adrian o brien bsc (hons) cv
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Adrian O’Brien BSc Hons
14 Bridewell Place, Collon, Co. Louth.
087 2235875 / 041 9826854
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I currently hold an Honours Degree in General Science, focussing on Biology & Chemistry, and have
gained experience working in a clinical proteomics laboratory. I have experience regarding biomarker
discovery and bioinformatics protocols, on human blood plasma/serum and human saliva biofluid
samples from patients who are, or have previously been, positive for head & neck and colorectal
cancers.
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Skills
I have an excellent overall health and safety profile, aseptic technique, pipetting technique and employ
CGMP/CGLP.
Furthermore, I have experience with cell culture techniques, 1D & 2D SDS PAGE, HPLC, Gram stain,
haemocytometer, light microscopy, protein equilibration using Bradford Assay, optical analysis,
biofluid fractionation & protein enrichment using thermal centrifuge, nanotraps, electrophoresis,
ELISA. I have experience regarding reagent preparation, protein linearisation, trypsin digestion, speed
vacuum operation, sample preparation for mass spectrometry and data analysis utilising various
software packages (proteome discoverer, Max Quant, Scaffold and String). I am computer literate
(ECDL holder) and an experienced Microsoft Office user (WORD, EXCEL & POWERPOINT). I have
an excellent work ethic and work well, either individually or with a team.
I have excellent verbal and written communication skills with excellent telephone etiquette. I am highly
motivated and extremely punctual.
Previous Employment
Employer Maynooth University (Clinical Proteomics Laboratory)
Duration June 2015 – Sept 2015 (Summer Project 3)
Job Description Research Assistant (Fusobacterium nucleatum (FN) abundance and utility in
CRC patients)
Duration August 2014 – Sept 2014 (Summer Project 2)
Job Description Research Assistant (General biomarker discovery for Head & Neck Cancers)
Duration June 2014 – Sept 2014 (Summer Project 1)
Job Description Research Assistant (FN abundance and utility in CRC patients)
During my first summer 2014 project I was involved in the investigation of FN protein abundance in
human blood plasma from patients positive for colorectal cancer (CRC) in comparison with controls.
My responsibilities included thawing human blood plasma samples, vortex, sample preparation,
centrifugation and harvest, protein enrichment through Ceres Nanotrap technology, sample washing
and elution, trypsin digestion, preparation for mass spectrometry, documentation, sample refreezing and
bioinformatics data analysis.
During my second summer 2014 project I was involved in collecting and preparing control saliva
samples for subsequent comparison with samples from patients who were positive for Head & Neck
Cancers. My responsibilities during this project were similar to those mentioned in the paragraph above
with the addition of Griener Bio-one saliva collection protocol utility and test kit validation.
During my summer 2015 project I continued an investigation into the possible utility of FN proteins to
detect the presence of colorectal cancer (CRC) using human blood serum samples.
Employer Department of Defence (Casement Aerodrome)
Duration August 1986 – March 2010
Job Description Radio mechanic, 1st and 2nd line military aircraft communications technician, Search
& Rescue personal locator Beacon Equipment (SARBE) maintenance and repair, Personal Computer
maintenance, upgrades and repair, IT network design, installation and maintenance including
instrument, equipment and ancillary procurement, fibre optic (Singlemode and Multimode) and copper
(Cat 5e & Cat 6) cable installation (internal and external) and termination. My responsibilities also
included administration of switches, routers, VLANs and firewall along with integration of VOIP and
Ericsson telephony systems. I was involved in many line duties including operation of a communication
center (COMCEN), operation of the Ericsson PABX switch and had the responsibility of maintaining
mobile communications during various military exercises.
I have designed and delivered oral presentations on technical matters during my involvement with the
Department of Defence.
Education
University Maynooth University Duration September 2012 – May 2016
Field of Study FETAC Level 8 - Biology (4yrs including modules in Clinical Proteomics,
Immunology (Introduction and Advanced), Medical Mycology, Antibiotics, Control of Protein Activity
and Abundance, Molecular Biology (I&II), Microbiology, Molecular Biology of the Cell, Evolutionary
Biology, Biochemistry and General Biology (I & II))
FETAC Level 7 – Chemistry (3yrs including modules in Organic, Inorganic,
Spectroscopy & Analytical, Heterocyclic & Natural Product and Transition Metal Chemistry)
Mathematics (2yrs including Data Analysis and Statistics) and Experimental Physics (1yr)
I have delivered oral presentations, both individually and as part of a group, during my degree course
on topics such as;
Aphid saliva and the plant-aphid interaction
T-Cell Activation
Differential regulation of IL1 receptor and TLR signalling by MEKK3
College Coláiste Dhúlaigh CFE Duration September 2010 – May 2012
Qualification FETAC Level 6 – Business Management
FETAC Level 5 – Business Studies
Other education achievements and certificates available on request
Volunteer Involvements
Collon Credit Union Ltd. (CCU), Collon, Co. Louth; Chairman March 2005 – August 2010;
I Provided the role of Chairman at meetings for the Board of Directors, Represented CCU at AGM,
Represented the CCU membership at ILCU Conferences, Involvement on Investment, Credit Control
& Credit Committees and acted as CCU Health & Safety Officer
ILCU Chapter 5 (Ch5) (Cavan, Louth and Monaghan);
CCU Delegate December 2007 – August 2010; I represented CCU Directors at Ch5 Delegate meetings
ILCU Ch5 Assistant Chairman January 2009 - January 2010; I assisted the Ch5 Chairman in his duties
and Chaired Ch5 Delegate meetings where required
ILCU Ch5 Secretary January 2010 – March 2010; I provided secretarial duties for ILCU Ch5 and
provided a correspondence conduit between ILCU and ILCU Ch5 Chairman/Treasurer and other ILCU
Ch5 Credit Union membership
Referees & References
Available on request