additional file 4
DESCRIPTION
Additional file 4. Gel images for comparative experiment of two strains to illustrate the process of gel comparisons. Includes comparative gels and images with spots numbered and normalized for GMI1000 (strain A) and P673 (strain B) comparative gels of cell-associated proteins . 225. 150. 102. - PowerPoint PPT PresentationTRANSCRIPT
Additional file 4
Gel images for comparative experiment of two strains to illustrate the process of gel comparisons. Includes comparative gels and images with spots numbered and normalized for GMI1000 (strain A) and P673 (strain B) comparative gels of cell-associated proteins .
MB-09-41-gel 1
5/17/09 2D DIGE. 3-colored image of Ralstonia solanacearum total protein lysate. 100 ug each of Cy3 labeled 18°C-Strain A-1,Cy5 labeled 30°C-Strain A-1, and Cy2-internal standard were mixed and were focused in a 24 cm pH 3 to 11 NL IPG strip at 10000 V for 100 kVHr before analyzing in a 8 to 16% Tris Glycine SDS PAGE
150
102
76
52
38
24
17
225
12
31
Internal standard in blue, A18-1 in green, A30-1 in red
+
MB-09-41-gel 1
150
102
76
52
38
24
17
225
12
31
5/17/09 2D DIGE. 2-colored image of Ralstonia solanacearum total protein lysate. 100 ug each of Cy3 labeled 18°C-Strain A-1,Cy5 labeled 30°C-Strain A-1, and Cy2-internal standard were mixed and were focused in a 24 cm pH 3 to 11 NL IPG strip at 10000 V for 100 kVHr before analyzing in a 8 to 16% Tris Glycine SDS PAGE
+
A18-1 in green, A30-1 in red
102
1055 1083 1124 1150
119
1193
1227
1229
1230 1288
1332 1357 1375
1386
1395
1581 1582
1586 1656
1691
1694
1821
188
243
245 263
279 317
429 456
457 472 483
589 610
616 623 629
630
631
643
68
685 694 703
75 76 81 84
891
A18-1 grey scale image Differentially expressed spots comparison A18-A30
102
1055 1083 1124 1150
119
1193
1227
1229
1230 1288
1332 1357 1375
1386
1395
1581 1582
1586 1656
1691
1694
1821
188
243
245 263
279 317
429 456
457 472 483
589 610
616 623 629
630
631
643
68
685 694 703
75 76 81 84
891
A30-1 grey scale image Differentially expressed spots comparison A18-A30
MB-09-41-gel 2
150
102
76
52
38
24
17
225
12
31
5/17/09 2D DIGE. 3-colored image of Ralstonia solanacearum total protein lysate. 100 ug each of Cy3 labeled 30°C-Strain A-3,Cy5 labeled 18°C-Strain B-2, and Cy2-internal standard were mixed and were focused in a 24 cm pH 3 to 11 NL IPG strip at 10000 V for 100 kVHr before analyzing in a 8 to 16% Tris Glycine SDS PAGE
+
A30-3 in green, B18-2 in red, internal standard in blue
MB-09-41-gel 2
150
102
76
52
38
24
17
225
12
31
5/17/09 2D DIGE. 2-color image of Ralstonia solanacearum total protein lysate. 100 ug each of Cy3 labeled 30°C-Strain A-3,Cy5 labeled 18°C-Strain B-2, and Cy2-internal standard were mixed and were focused in a 24 cm pH 3 to 11 NL IPG strip at 10000 V for 100 kVHr before analyzing in a 8 to 16% Tris Glycine SDS PAGE
+
A30-3 in green, B18-2 in red
MB-09-41-gel 2
150
102
76
52
38
24
17
225
12
31
A30-3 grey scale image+
MB-09-41-gel 2
150
102
76
52
38
24
17
225
12
31
B18-2 grey scale image+
MB-09-41-gel 5
150
102
76
52
38
24
17
225
12
31
5/17/09 2D DIGE. 3-colored image of Ralstonia solanacearum total protein lysate. 100 ug each of Cy3 labeled 30°C-Strain A-3,Cy5 labeled 30°C-Strain B-2, and Cy2-internal standard were mixed and were focused in a 24 cm pH 3 to 11 NL IPG strip at 10000 V for 100 kVHr before analyzing in a 8 to 16% Tris Glycine SDS PAGE
+
A30-3 in green, B30-2 in red, internal standard in blue
MB-09-41-gel 5
150
102
76
52
38
24
17
225
12
31
5/17/09 2D DIGE. 2-colored image of Ralstonia solanacearum total protein lysate. 100 ug each of Cy3 labeled 30°C-Strain A-3,Cy5 labeled 30°C-Strain B-2, and Cy2-internal standard were mixed and were focused in a 24 cm pH 3 to 11 NL IPG strip at 10000 V for 100 kVHr before analyzing in a 8 to 16% Tris Glycine SDS PAGE
+
A30-3 in green, B30-2 in red
MB-09-41-GEL 5
150
102
76
52
38
24
17
225
12
31
Grey scale image of A30-3+
MB-09-41-gel 5
150
102
76
52
38
24
17
225
12
31
Digital image of B30-2+
MB-09-41-gel 7
150
102
76
52
38
24
17
225
12
31
5/17/09 2D DIGE. 3-colored image of Ralstonia solanacearum total protein lysate. 100 ug each of Cy3 labeled 18°C-Strain B-3,Cy5 labeled 30°C-Strain B-3, and Cy2-internal standard were mixed and were focused in a 24 cm pH 3 to 11 NL IPG strip at 10000 V for 100 kVHr before analyzing in a 8 to 16% Tris Glycine SDS PAGE
+
B18-3 in green, B30-3 in red, internal standard in blue
MB-09-41-gel 7
150
102
76
52
38
24
17
225
12
31
5/17/09 2D DIGE. 2-colored image of Ralstonia solanacearum total protein lysate. 100 ug each of Cy3 labeled 18°C-Strain B-3,Cy5 labeled 30°C-Strain B-3, and Cy2-internal standard were mixed and were focused in a 24 cm pH 3 to 11 NL IPG strip at 10000 V for 100 kVHr before analyzing in a 8 to 16% Tris Glycine SDS PAGE
+
B18-3 in green, B30-3 in red
1014
104 105
1060 1076
117
1265 1271 1284
1308 1312 1355 1379 1397 1415 1425 1458 1465
1473 1477 1478
150
1531 1537 1557
1619
1627 1650 1653
1694 1739
174
1748 1769 1795 1798
192 285 377
469 472
475 585
587 602
610 623
656 669 675 703 716 738
82
826 838 875 882 956
99
Digital image of B18-3Differentially expressed spots comparison B18-B30
1014
104 105
1060 1076
117
1265 1271 1284
1308 1312 1355 1379 1397 1415 1425 1458 1465
1473 1477 1478
150
1531 1537 1557
1619
1627 1650 1653
1694 1739
174
1748 1769 1795 1798
192 285 377
469 472
475 585
587 602
610 623
656 669 675 703 716 738
82
826 838 875 882 956
99
Digital image of B30-3 Differentially expressed spots comparison B18-B30