(1)start lasers, then find sample using vis. (2)set configuration using lsm
Post on 21-Dec-2015
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(1)Start lasers, then find sample using VIS
(2)Set configuration using LSM
(3)Set scan resolution, speed & repetition in Mode
Initial pixal number, scan speed, averaging, zoom and pinhole size are set in stored configurations. These should be optimised after preliminary images.
(4)Set pinhole, excitation & detection for each Channel
Single track
Multi trackUse Find to get initial image, Split and Palette to adust
(5)Control Zoom and Crop in Mode and Select
(6)Set Z stack with first/last & optical slice
(7)Display 3D using projection
(8)Open database for image retrieval
(9)Time Series and Edit Bleaching
(10)Using Lambda stacks, Save to Spectra DB for Linier Unmixing