2009 nmvb conference nyu dept of cardiothoracic surgery nyu dept of pathology friday december 4 th,...
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2009 NMVB Conference2009 NMVB Conference
NYU Dept of Cardiothoracic SurgeryNYU Dept of Pathology
Friday December 4th, 2009
Personnel @ NYUPersonnel @ NYU• Dept of Cardiothoracic Surgery:
• Harvey Pass, MD (surgeon, NYU steering committee member & PI)
• Audrey Sorensen, RN (research nurse coordinator)
• Samantha Lincoln (data manager)
• Ryan Harrington (lab technician, thoracic lab)
• Angela Pittman (research project coordinator)
• Dept of Pathology:• Jonathan Melamed, MD (pathologist, NYU steering committee member)
• Jacinta Small (lab technician, pathology lab)
• Tian He (research coordinator; previously Timothy Jolis)
• Xiangtian Kong (pathologist, pathology review)
2008-20092008-2009• Logistic improvements:
• Streamlined specimen/case-notification process
• Refined tissue procurement process with
response team and procurement kits
• Synchronized local data
with MVB database
• Progression on 5-year plans:
• Frozen TMA
• NYU MVB Paraffin TMA 1
Prospective cases: 63Retrospective cases: 157 Total # of cases: 220
YearRetrospective
CasesProspective
CasesOverall Total
2007
157 31 188
2008
0 21 21
2009
0 11 11
Total: 220
Mesothelioma AccrualMesothelioma Accrual
Tissue Collected Tissue Collected (research blocks)(research blocks)
Collection Method # of Blocks / Samples of Frozen Tissue
OCT 124
FOIL 130
Paraffin Blocks 288
Year New cases collected
Collection Method
Frozen tissue only
Paraffin + Frozen tissue
Paraffin tissueonly
2008 7 0 6 1
2009 9 2 7 0
• Emphasis on accruing extrapleural pneumonectomy cases
Meso accrual data (cont’d)Meso accrual data (cont’d)
Progression on 5 yr plans-Frozen TMAProgression on 5 yr plans-Frozen TMA
Frozen TMA
Frozen Array mold kit
Frozen TMAFrozen TMA
Normal lung tissue
Tumor tissue (mesothelioma)
MVB #s indicatedistinct patients
N=13 ptsmvb22
21mvb22
21
mvb2189
mvb2215
mvb2219
mvb2189
mvb2215
mvb2219
mvb2190
mvb2191
mvb2197
mvb2190
mvb2191
mvb2197
mvb2217
mvb2214
mvb2199
mvb2217
mvb2214
mvb2199
mvb2203
mvb2211
mvb2213
mvb2203
mvb2211
mvb2213
mvb2212
mvb2212
Frozen TMA: Equipment & AccessoriesFrozen TMA: Equipment & Accessories
• NYU MVB Paraffin TMA 1NYU MVB Paraffin TMA 1• Design Phase:
• N= cases/ Block X 4 B• 4 TMA Blocks (same cases on each
block)• Control tissues, cell lines, frozen-
to-formalin tissue (adv: standardizedprocessing)
• Construction Phase: MTA II• Microtomy: Limited number of sections;
histocollimator• Storage: In desiccators under nitrogen
atmosphere• High resolution scan of slide for website
Plans for Paraffin TMAPlans for Paraffin TMA
Sample paraffin TMA
Paraffin TMA: Equipment & AccessoriesParaffin TMA: Equipment & Accessories
Desiccators
Tissue Micro-Arrayer
Proposed map for Paraffin TMAProposed map for Paraffin TMA
Cell Lines
Tumor
Control
Liver
Column →
Row ↓ 0 1 2 3 4 5 6 7 8 9 10 11 12 13A LiverB C D E F G H I J K L MN O P Q R
Special Thanks to…Special Thanks to…
Today’s conference attendeesToday’s conference attendees
And the entire NMVB teamAnd the entire NMVB team