academic inventions through multidisciplinary collaborations between weill cornell medical college...
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Academic Inventions through Academic Inventions through Multidisciplinary Collaborations Multidisciplinary Collaborations between Weill Cornell Medical between Weill Cornell Medical College and Ithaca CampusesCollege and Ithaca Campuses
Bo LiuDivision of Vascular Surgery
Translate inventions in material science toTranslate inventions in material science tonovel treatments for vascular diseases.novel treatments for vascular diseases.
Vascular Surgeons
Material Scientists Vascular Biologists
Clinical problems
Experimental hypotheses
Mat
erial
inve
ntion
s
*
HN
HC
O O
NH
H2N NH2
C
O
O O C
OHC
HN
NH
H2N NH2
*x y
PEA polymer
Project I: DNA Carriers Project I: DNA Carriers
Problem: Lack of safe and efficient DNA/RNA carriers for molecular therapy.
Hypothesis: Newly invented PEA polymers are capable of binding DNA and delivering it to vascular cells and tissues.
Cornell University Seed Grant (Liu & Chu)
11/1/06-10/31/07Novel biodegradable biomaterials as non-viral gene transfer systems.
15 μg PEI
Control – no transfectionControl – no transfection
SMCSMC
15 μg PEA 1,500 μg PEA
15 μg Superfect®
CytotoxicityCytotoxicity
unhealthy
healthy
Cytotoxicity: Cell ViabilityCytotoxicity: Cell ViabilityH
igh
er %
, mor
e ce
ll f
rien
dly
Transfection Efficiency in SMCTransfection Efficiency in SMC
Firefly
0.0E+00
5.0E+04
1.0E+051.5E+05
2.0E+05
2.5E+05
3.0E+053.5E+05
4.0E+05
4.5E+05
SF 2-A-6E-Cl-100
2-A-6E-Cl-200
2-A-6E-Cl-500
2-A-6E-Cl-1K 2-A-6E-Cl-2K
RLU/mg
Chu’s PEA samplesCommercial reagent
SMC Transfection by DNA Encoded with SMC Transfection by DNA Encoded with Green Fluorescence ProteinGreen Fluorescence Protein
Outcome: grant proposalsOutcome: grant proposals
NIH R21/R33 submitted on 9/16/07Novel biodegradable polymers as non-viral gene transfer systems.Liu, Kent, Rafii, and Chu
The Uehara Memorial Foundation (Japan)Research Fellowship----- 1/01/08 to 12/31/09Yamanochi
Outcome: publicationOutcome: publication
Transfection of Vascular Smooth Muscle Cells with Novel Biodegradable Arginine Based Poly(ester-amide)s
Dai Yamanouchi, M.D., Ph.D.,1, Jun Wu,2, Andrew Lazar,1, K Craig Kent, MD.,1, Chih-Chang Chu, Ph.D.,2, Bo Liu, Ph.D.1.
1. Weill Medical College; 2. Cornell University
Submitted to Molecular Therapy
Project II: Tissue engineering of blood vesselsProject II: Tissue engineering of blood vessels
Problem: Synthetic arterial grafts fail due to restenosis.
Approach: Using biodegradable materials as scaffold to generate blood vessels ex vivo or in vitro.
Morgan Seed Grant (Chu, Peinhart-King, Liu, and Kent)7/1/07-12/31/08Novel biodegradable scaffolds for tissue engineering of blood vessels.
Chu: Human Ecology
Synthesis of PEA-based biodegradable and biofunctional elastomeric scaffolds
Reinhart-King: BME In vitro
engineering of scaffolds & cell adhesion study using vascular
cells
Liu/Kent: Weill Medical Optimization using
stem-cell mediated healing to enhance success of engineered grafts
Liu/Kent: Weill Medical In vivo animal model &
implantation
Feedback for optimization of design
parameters
Future PlanFuture Plan
NIH: PAR-06-504 (R01)Enabling Technologies for Tissue Engineering and Regenerative Medicine Projected submission date: 9/10/08
Industry grants
Patents
Vascular Surgery LabK. Craig Kent, MDBo Liu, PhD
Rachel EldenBrad Herman, PhDKaori Kato, MDRishi Kundi, MDSebastian Schuble, MDShirling Tsai, MDChunjie WangDai Yamanouchi, MD, PhDFan Zhang, MD, PhDAndrew Zohlman
Department of Surgery
Research and Sponsored ProgramCaren A Heller, MD
Cornell Intercampus Seed GrantMorgan Seed grant
IthacaC.C. Chu, PhD
Hua Song, PhDJun Wu