a novel green biorefinery concept
TRANSCRIPT
![Page 1: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/1.jpg)
A NOVEL GREEN BIOREFINERY CONCEPTMaría Santamaría-Fernández // Section for Sustainable Biotechnology // Aalborg University
![Page 2: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/2.jpg)
2 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
THE ORGANOFINERY CONCEPT – Protein
feed, biogas and ferti l izer
![Page 3: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/3.jpg)
3 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
PROGRESS ALONG THE PROJECT
PROTEIN
BIOREFINING
LAB-SCALE
PROTEIN
EXTRACTION YIELDS
AMINO ACID
COMPOSITION
1
![Page 4: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/4.jpg)
4 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
GREEN JUICE
PRESS CAKE
4
Red clover
Clover grass
Alfalfa
Oilseed radish
Screw pressing
50-60% ww
40-50% ww
Mechanical fractionation of the fresh crops
into green juice and press cake
![Page 5: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/5.jpg)
5 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
GREEN JUICE
5
BROWN JUICE
PROTEIN
CONCENTRATE
Lactic acid fermentation Centrifugation
6-15% ww
Overall, between 3-8% of crops wet weight was
extracted into the protein concentrates
Lactobacillus salivarius BC 1OO1
37°C, overnight
Final pH 4.0-4.7
Lactic acid fermentation of green juices
and production of protein concentrates
![Page 6: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/6.jpg)
6 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
Up to 23% of plant proteins were
recovered in the protein concentrates
23,4
17,1
15,1
12,1
0
5
10
15
20
25
Red clover Clover grass Alfalfa Oilseed radish
Crude protein recovery (%)
PROTEIN CONCENTRATES
![Page 7: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/7.jpg)
7 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
The essential amino acids represented
53% of the true protein content
48%
53%
53%
53%
53%
366
374
346
349
397
0 50 100 150 200 250 300 350 400
Soybeans*
Oilseed radish
Alfalfa
Clover grass
Red clover
Amino acid composition (g kg-1 DM)
Essential AA Non-essential AA
Essential AA for poultry: ARG, HIS, ILE; LEU; LYS, MET, PHE, THR, VAL
*Steenfeldt and Hammershøj, 2015
![Page 8: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/8.jpg)
8 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
3%
5%
4%
4%
4%
174
197
183
185
211
0 25 50 75 100 125 150 175 200 225
Soybeans*
Oilseed radish
Alfalfa
Clover grass
Red clover
Amino acid composition (g kg-1 DM)
Methionine Essential AA
Methionine is usually first- l imiting amino
acid in most poultry feeds
*Steenfeldt and Hammershøj, 2015
![Page 9: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/9.jpg)
9 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
PROGRESS ALONG THE PROJECT
PROTEIN
BIOREFINING
LAB-SCALE
PROTEIN
BIOREFINING
DEMO-SCALE
PROTEIN
EXTRACTION YIELDS
AMINO ACID
COMPOSITION
1SCALABILITY AND
PRACTICALITY
PRODUCE PROTEIN
CONCENTRATE FOR
FEEDING TRIALS
2
![Page 10: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/10.jpg)
Grass clover
feeding system
Screw press
Tank trailer
Fermentation
tank
Press cake,
screw conveyor
![Page 11: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/11.jpg)
10 m3
fermentation tank
Sand
sedimentation tank
Filter sieve
200µm
![Page 12: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/12.jpg)
12 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
Around 10 tons of wet protein concentrate
were produced from 444 tons of clover grass
FRESH CLOVER GRASS
444 tons
PRESS CAKE
223 tons
GREEN JUICE
177 tons
SILAGE BALES
WET PROTEIN CONCENTRATE
7 tons
DRY PROTEIN CONCENTRATE
1 tons
50% ww
40% ww
2% ww
![Page 13: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/13.jpg)
13 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
717
72
67
1621
0
20
40
60
80
100
Grass clover - Demo scale Grass clover - Lab scale*
Crude protein
recovery (%)
Protein concentrate Press cake Brown juice Loss
*Santamaria-Fernandez et al., 2017
The overall protein recovery in the
protein concentrate was 7%
![Page 14: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/14.jpg)
14 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
Content of amino acids was lower than in
protein concentrates produced at lab scale
48%
53%
53%
52%
366
397
349
294
0 50 100 150 200 250 300 350 400
Soybeans**
Red clover -Lab scale*
Grass clover -Lab scale*
Grass clover -Demo scale
Amino acid composition (g kg-1 DM)
Essential AA Non-essential AA
*Santamaria-Fernandez et al., 2017
**Steenfeldt and Hammershøj, 2015
![Page 15: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/15.jpg)
15 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
PROGRESS ALONG THE PROJECT
PROTEIN
BIOREFINING
LAB-SCALE
PROTEIN
BIOREFINING
DEMO-SCALE
PROTEIN
EXTRACTION YIELDS
AMINO ACID
COMPOSITION
1SCALABILITY AND
PRACTICALITY
PRODUCE PROTEIN
CONCENTRATE FOR
FEEDING TRIALS
2PLANT DEVELOPMENT
STAGE ON PROCESS
YIELDS
OPTIMAL HARVESTING
TIME
3
PROTEIN
BIOREFINING
OPTIMIZATION
![Page 16: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/16.jpg)
16 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
Plants were harvested at different development
stages (DS) ensuring morphological changes
Chicory
Cichorium intybus
Red clover
Trifolium pratense
Timothy
Phleum pratense
DS1 ELONGATION
Main shoot
ELONGATION
40% full height
ELONGATION
Second internode
DS2 ELONGATION
5 to 9 internodes
INFLORESCENCE
EMERGENCE
Some buds visible
EARING
70% earing
DS3 INFLORESCENCE
EMERGENCE
First individual flowers (closed)
FLOWERING
Half way to full flowering
![Page 17: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/17.jpg)
17 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
Crude protein content in the plants
significantly decreased with maturity
0
5
10
15
20
25
Chicory Red clover Timothy
Crude protein
content (%DM)
DS1 DS2 DS3
FRESH PLANTS
![Page 18: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/18.jpg)
18 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
Protein extractabil i ty in protein concentrates
decreased significantly with maturity
0
20
40
60
80
100
Chicory Red clover Timothy
Protein
extractability
DS1 DS2 DS3
g CP in Prot conc
kg DM plant
![Page 19: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/19.jpg)
19 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
Production of protein concentrates also
decreased with maturity
0
50
100
150
200
250
Chicory Red clover Timothy
Prot conc.
production
DS1 DS2 DS3
g dry Prot conc
kg DM plant
![Page 20: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/20.jpg)
CONCLUSIONS & FUTURE PERSPECTIVES
• The Organofinery process allowed producing up to 235 kg dry protein concentrate per ton of
dried biomass.
• Laboratory-scale trials:
• Lactic acid fermentation-centrifugation method i.e. between 40-80% of proteins in green
juice can be recovered.
• Up to 42% of proteins in fresh biomass recovered in the protein concentrate.
• Several challenges to overcome in large scale production: separation of proteins and drying of
the protein concentrate.
• Protein concentrate good source of essestial amino acids for poultry.
![Page 21: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/21.jpg)
21 │ MARIA SANTAMARIA-FERNANDEZ // Green Biorefining Seminar // JUNE 29, 2018
FUTURE
PERSPECTIVESFresh
crop
Screw
pressing
Fermen-tation
Protein
separation
Drying
OPTIMIZATION
Food-grade
protein
concentrates?
Techono-
economical
analysis
Alternative
products e.g.
lactic acid
Nutritional value
of protein
concentrates
![Page 22: A NOVEL GREEN BIOREFINERY CONCEPT](https://reader031.vdocument.in/reader031/viewer/2022011908/61d71fd620871322da5735f7/html5/thumbnails/22.jpg)