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JOURNAL OF VIROLOGY
A Versatile Transreplication-Based System To Identify Cellular
Proteins Involved in Geminivirus Replication
Gabriel Morilla et al.,. Apr 2006, p. 3624±3633
Ori-wt
Replication initiation /
Rep nicking site
Rep binding sites
(2669-2681)
CGC GGTAGCTAGGTAG //
A A
T
A T
T
A
C C
T
A
G AC T C AC GGG
C T G AGT GC C C
T
Transgenes used in this study
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Detection of episomal replicons
in infected transgenic plantsProbed with 35S Promoter specific probe
Detection of virus
in infected transgenic plantsProbed with TYLCSV and TLCV specific probes
2IRGFP trans Replicons Amplifies During TYLCSV Replication
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Analysis of GFP expression in TYLCSV-infected 2IRGFP plants (3wpi)
TYLCSV infected
wildtype plant
MOCK infected
2IR-GFP plantTYLCSV infected
2IR-GFP plant
TYLCV infected
2IR-GFP plant
TYLCV infected
BINGFP plant
Mock Infected
2IR-GFP plants
TYLCSV Infected
2IR-GFP plants
Roots Stems Buds Flowers
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Analysis of GFP expression in TYLCSV-infected 2IRGFP plants (6wpi)
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Replicon Production and Induction of GFP Expression are
Dependent on TYLCSV Rep.
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Gene Silencing of PCNA Prevents GFP Induction and TYLCSV Replication