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JOURNAL OF VIROLOGY

A Versatile Transreplication-Based System To Identify Cellular 

Proteins Involved in Geminivirus Replication

Gabriel Morilla et al.,. Apr 2006, p. 3624±3633

Ori-wt

Replication initiation /

Rep nicking site

Rep binding sites

(2669-2681)

CGC GGTAGCTAGGTAG // 

 A A

T 

 A T 

T 

 A

C C 

T 

 A

G AC T C  AC GGG

C T G AGT GC C C 

T 

Transgenes used in this study

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Detection of episomal replicons

in infected transgenic plantsProbed with 35S Promoter specific probe

Detection of virus

in infected transgenic plantsProbed with TYLCSV and TLCV specific probes

2IRGFP trans Replicons Amplifies During TYLCSV Replication

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Analysis of GFP expression in TYLCSV-infected 2IRGFP plants (3wpi)

TYLCSV infected

wildtype plant

MOCK infected

2IR-GFP plantTYLCSV infected

2IR-GFP plant

TYLCV infected

2IR-GFP plant

TYLCV infected

BINGFP plant

Mock Infected

2IR-GFP plants

TYLCSV Infected

2IR-GFP plants

Roots Stems Buds Flowers

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Analysis of GFP expression in TYLCSV-infected 2IRGFP plants (6wpi)

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Replicon Production and Induction of GFP Expression are

Dependent on TYLCSV Rep.

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Gene Silencing of PCNA Prevents GFP Induction and TYLCSV Replication