Advances in High Pressure Bioscienceand Biotechnology II

Springer-Verlag Berlin Heidelberg GmbH

Roland Winter (Editor)

123

Advancesin High PressureBioscienceand Biotechnology II

Proceedings of the 2nd International Conferenceon High Pressure Bioscience and Biotechnology,Dortmund, September 16–19, 2002

Editor

Professor Dr. RoLAND WINTER

University of Dortmund

Department of Chemistry, Physical Chemistry I Otto-Hahn-Str. 6

44227 Dortmund Germany

E-Mail: roland. winter@uni-dortmund.de

ISBN 978-3-642-05674-1 ISBN 978-3-662-05613-4 (eBook) DOI 10.1007/978-3-662-05613-4

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Preface

HPBB 2002 is the second conference of the International Association of High Pressure Bioscience and Biotechnology which was established in 1998. The first conference was held at Kyoto in 2000. The conference in Dortmund provided more than 140 scientists of different disciplines including biochemistry, biophysics, bio-physical chemistry, molecular biology, cell biology, food science, medicine, phar-macology, and engineering with opportunities to present their research as well as exchange the latest information on high pressure bioscience.

The following topics were covered:

High pressure effects on proteins and macromolecules High pressure effects on polyelectrolytes, DNA, RNA High pressure effects on lipids and biomembranes High pressure and enzymatic reactions High pressure microbiology High pressure food science and food technology High pressure applications in bioscience, pharmacy, and medicine High pressure techniques

In fact, for chemists, biochemists, physicists and materials scientists, pres-sure as an experimental variable represents a tool which provides unique informa-tion about the microscopic properties of the materials studied. While for small molecules and simple systems, pressure effects are often small, they can become significant for larger systems, especially for biosystems, which is largely due to the fact that intermolecular non-covalent interactions dominate and determine their structure, dynamics and function. Hence, in many situations pressure is an ideal pa-rameter for reversible changes of biomolecular systems. A particular distinction that sets pressure apart from other perturbants used in biochemistry, such as pH and urea, is that pressure does not introduce new components to the system. Fur-thermore, an advantage over temperature change is that pressure-induced changes are in most cases fully reversible. Certainly one of the most intriguing fields of high pressure studies are un/refolding and misfolding processes of proteins. We are observing that ever-increasing contributions of pressure studies to our fundamental understanding of biological processes also drive high pressure methodologies to the forefront of biophysics and biochemistry.

In addition to its general use as a research tool for investigating the energet-ics, structure, dynamics and the kinetics of transformations of materials at a mo-lecular level, the application of pressure is also being used to modify the properties of materials to preserve or improve their qualities. Hence, interest in high pressure has also become widespread in the field of food science, which has already a long tradition in Japan, and more recently also in pharmacy and medical fields. Mean-while several high pressure techniques are used in the production of industrial products.

This book covers the entire range of current high pressure bioscience and its possible applications. The papers are published as submitted and have not been copy-edited. We hope that this publication will stimulate further developments in basic and applied high-pressure bioscience as well as the cooperation between aca-demia and industry. We would like to express our gratitude to Mrs. B. Gruß, A. Kreusel, and B. Schuppan for their assistance in organizing the conference and preparing the con-ference proceedings.

Dortmund, December 2002 Roland Winter

Local Organizing Committee

R. Winter (Conference Chairman)

G. Holzäpfel, B. Gruß, A. Kreusel, S. Yasui

International Advisory Board

C. Balny, Montpellier; D. H. Bartlett, San Diego; D. Clark, Berkeley; J. C. Cheftel, Montpellier; Y. Taniguchi, Japan; K. Gekko, Hiroshima; R. Hayashi, Kyoto; K. Heremans, Leuven; G. Jenner, Strassbourg; C. Jung, Berlin; C. Kato, Japan; D. Knorr, Berlin; S. Kunugi, Kyoto; N. Klyachko, Moscow; H. Ludwig, Heidelberg; P. Masson, France; C. Royer, Montpellier; J. Silva, Rio de Janeiro; L. Smeller, Buda-pest; R. Vogel, München; B. Tauscher, Karlsruhe

Sponsors

Gesellschaft der Freunde der Universität Dortmund Boehringer Ingelheim GmbH High Pressure Research Center, UnipressEquipment,Warszawa Hi-Tech Ltd., Salisbury

Table of Contents

I Proteins and Macromolecules - Biophysical Aspects

Pressure- and Temperature-induced Phenomena in Proteins K. Heremans 3

High Pressure NMR Spectroscopy Characterizes Higher Energy Conformers of Proteins K. Akasaka 9

High Pressure Raman Spectroscopic and RISM Theoretical Studies of Conformational Equilibria of N-Acetyl-L-Glycine-N’-Methylamide T. Takekiyo, T. Imai, M. Kato and Y. Taniguchi 15

High Pressure as Tool to Study Structural Properties of Thiolate Heme Proteins C. Jung, B. Canny and J.C. Chervin 19

Essential Differences between the Core and Peripheral Antenna Excitons in Photosynthetic Purple Bacteria Revealed by High-Pressure Spectroscopy A. Freiberg, A. Ellervee, A. Lõhmus, R. Lõhmus, K. Timpmann and G. Trinkunas 25

Pressure-induced Unfolding of Myoglobin: Neutron Diffraction and Dynamic Scattering Experiments W. Doster, R. Gebhardt and A.K. Soper 29

Pressure-induced Critical Association of Myoglobin R. Gebhardt, W. Doster, J. Friedrich, W. Petry and A. Schulte 33

Fluorescence Spectroscopy of N-acetyl-L-tryptophan-amide and Proteins under High Pressure: Effects of Pressure, Temperature, and Organic Additives M. Kato, T. Maki and R. Hayashi 37

Conformational Fluctuations of Hen Lysozyme Investigated by High Pressure NMR Spectroscopy Y. O. Kamatari, T. N. Niraula, H. Yamada, C. M. Dobson, L. J. Smith, S. Yokoyama, and K. Akasaka 43

VIII Table of Contents

II Protein Unfolding and Reaction

Pressure-driven Cold Denaturation of Proteins and Structure Formation of Water-soluble Polymers S. Kunugi and N. Tanaka 49

Exploring the Energy Landscape of Protein Unfolding under High Pressure R. Lange, J. Torrent, S. Marchal, M. Vilanova, P. Fusi and C. Balny 55

Double-mutant Thermodynamic Cycles under High Hydrostatic Pressure P. Masson 61

Pressure Unfolded States of Ribonuclease A under Native and Reducing Conditions have Identical Conformations F. Meersman and K. Heremans 69

Hydrostatic Pressure Effects on the Stability of Ataxin-3 S. Marchal, E. Shehi, P. Fusi, P. Tortora and R. Lange 73

Insulin and Polylysine as Model Polypeptides for FTIR Studies of the Pressure-effect on Protein Aggregation W. Dzwolak, M. Kato, S. Porowski and Y. Taniguchi 79

Kinetic Behaviour of Sulfolobus Solfataricus Carboxypeptidase towards Several Substrates Highlights the Effects of Pressure and Temperature on Non-covalent Interactions P. Tortora, B. Gambirasio, N. Bec, E. Occhipinti and R. Lange 83

The Structural Basis for Functionality and Stability of Hydrophobic Core in RNase A as Probed by the Application of High Pressure E. Chatani, T. Kadonosono and R. Hayashi 87

Folding and Unfolding Behavior of Carboxypeptidase Y Inhibitor (IC)Induced by Temperature and Pressure J. Mima, Y. Sato, M. Kato and R. Hayashi 95

High Pressure NMR Spectroscopy and its Application to the Cold Shock Protein TmCsp Derived from the Hyperthermophilic Bacterium Thermotoga maritimaW. Kremer, M.R. Arnold, E. Brunner, B. Schuler, R. Jaenicke and H.R. Kalbitzer 101

Influence of High Pressure on the Secondary Structure of Poly-L-Lysine H. Plangger, M. Scheibenzuber, G. Blümelhuber and R. Meyer-Pittroff 113

Table of Contents IX

Temperature- and Pressure-induced Unfolding of -Chymotrypsin Z. Sun and R. Winter 117

Pressure Effects on Ligand Binding Kinetics and the Heme Environment in Myoglobin A. Schulte and O. Galkin 121

III Proteins and High Pressure Enzymology

Revisiting some Observations Related to the High Pressure Action on Enzyme Reactions M.J. Kornblatt, J A. Kornblatt and C. Balny 127

Pressure as a Tool in the Comparative Studies of Fluorescent Proteins S.A. Smirnov, A.E. Pozhitkov, N.L. Klyachko, P.A. Levashov, V.V. Verkhusha, A.V. Levashov, J.-W. Borst, A. van Hoek and A.J.W.G. Visser 133

IV Protein Misfolding and Aggregation

Interprotein Interactions Perturbed by Pressure. High Pressure Studies on Aggregations of Medical Impact L. Smeller, F. Meersman, J. Fidy and K. Heremans 141

Temperature/Pressure Study of Insulin Fibrillogenesis C. Dirix, I. Esters, F. Meersman and K. Heremans 147

A High-pressure Approach to Examine Novel Prion Conformational Changes J. Torrent, M.T. Alvarez-Martinez, F. Heitz, J.-P. Liautard, C. Balny and R. Lange 151

High Pressure Enhances the Chaperone Activity of the Oligomeric Protein Alpha-Crystallin C. Böde, F. Tölgyesi, L. Smeller, K. Heremans and J. Fidy 155

X Table of Contents

V Protein Crystallography

New Trends in Macromolecular Crystallography at High Hydrostatic Pressure R. Fourme, I. Ascone, R. Kahn, E. Girard, M. Mezouar, T. Lin and J.E. Johnson 161

Effects of Pressure on the Growth Kinetics of Orthorhombic Lysozyme CrystalsY. Suzuki, H. Suto, T. Sawada and K. Tamura 171

Effect of Pressure on the Crystallization of Proteins A. Kadri, C. Charron, M.C. Robert, B. Capelle, G. Jenner, R. Giegé and B. Lorber 175

VI Nucleic Acids

Towards an Understanding of the Mechanism of the Aminoacylation Reaction at High Pressure M. Giel-Pietraszuk, P. Sa a ski, J. Jurczak and J. Barciszewski 181

VII Membranes and Lipid Mesophases

Pressure Effects on Columnar Lyotropics: X-ray Diffraction Investigation on d(GMP) and GMP P. Ausili, M. Pisani, C. Ferrero, S. Finet and P. Mariani 189

Effects of Cis and Trans Unsaturation on the Barotropic and Thermotropic Phase Behavior of Phospholipid Bilayers S. Kaneshina, H. Matsuki, M. Kusube and H. Ichimori 195

Barotropic and Thermotropic Phase Behavior of Bilayer Membranes Composed of Phospholipids Containing Cis Double Bond in the sn-2 Acyl Chain H. Matsuki, M. Kusube, T. Hata and S. Kaneshina 199

Effects of Gas Pressure on the Phase Transition Temperature of Liposomal Bilayer Membranes K. Tamura, T. Matsumoto and Y. Suzuki 203

Table of Contents XI

High-Pressure Stopped-Flow Studies to Characterize Transient Conformational Transitions of a Membrane Enzyme: Na, K-ATPase E. Grell, C. Saudan, P. Bugnon, E. Lewitzki and A.E. Merbach 207

Inhibition of Na+, K+-ATPase by Hydrostatic Pressure S. Janosch, E. Kinne-Saffran, R.K.H. Kinne and R. Winter 215

Hydrostatic Pressure as a Tool to Study F-type ATPsynthases M.O. Souza, T.B. Creczynski-Pasa, H.M. Scofano, P. Gräber and J.A. Mignaco 221

Volume Changes During the Proton Transfer Reactions of Bacteriorhodopsin B. U. Klink, R. Winter, M. Engelhard and I. Chizhov 227

Barotropic Behaviour of Monoolein Cubic Mesophases Embedding Cytochrome c J. Lendermann and R. Winter 231

The Role of Matrix Structure in Pressure Regulation of Enzymes Encapsulated in Ternary Surfactant-Water Organic Solvent Systems N. Klyachko, R. Köhling, J. Woenckhaus, A.K. Kazarov, S. A. Smirnov, A.V. Levashov and R. Winter 235

Activation of Na+ amd L-type Ca2+ Currents in Muscle is More Susceptile to Prolonged High Pressure Treatments than Voltage-dependent Inactivation O. Friedrich, K.R. Kress, H. Ludwig and R.H.A. Fink 241

VIII Microbiology

High Pressure Response of Lactic Acid Bacteria R.F. Vogel, M.A. Ehrmann, M.G. Gänzle, C. Kato, M. Korakli, C.H. Scheyhing, A. Molina-Gutierrez, H.M. Ulmer, R. Winter 249

Baroprotective Effect of Sucrose and NaCl on Lactococcus lactisA. Molina-Gutierrez, M.G. Gänzle and R.F. Vogel 255

Effects of High Pressure on Bacteria and Fungi H. Ludwig 259

The Influence of the Rate of Pressurizing on the Inactivation of Bacillus Subtilis Var. Niger Spores A. Urzica, C. Hölters and H. Ludwig 267

XII Table of Contents

The Role of Tryptophan Permease Tat2 in Cell Growth of Yeast under High-Pressure Condition F. Abe 271

Low Pressure Shock Response of Yeast H. Iwahashi, E. Ishidou, M. Odani, T. Homma and S. Oka 275

Effects of High Pressure and Heat on Muscle Fiber and Connective Tissue of Bovine Skeletal Muscle Rusman, T. Nishium and A. Suzuki 279

High Hydrostatic Pressure (HHP) Inactivation of Five Pharmacopoeia Strains Including Spores of Bacillus Subtilis Y. Rigaldie, A. Largeteau, G. Lemagnen, L. Grislain and G. Demazeau 283

Preliminary Molecular Analysis of an High-Pressure-Modified Type of Bacillus Thuringiensis Ssp. Israelensis, DNA- and Protein-studies. I. Sorokine-Durm, K. Werner, M. Schauer and H. Ludwig 287

IX Biomedical Applications

High Pressure Applications in Medicine R. Meyer-Pittroff 295

High Pressure Treatment of Human Bonetissue - First Investigations about Mechanics and Biology G. Blümelhuber, P. Diehl, S. Fischer, B. Frey, M. Hadaller, S. van Laak,W.Mittelmeier, M. Schmitt, C. Weiss and R. Meyer-Pittroff 307

Apoptosis and Necrosis of Mammalian Cells after High Pressure Treatment - Prospects for a Tumor Vaccine G. Blümelhuber, S. Fischer, U S. Gaipl, M. Herrmann, J.R. Kalden, R E. Voll and R. Meyer-Pittroff 311

Decontamination and Sterilization of Sensitive Drugs using High Hydrostatic Pres-sure (HHP) Y. Rigaldie, A. Largeteau, G. Lemagnen, L. Grislain and G. Demazeau 315

Mechanism of Inactivation of HIV-1 by the Freeze Pressure Generation Method (FPGM) T. Otake, T. Kawahata, H. Mori, Y. Kojima, I. Oishi and K. Hayakawa 321

Table of Contents XIII

X Food Science - Chemical and Technological Aspects

High Pressure - Low Temperature Processing of Foods: A ReviewJ.C. Cheftel, M. Thiebaud and E. Dumay 327

Influence of High Hydrostatic Pressure on the Formation of Non-enzymatic Browning Products Formed in Maillard-type Reactions I. Heberle, P. Schieberle and T. Hofmann 341

Influence of High Hydrostatic Pressure on the Formation of Key Maillard-type Flavour Compounds from D-glucose and L-proline F. Deters, T. Hofmann and P. Schieberle 347

Comparison of Structural Cell Membrane Changes Induced by Different High Pressure Processes at Low Temperatures C. Luscher, O. Schlüter, A. Angersbach and D. Knorr 351

Effects of High-pressure Gas on the Growth Thermograms of Yeast T. Arao, Y. Muramoto, Y. Suzuki and K. Tamura 355

The Influence of High Pressure Treated -Glucan-gel on the Filterability of Beer S. Fischer and R. Meyer-Pittroff 359

Influence of High Pressure Treatment on the Allergenicity of Foods M. Scheibenzuber, V. Grimm, G. Blümelhuber, H. Behrendt, J. Ring and R. Meyer-Pittroff 363

Sterilization by High Hydrostatic Pressure: Increasing Efficiency and Product Quality by Improved Temperature Control W.B.C. de Heij, L.J.M.M. Van Schepdael, R. Moezelaar and R.W. van den Berg 367

High Pressure Freezing Effects on Vegetables A. Largeteau, Y. Lambert, G. Demazeau, N. Moussaoui and J.M. Bouvier 371

Stabilization of Agaricus Bisporus Mushrooms by Combined Action of High Pressure and Heat C. Verret, P. Ballestra, L. Jacquet and A. El Moueffak 375

Effect of High-Pressure on the Denaturation of Meat and Lupin Proteins as Assessed by DSC N. Chapleau, S. Delépine, M.H. Rannou and M. de Lamballerie-Anton 379

XIV Table of Contents

Transcriptome Profile and Cluster Analysis of Saccharomyces Cerevisiaeunder Hydrostatic Pressure ConditionsT. Homma, Y. Momose, E. Ishidou, M. Odani, H. Shimizu, S. Oka and H. Iwahashi 385

High Pressure Inactivation of Yeast and Spheroplasts at Subzero Temperature J.-M. Perrier-Cornet, S. Tapin and P. Gervais 389

Transitiometric In Situ Measurements of Pressure Effects on the Phase Transitions During Starch Gelatinization M. Orlowska, S.L. Randzio and J.-P.E. Grolier 395

Effect of High Pressures on Enzymatic System of Mesophilic Starters Used for Cheesemaking M. Krasowska, A. Reps, A. Jankowska and M. Bielecka 399

Stabilisation of Strawberry Puree Treated by High Pressure During Storage M. Navarro, C. Verret, P. Pardon and A. El Moueffak 403

Determination of the Activation Parameters of the Early Steps of Greening in Dark-grown Wheat Leaf Homogenates by High Pressure Fluorescence Spectroscopy L. Smeller, K. Solymosi, B. Böddi and J. Fidy 407

Biological Sample Preparation System Using Pressure Cycling Technology (PCT)F. Tao, N.P. Lawrence, W.W. Miller, C. Li, P. Tuzmen, J. Behnke, B. Nakhai,A. Kakita, T. Christian, D. Reed, M.M. Manak and R.T. Schumacher 413

Sterilization of Fruit Juice by High Pressure CO2

T. Parton, E. Rocchi, S. Spilimbergo, N. Elvassore and A. Bertucco 419

Freezing Kinetics Due to Ice III Formation in Potato Tissue O. Schlüter, V. Heinz and D. Knorr 425

Effect of Ultra High Pressure Under Argon and Temperature on the Volatiles and Piperine Content and Microbiological Quality of Black Pepper (Piper Nigrum L.) S. Sk pska, B. Windyga, E. Kostrzewa, Z. Jendrzejczak, K. Kar owsk, M. Fonberg-Broczek, H. ciezy ska, A. Grochowska, K. Górecka, S. Porowski, A. Morawski, J. Arabas and S. Szczepek 431

High Pressure Pasteurisation and Sterilisation of Tomato Puree A. M. Matser, B. Krebbers, S.W. Hoogerwerf, R. Moezelaar and R.W. van den Berg 437

Table of Contents XV

High Pressure Calorimetry as a Tool to Monitor Phase Transitions in Foods: Application to Water and Selected Lipids M. Hayert, A. Le Bail, M.H. Rigenbach and E. Gruss 441

A Novel High Pressure Technology for the Production of Margarine Y. Nosho, S. Hashimoto, M. Kato and K. Suzuki 447

On the Influence of High Pressure on Edible Oils S.V Kapranov., M. Pehl, Chr. Hartmann, A. Baars and A. Delgado 453

Pressure Treatment of Food: Instantaneous but not Homogeneous Effect A. Delgado and Chr. Hartmann 459

Modelling and Numerical Simulation of Pressure Induced Phase Changes of a Fluid Food System W. Kowalczyk, Chr. Hartmann and A. Delgado 465

High Pressure Differential Scanning Calorimetry of Proteins: Attenuation of Peak. K. Obuchi 473

Pressure Perturbation Calorimetry H. Heerklotz, R. Winter, C. Royer and J. Seelig 477

On the Calculation of the Compressibility from Ultrasonic Velocity H. Pfeiffer and K. Heremans 481

An Internet-based Possibility for Exchange of Informations between High Pressure Researchers - The Ultra High Pressure Webproject G. Blümelhuber, S. Fischer and R. Meyer-Pittroff 485

Author Index 487