a215 lab - indiana university bloomingtonanat215/lab/lab_pdfs/intro_cells.pdf · pdfs of a215 lab...
TRANSCRIPT
Introduction/Microscope Intro/Cell Basics/Cell Division
A215 Lab
PowerPoint Introductions
• PDFs of intros can be downloaded from the Lab website’s “Lab Resources” page: http://www.indiana.edu/~anat215/lab/resources.htm
What is Histology?
• Histology=Microscopic Anatomy – Learn what makes up the different tissues
in the body – Learn how different tissues relate to
structure and function (or malfunction)
Types of Microscopy
• Electron Microscopy – Powerful magnifying technique used to
visualize intracellular structures – Up to 105x
Magnification Demo (Pin Head)
x33
Magnification Demo (Pin Head)
x100
Magnification Demo (Pin Head)
x250
Magnification Demo (Pin Head)
x1000
Magnification Demo (Pin Head)
x5000
Magnification Demo (Pin Head)
x30,000
Types of Microscopy • Electron Microscopy
– Powerful magnifying technique used to visualize intracellular structures
– Up to 105x
• Light Microscopy – Thin section of preserved tissue is cut and placed
on a slide and stained – 4x-1000x
• in A215, however, you will magnify at most about 400x (40x magnification of virtual microscope objective lens x 10x lens in eyepiece)
• You need to multiply the number shown on the VM by 10 to find the actual magnification
Types of sectioning
Cross Section Longitudinal Oblique
Muscle
Section? Section? Section?
Cell Basics
Cell Membrane
Nucleus
Cytoplasm
Nucleolus
Cell Basics
• Not all cells look the same
• Cells can vary in terms of visible organelles and shape
PDFs of A215 lab electron micrographs can be downloaded using the link at the bottom of the “Lab Resources” webpage
Cell Organelles
Rough Endoplasmic
Reticulum
Cell Organelles
Mitochondria
Centrioles 9 sets of
triplets
Microvilli
Cilia
Type of sectioning?
Section?
Section?
Cilia 9 doublets
+2
Cell Division
Prophase “Prepare”
Metaphase “Meet”
Anaphase “Apart”
Telophase “Two”
Donor Introduction • Donors are people who freely donated
their bodies for education • Male and Female Donors (no longer
referred to here as cadavers) • New donors re-introduced every Fall • Prosection done by medical students • Remains are cremated and returned to
families • PLEASE SHOW THEM
APPROPRIATE RESPECT
What phase?
What phase?
What phase?
What phase?