2007 IUFRO All Division 5 Conference, Taipei5.07.00 B Energy from biomass / Chemicals from wood

The antioxidant activity and protective effect of extracts from the stem of Vitis kelungeusis on carbon

tetrachloride-induced acute liver injury in mice

Department of forestry, National Chung-Hsing University

Kai-Chung Chang

Kai-Chung CHANG, Lie-Fen SHYUR, Chi-Chang HUANG, Shih-Chang CHIEN and Jyh-Horng WU

Experimental and methods

The objective of this study is to investigate and identify the bioactive secondary metabolisms from wild grape. In vitro assay

DPPH radical scavenging assay

Total phenolic contents

In vivo assay

Extracts (10 mg/Kg of BW, i. pi. p..)

Silymarin (50 mg/Kg of BW, i. pi. p..)

Vehicle (Negative control, i. pi. p..)

20% CCl4 in olive oil (40 µL/kg of BW, i. pi. p.)

-2 d -1 d 0 6

hr

Scarified

Male ICR mice

6 weeks old

Kai-Chung Chang

0

20

40

60

80

100

0 10 20 30 40 50

Crude extract EA fraction BuOH fraction

Water fraction Asorbic acid

Concentration (µg/mL)

Inhi

bitio

n (%

)

0

100

200

300

Crude extract

EtOAc BuOH Water

Tot

al p

heno

lic c

onte

nts

(mg

GA

E/g

)

Fractions

DPPH radical scavenging activity of V. kelungeusis extracts

Total phenolic contents of V. kelungeusis extracts

Results

Kai-Chung Chang

In vitro assay The crude extract and its derived soluble fractions showed excellent antioxidant activities.Of these the BuOH fraction was the strongest one.

In vivo assayMice pretreated with crude extract or BuOH fraction (10 mg/kg/day, i.p.) for 3 days, remarkably prevented the elevation of serum AST and ALT activities on CCl4-induced acute liver injury.

Results

Resveratrol derivatives

O

O

OH

OH

OH

OH

OH

OH

O

OH

OH

OH

OH

OH

OH

O

OH

OH

OH

OH

OH

O

OH

OH

OH

OH

(+)-Balanocarpol

0.90%

(-)-ε-Viniferin

6.34%

New compound

0.39%

New compound

2.68%

Kai-Chung Chang

Thanks for Thanks for your your

attention attention

Kai-Chung Chang