6/16/2021 - hhs.uncg.edu
TRANSCRIPT
6/16/2021
Estrogens and Gut Health
CWHW Research Forum
Clinton Allred, [email protected]
Estrogens in Target Organ Systems
Estrogens in Target Organ Systems Exposures
Genistein
Bisphenol A
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Example ProjectXenoestrogens
(BPA, BPS, BPF)Figure 1. Project overview
ER-Dependent Intestinal ResponseColonic epithelia
• Physiological effects• ER signaling and gene
expression
Specific Aim 2
Intestinal Inflammation And Tumor ProgressionSpecific Aim 3
Specific Aim 1
Liver metabolism
DirectResponse
Microbiome• Predictive metabolomics• Bacterial perturbation
Novel microbiotaproduced ER ligands
(i.e. HLT)
Microbiota-modified
metabolites of XEs1 2
Direct Response
AhR-Dependent Intestinal ResponseColonic epithelia
• Physiological effects• AhR signaling and gene
expression
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Crosstalk
AhR ligands/activity
ERligands/activity
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CAPTURING MEASURES OF ENERGY BALANCE AND APPETITE IN HUMAN STUDIES
Dr. Jessica McNeilCWHW Research Forum
University of North Carolina, GreensboroApril 9th 2021
Energy Balance Protocol – In‐Lab Assesments
Arrival
Leave the laboratory
VAST‐0
Anthropometrics and RMR Appetite
Standard Breakfast
VAST‐30
Appetite
VAST‐60
VAST‐90
VAST‐120
VAST‐150
VAST‐180
Thermic Effect of Food Olfactory Sensitivity
Food Reward
Food Reward
Ad libitum Lunch
≈ 6 hours
Energy Balance Protocol – Out‐of‐Lab Assesments
Leave the laboratory
Return to the laboratory
Ad libitum Energy Intake Total Energy Expenditure
≈ 36 hours
Alternative Protocol – In‐Lab AssesmentsArrival
Leave the laboratory
Anthropometrics and RMR
Standard Breakfast
Food Reward
Food Reward
Ad libitum LunchAcute Exercise Interventions
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THANK YOU!
FOLLOW-UP QUESTIONS:[email protected](JESSICA MCNEIL)
Previous Studies
Study Exposures or Interventions Study Outcomes‐Menstrual Cycle
‐Menopausal Transition
‐Acute Exercise Manipulations
‐Acute Dietary Manipulations
‐Acute Sleep Manipulations
‐Exercise and/or Dietary Interventions
‐Energy Intake (1 meal and over 2 days)
‐Energy Expenditure (RMR+TEF+PAEE)
‐Body weight and Body Composition
‐Subjective Appetite Ratings
‐Orexigenic (ghrelin) and Anorexigenic hormone levels (leptin, PYY, CCK)
‐Satiety Quotient (Δmm/kcal)
‐Food “wanting” and “liking”
‐Olfactory Sensitivity
Olfactory Sensitivity
3 TestsOlfactory Threshold
Olfactory DeterminationOlfactory Identification
Pennmemorycenter.org
Food RewardLeeds Food Preference Questionnaire
Finlayson et al., Appetite, 2008, 50(1): 120‐127.
“how much do you want some of this food now?” “How pleasant would it be to experience a mouthful of this food now”
Not at all Extremely
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Ad libitum Energy IntakeFood menu
McNeil et al., Br J Nutr, 2012, 108(7): 1316‐24.
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Food and Brain(FoB Study)
CWHW Research Forum
Steve Fordahl, [email protected]
Jigna Dharod, [email protected]
FoB: Design Aim:Examine the Impact of food availability and stress on brain responses to palatable or healthy foods
n=2018+ yrs old
Receiving Food Assistance
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Home Food EnvironmentFood Inventory
24hr Diet RecallFood Security Survey
Perceived Stress 3‐Factor Eating QuestionnairePower of Food Scale
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MRI x2
Early
Late
FoB: Imaging
Early
Late
Early Benefits Scan• Structural• fMRI (Food Images)• Resting State
Late Benefits Scan• Structural• fMRI (Food Images)• Resting State• DTI
fMRI Blocks (10 images, 3 s each)
Neutral Palatable Neutral Healthy
Block 1 Block 2 Block 3 Block 4
Block 5 Block 6 Block 7 Block 8
Block 9 Block 10 Block 11 Block 12
Block 13
FoB: Future
Target Outcomes• Identify trends in brain activation
• Healthy• Palatable• Healthy > Palatable• Palatable > Healthy
• Identify associations with• Food Inventory• Perceived Stress• Benefit Status
Potential Next Steps• Finish analyzing data
• Resting State & DTI analysis
• Explore relationships• Stress and Future Food Purchases• Does heightened activity in reward centers to palatable food predict future management of food benefits
• Does heightened stress response predict future
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