(1)start lasers, then find sample using vis. (2)set configuration using lsm

10
(1)Start lasers, then find sample using VIS

Post on 21-Dec-2015

220 views

Category:

Documents


1 download

TRANSCRIPT

Page 1: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(1)Start lasers, then find sample using VIS

Page 2: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(2)Set configuration using LSM

Page 3: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(3)Set scan resolution, speed & repetition in Mode

Initial pixal number, scan speed, averaging, zoom and pinhole size are set in stored configurations. These should be optimised after preliminary images.

Page 4: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(4)Set pinhole, excitation & detection for each Channel

Single track

Multi trackUse Find to get initial image, Split and Palette to adust

Page 5: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(5)Control Zoom and Crop in Mode and Select

Page 6: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(6)Set Z stack with first/last & optical slice

Page 7: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(7)Display 3D using projection

Page 8: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(8)Open database for image retrieval

Page 9: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(9)Time Series and Edit Bleaching

Page 10: (1)Start lasers, then find sample using VIS. (2)Set configuration using LSM

(10)Using Lambda stacks, Save to Spectra DB for Linier Unmixing