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3D Surface Inspecto r

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Page 1: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

Page 2: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

Page 3: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

Page 4: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

1. Choose your Method of Image Acquisition.

• Manual acquisition captures a Z-stack within 3DSI• Auto Acq. controls a Prior Scientific™ Z-motor device• Use Scope-Pro to collect Z-stacks in Image-Pro • Import a series of Z interval images

Page 5: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

2. Create EDF and Topographical Map in Focus Settings Tab

Page 6: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

3. Pair EDF and Topographical Map

Page 7: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

4. Measure using 4 different methods

a. Surface Plot b. 3D Profile c. Marker d. Fill Area

Page 8: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

a. Surface Plot

• Visualize the Z Depth• View surface from any angle, using…

– Texture mapping– Wire frame– Pseudo coloring

• Easy rotation of the surface plot rendering• Spatially calibrated axes• Adjustable lighting, contrast and shadow controls for

enhanced viewing. • NEW! Create AVI animations

Page 9: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

Page 10: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

b. 3D Profile

• Maximum and minimum Z values over an X/Y line profile • Z height representation over an X/Y line profile

Page 11: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

b. Live Marker

• X/Y Line Profile on Image

Page 12: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

3D Surface Inspector

c. Fill Area

• Differences in Z height between two X/Y positions • Surface area measurements for object on a focal plane • Measure the volume covered by that surface area

Page 13: 3D Surface Inspector 3D Surface Inspector 1. Choose your Method of Image Acquisition. Manual acquisition captures a Z-stack within 3DSI Auto Acq

Thank You!