specially for sugar

    colour measurement.

    14 CHEMISTRY APPLITEK BIOTECH & LIFE SCIENCES

    Chemistry

    Polarimetry

    The PolAAr series of polarimeters and

    saccharimeters consists of eight models

    with the options of single wavelength,

    dual wavelength or multi wavelength, a

    choice oftwo or three figure accuracy,

    and simple or full alphanumeric keypads.

    Thermotubes

    Accurate temperature control without athermocirculator

    100 mm and 200 mm path lengths 8 mm and 4 mm diameter bore sizes Can be used in all our polarimeters Temperature range 15C to 30C

    Refractometry

    General Purpose refractometers (GPR)

    Peltier controlled refractometers (PTR)

    Visual/digital handheld refractometers

    High temperature refractometers

    Special purpose refractometers

    ICUMSA colorimeters

    UKAS calibration standards

    GPR series refractometer with RIRIRIRI, BrixBrixBrixBrix (sucrose percent), temperature corrected Brix, temperaturecorrected RI and up to 10 calibrated scales are available.

    PTR series refractometers with accurate temperature control, wide range of RI (1.32 - 1.68) and Brix (0 100%), resolution up to 0 .00001 RIresolution up to 0.00001 RIresolution up to 0.00001 RIresolut ion u p t o 0 .00001 RI, temperature range from 15C to 50C, multi-scale models and

    full GLP capability

    The GPR 12-70E refractometer is especially suited to high temperature measurements up to 100Cmeasurements up to 100Cmeasurements up to 100Cmeasurements up to 100C. Special purpose refractometers for the soft, carbonated and alcoholic beverage industries, for the

    anaesthetic and petrochemical gas industries and for the contact lens industry.

    Single and Dual wavelength ColorimetersColorimetersColorimetersColor imeters conform to I.C.U.M.S.A. method GS2/3-18 withwavelengths 420, 560 and 720 nmwavelengths 420, 560 and 720 nmwavelengths 420, 560 and 720 nmwavelengt hs 4 20, 560 and 720 nm. Highest accuracy for white sugar and longest path length 163

    mm.

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    APPLITEK BIOTECH & LIFE SCIENCES 15

    AppliTek services & support at a glance

    Belgium

    Our people at your disposal Before and after purchase

    The Biotechnology / Life Sciences department has its own entity inside

    AppliTek. The sales and technical people you are in touch with only

    deal with the products you rely on. A single point of contact is at your

    disposal for product information, technical or commercial issues. For

    urgent matters, you will be contacted to schedule assistance or

    intervention, if needed.

    In our documentation (written and electronic) we guarantee up to

    date information about the product that you are looking for. All

    information carrying specific details includes updated pictures. When

    more information on a topic or system is required, our support team is

    only a phone call away.

    Value added services for support and maintenance

    When you rely on AppliTeks portfolio for your research and lab

    projects, you rely on premium quality products from established

    manufacturers. On top of that, you can be assured that your facility is

    in good hands. By working closely in collaboration with our suppliers,

    we can provide you tailored service agreements and preventive

    maintenance for your equipment and systems.

    Language support: English Nederlands franais

    Visit our renewed websitebiotech.applitek.com

    Browse through our portfolio of lab equipment,specialty systems and accessories

    Exclusive and limited offersRequest for quotation with priority through

    AppliTek contact form

    Receive our bimonthly newsletterContact details of our employees

    Maintenance Intervention Checks

    Personal contact Swift information

    O n - l i n e

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    AppliTek

    Biotechnology / Life Sciences

    Certified Sales & Service for Belgium

    2010 AppliTek NV Printed in Belgium

    AppliTek NV/SA

    Industrial Zone De Prijkels

    Venecoweg 19

    B-9810 Nazareth

    Belgium

    Tel.: +32 9 386 34 02

    Fax.: +32 9 386 72 97

    E-mail: [email protected]

    Visit us on biotech.applitek.com

    AppliTek in Belgium Products, systems and accessoriesfor R&D, routine and process applications for the

    biotechnological and pharmaceutical sectors

    The Biotechnology/Life Sciences division of AppliTek concentrates since 1985

    on the advice, sales and installation of products and systems designed for the

    pharmaceutical industry, biotechnology companies and research faci lities.

    AppliTek has a name one can trust in the pursuit of a long-term mutual

    cooperation. For its top-notch suppliers, AppliTek is the authorized sales and

    service center with an established infrastructure in the Belgian market.

    Customers can rely on AppliTeks advice and supply of the finest dedicated

    equipment, combined with differentiated and personal after-sales service.