aje best practices workshop usp

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BEST PRACTICES FOR WRITING AND PUBLISHING YOUR RESEARCH Amy Beisel Strategic Partnerships Manager www.aje.com [email protected] © AJE

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Page 1: AJE Best Practices Workshop USP

BEST PRACTICES FOR WRITING AND PUBLISHING YOUR RESEARCHAmy BeiselStrategic Partnerships Manager

www.aje.com

[email protected]

© AJE

Page 2: AJE Best Practices Workshop USP

MORNING SESSION9h30 – 10h: Opening and Scholarly Publishing Report

10h – 12h: Best Practices for Reading and Writing Scholarly Articles

• Keeping up with the literature• Best practices for writing each section:

Introduction, Materials and Methods, Results, Discussion• English editing basics• Avoiding plagiarism

Page 3: AJE Best Practices Workshop USP

AFTERNOON SESSION13h30 – 15h: Best Practices for Reporting Your Research

and Submitting Your Manuscript

• Selecting data and formatting figures• Writing a title and abstract• Choosing a journal• Writing a cover letter

15h20 – 16h20: Roundtable with Experts

• Cases, Questions, and Answers

16h20: Closing Remarks

Page 4: AJE Best Practices Workshop USP

WHO WE ARE

Page 5: AJE Best Practices Workshop USP

WHO WE ARE

We help researchers successfully communicate their work.

Page 6: AJE Best Practices Workshop USP

WHO WE ARE

• PhDs: 100 on staff and 2500 in expert network• Editors recruited from top US graduate programs• Subject-matter experts in 400 areas of study• Native English speakers• 60k manuscripts / year, 400k since 2004• Proprietary software matches manuscripts with

experts• Multilingual support staff• Partnerships with major publishers and societies

Page 7: AJE Best Practices Workshop USP

WHO YOU ARERaise your hand if you are…

• Principle Investigator / Faculty Member

• Post-Doctoral Researcher

• Graduate Student

• Undergraduate Student

Page 8: AJE Best Practices Workshop USP

WHO YOU ARE

Raise your hand if you have ever…• Been an author on a published paper• Written a manuscript• Formatted a manuscript• Created figures for a manuscript• Had a manuscript rejected…

so you had to start all over again!

Page 9: AJE Best Practices Workshop USP

PUBLISHING IS TIME CONSUMING

http://indico.cern.ch/event/332370/session/10/contribution/24

Page 10: AJE Best Practices Workshop USP

arc.aje.com

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THE EXPERTSTheresa Anderson, PhD – Scientific IllustratorAriana Bevilacqua, MS – Editing Team ManagerPaul Klenk, MBA/PhD – Financial AnalystLuciana Leopold, PhD – Editing Team ManagerTony Macias, MA – Academic Translation AdvisorBen Mudrak, PhD – Communications ManagerMichaela Panter, PhD – Academic EditorSilvia Santos, PhD – Translation Team ManagerAshley Smith, PhD – Scientific IllustratorKurt Spurlock, MA – Quality ManagerApril Troester, PhD – Customer Service Director

Page 12: AJE Best Practices Workshop USP

AJE SCHOLARLY PUBLISHING REPORT 2015Trends around the world and in Brazil

Page 13: AJE Best Practices Workshop USP

PUBLISHING TRENDS 2015Publication output is rapidly increasingChina is contributing a larger share every year

Page 14: AJE Best Practices Workshop USP

TEN YEARS IN REVIEW: 2005-2015

84% increase in publications:• >900,000 more in 2015 than 2005

Emerging markets playing a large role:• 204% increase in Brazil’s output• 220% increase in India’s output• 191% increase in China’s output

Medical papers have skyrocketed, with over 266,000 more published in 2015 than in 2005

Page 15: AJE Best Practices Workshop USP

PUBLISHING TRENDS: BRAZILBrazil’s growth has been slower but steady:

>50,000 papers for 2 years running

Page 16: AJE Best Practices Workshop USP

PUBLISHING TRENDS: BRAZIL

www.scimagojr.com

Brazil’s research output has increased relative to Latin America: now the majority

Page 17: AJE Best Practices Workshop USP

PUBLISHING SNAPSHOT: BRAZIL 2015Brazil has an outsize influence on Agricultural fieldsFields represented here?

Page 18: AJE Best Practices Workshop USP

PUBLISHING SNAPSHOT: BRAZIL 2015Universidade de São Paulo was 2nd in the world in terms of publication output by a research institution (after Chinese Academy of Science)

Page 19: AJE Best Practices Workshop USP

SNAPSHOT: BRAZIL 2015

Brazilian researchers published most frequently in

• PLOS ONE • Espacios• Seminas: Ciências Agrárias• Ciência e Saúde Coletiva• Ciência Rural

Page 20: AJE Best Practices Workshop USP

LOOKING BACK: BRAZIL 2005-2015

• Overall, 204% increase in publications (over 26,000 more papers last year than 2015)

• Brazil has nearly doubled its share of publications worldwide, to ~3% of the total

• Brazil has maintained its position as the largest research producer in Latin America

Page 21: AJE Best Practices Workshop USP

BEST PRACTICES FOR READING AND WRITING SCHOLARLY ARTICLES

Page 22: AJE Best Practices Workshop USP

BEST PRACTICES: OVERVIEW

• Keeping up with the literature and evaluating credibility• Writing each section of your manuscript:

Introduction, Materials and Methods, Results, Discussion

• English editing basics• Plagiarism in academic papers

Page 23: AJE Best Practices Workshop USP

KEEPING UP WITH THE LITERATURE

Page 24: AJE Best Practices Workshop USP

KEEPING UP WITH THE LITERATURE

2 million articles28,000 journals

every year

How can I keep up with this amount of material?How can I be sure to read the most relevant articles for my

field?

Page 25: AJE Best Practices Workshop USP

1) LITERATURE-BASED SEARCH ENGINES

These return citation results based on

keywordsauthor names

article titlejournal type

Examples of free search engines:

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PubMed: You can search 26 million biomedical citations from MEDLINE, life sciences journals, and online books. May include links to full-text content.

PubCrawler scans daily updates to the PubMed and GenBank databases and emails you an alert.

PubChase: You can search the biomedical literature, create libraries, and receive recommendations based on saved articles.

Agris is a search engine for agricultural science and technology.

Google Scholar is one of the most widely used academic search engines. It’s good for “free” versions but returns a lot of gray literature or irrelevant results.

Visit Wikipedia’s “List of academic databases and search engines” for an extensive list, including discipline and access cost

Page 27: AJE Best Practices Workshop USP

2) RSS FEEDS

• Allow you to track particular websites by subscribing to automatic updates of web content

• Headlines and summaries / abstracts are listed via a feed reader

• Readers are available as add-ons in internet browsers

• After you download the RSS reader, you can subscribe to the RSS feed via journal webpages

Page 28: AJE Best Practices Workshop USP

3) TABLE OF CONTENT (TOC) ALERTS• Publishers offer TOC alerts for newly published journal

issues• Include Early View and online articles• Sign up at the publisher’s website

• SpringerAlerts, Wiley eTOC, Nature e-alert• JournalTOCs – aggregator for 28k journals including SciELO

Page 29: AJE Best Practices Workshop USP

4) LISTSERVS

• Receive journal TOCs, calls for papers, and dataset announcements via email distribution lists

• Listservs are often managed by universities and scientific organizations

• Search for associations in your discipline and ask if they offer a listserv

Page 30: AJE Best Practices Workshop USP

5) SOCIAL NETWORKS FOR ACADEMICS

Several benefits to engaging on academic networking sites:

• Identify relevant literature• Contact with researchers in your field• Building name recognition in your field• Create opportunities to collaborate

Page 31: AJE Best Practices Workshop USP

SOCIAL NETWORKS FOR ACADEMICS

How many of you have heard of the following services? How many of you use them?

• LinkedIn• Twitter• ResearchGate• Academia.edu• Google Scholar citation profile• ORCID

Page 32: AJE Best Practices Workshop USP

LINKEDIN

• Networking• Online CV• Controlling your image

Page 33: AJE Best Practices Workshop USP

TWITTER

• Instantaneous information

• Trading links• Public Q&A

Page 34: AJE Best Practices Workshop USP

RESEARCHGATE

• Networking• Sharing publications• Q&A with other members

Page 35: AJE Best Practices Workshop USP

ACADEMIA.EDU• Sharing full text• Discovering other articles• Searches that lead to you

Page 36: AJE Best Practices Workshop USP

GOOGLE SCHOLAR PROFILE

• Citations• Alerts for similar

articles• Largely automated

Page 37: AJE Best Practices Workshop USP

DISCUSSION

Turn to the person next to you and share one method that you often use to learn about relevant research articles.

What is a new method that you plan to try?

Any suggestions that we haven’t listed?

Page 38: AJE Best Practices Workshop USP

EVALUATING RESEARCH CREDIBILITY

You’ve read the literature

You’re ready to cite it

But how do you know if it’s credible?

Page 39: AJE Best Practices Workshop USP

COMMON ASSESSMENTS OF CREDIBILITY

1) Journal’s reputation

2) Author’s reputation

3) Number of article citations

Page 40: AJE Best Practices Workshop USP

CAVEAT: JOURNAL REPUTATION

Even if a journal is prominent in your field, peer reviewed, highly selective, and published by a well-regarded publisher, the article may be flawed

Journals with high impact factors have higher retraction rates

High quality research may be published in lesser-known journals, regional journals, specialized journals, or less selective journals

Other caveats?

Page 41: AJE Best Practices Workshop USP

CAVEAT: AUTHOR REPUTATION

An author’s seniority, reputation, publication record, education, affiliation with a prominent institution, or training by a prominent researcher do not guarantee that the article is credible

Why not? Your ideas?

Page 42: AJE Best Practices Workshop USP

CAVEAT: CITATION COUNT

Citation numbers can be inflated or deflated based on the factors we just mentioned:

readers’ perceptions of the journal’s reputation and author’s reputation

Page 43: AJE Best Practices Workshop USP

RISKS OF RELYING ON THESE ASSESSMENTS

1) Citing questionable studiesMay undermine the credibility of your study and perpetuate inaccuracies in the scholarly record

2) Omitting credible studies May weaken your study by overlooking findings that support or refute your work

Page 44: AJE Best Practices Workshop USP

ASSESSING CREDIBILITY

• Was the research design appropriate for the question? Were proper controls used?

• Is the work still relevant? Are there any retraction notices, errata, or negative citations?

• Are the references in the paper credible and properly interpreted?

• Is the reference list comprehensive?• How do the post-publication peer reviews evaluate the

quality of the study? (comments, PubPeer, PubMed Commons, Open Review)

Page 45: AJE Best Practices Workshop USP

ASSESSING CREDIBILITY

Taking a closer look at the research design and context will help you to become more familiar with the literature and more confident in the credibility of your sources.

DISCUSSION:Turn to the person next to you and describe an example of a research article you have read where the journal, author, or citation count was not an accurate indicator of the quality of the research.

Please share an example with the large group.

Page 46: AJE Best Practices Workshop USP

BEST PRACTICES FOR WRITING YOUR MANUSCRIPT

Keeping up with the literatureEvaluating research credibilityWriting Introduction, Materials and Methods, Results, Discussion

Page 47: AJE Best Practices Workshop USP

HOW TO TURN A LAB NOTEBOOK INTO A SCIENTIFIC MANUSCRIPTCarefully maintaining your lab notebook will make writing easier

For all experiments, record the following:• Objective• Materials and methods

• chemical reagents, analytical techniques, theoretical calculations, actual measurements, etc.

• Experimental procedure• Outcome / results• Any notable observations (especially if unexpected)

Do this for the 100th time for every procedure, not just the 1st – you never know which details will be significant

Page 48: AJE Best Practices Workshop USP

CHOOSING THE RIGHT STORY

1) Find all of your data and sort it – select the ones that tell this story

• You can put each experiment on a card or post-it so you can shuffle them around when planning

2) Next, create the figures for each point you want to make

3) Now, think about the narrative that connects each card – this will help you organize your arguments

Let’s look at some tips, section by section

Page 49: AJE Best Practices Workshop USP

ANATOMY OF A SCIENTIFIC RESEARCH ARTICLE1) Introduction2) Materials and Methods3) Results4) Discussion and Conclusions5) References6) Figures7) Title and Abstract

Page 50: AJE Best Practices Workshop USP

Cetin and Hackam (2005) Journal of Surgical Research

Page 51: AJE Best Practices Workshop USP

THE INTRODUCTIONSetting the stage

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PURPOSE OF THE INTRODUCTION

Overview of the manuscript but distinct from the Abstract

• Abstract summarizes the entire paper• Introduction places the results in the context of the

larger field of study

Page 53: AJE Best Practices Workshop USP

QUESTIONS TO GUIDE YOUR INTRODUCTION

What is known about this topic?

What is not yet known?

How does this manuscript add to the body of literature in my field?

What was the purpose or hypothesis of the study?

Page 54: AJE Best Practices Workshop USP

THREE PARTS TO THE INTRODUCTION

1) Overview of previous researchWhat do we know?

2) Description of gaps in the current literatureWhat don’t we know?

3) Summary of the hypothesis underlying the studyWhat does this study contribute?

Page 55: AJE Best Practices Workshop USP

BE GENERAL FIRST, THEN SPECIFIC

“Individuals differ radically from one another in the degree to which they are willing and able to express their emotions.”

“Indeed, the popular view is that such emotional expressiveness is a central difference between men and women.... But the research evidence is mixed...”

“There is even some evidence that men may actually...”

“In this study, we recorded the emotional reactions of both men and women to filmed...”

(Bem 2003)

Page 56: AJE Best Practices Workshop USP

INTRODUCTION TIPSAssume that no one has read the Abstract

Define all abbreviations“Solid-state fermentation (SSF) is defined as the cultivation of microorganisms…”

Define key terms“surface-attached communities termed biofilms, in which the bacterial cells surround themselves with a self-produced matrix of sugar, protein, and DNA”“relative to planktonic (free-living) counterparts”

Connect the Title and Introduction

Page 57: AJE Best Practices Workshop USP

CITING PREVIOUS WORK:STRIKE A BALANCEProvide enough detail to place the results in context

…but not so much that you bore the readerPresent both sides of the previous literature

• Aim for an objective review• If you seem to be hiding some prior results,

reviewers may criticize it• Acknowledge different perspectives

Page 58: AJE Best Practices Workshop USP

CITE RECENT LITERATURERecent = past 5 years in most cases

Pitfalls of older literature:• The paper may have been disproven• The paper may not tell the whole story anymore• Readers may think you are not truly acquainted with the

field

Exception: citing historically important references or theories“Since the isolation of O139 Vibrio cholerae in 1993 [Smith et al.]”

Page 59: AJE Best Practices Workshop USP

HOW MANY REFERENCES?

Include enough references to support your hypothesis

The Introduction does not need to be an exhaustive review

Each reference should support a statement, not simply be an example

Avoid a string of references at the end of one sentence“have been examined using a variety of assays, mutagens, and strain backgrounds (31, 38, 41, 53, 56, 59).”

Many journals will allow the use of (e.g., [31] and [53])

Page 60: AJE Best Practices Workshop USP

NOTE “GAPS” IN THE LITERATURE

Each field has an infinite number of open questions –

mention a few that your manuscript answers

Don’t just mention a gap – explain why anyone should fill it

“Understanding how the P127 oncogene contributes to the transformation of epithelial cells could lead to the discovery of novel anticancer therapeutics.”

Page 61: AJE Best Practices Workshop USP

“GAPS”: OTHER THOUGHTS

If others have mentioned the question you’re answering, cite them:

this supports the importance of your project

Focus on gaps that are well defined“need to cure cancer” too broad“as yet, the portion of lipopolysaccharide bound by heat-labile enterotoxin is unknown” better

Page 62: AJE Best Practices Workshop USP

RECAP YOUR STUDY

Highlight what prompted you to perform the studyConnect your study to the gaps you defined earlier

“Because little is known about the types of V. cholerae that cause disease in non-epidemic settings, we investigated the infectious doses of several non-O1/non-O139 serogroups.”

Page 63: AJE Best Practices Workshop USP

HYPOTHESIS/PROBLEM QUESTIONHypothesis:

Specific prediction for results given current knowledgeProblem question:

Unanswered question or gap that you will fillHypotheses are encouraged

Otherwise, reviewers may think you were “fishing”Use clear, unambiguous language

“If competition lowers reproductive output, then we expect fruit size to be smaller when tree density increases.”

Page 64: AJE Best Practices Workshop USP

SUMMARIZE RESULTS BRIEFLY

Commonly, one sentence for methodsNo numerical data or statistics

“In the present study, a SUP4-o forward-mutation system was used to reexamine the relationship between MMR and Polη in preventing GO-associated mutagenesis in yeast.”

And one sentence for a major conclusion“The results reported here demonstrate that Polη can function independently of MMR to prevent GO-associated mutagenesis, presumably through its ability to bypass these lesions in an error-free manner.”

Page 65: AJE Best Practices Workshop USP

DON’T OVERSELL THE RESULTS

In mathematics proofs are common, but in biology, nothing is “proven”Use words that soften your text

likelyindicatescontributes to our understanding ofsuggestsfurther elucidates

Page 66: AJE Best Practices Workshop USP

INTRODUCTION: SUMMARY

Introduction provides context for your results:why did you write the paper?

Three parts:• Overview of results of previous studies• Description of key “gaps”• Summary of the current hypothesis and results

Be sure to include a hypothesis or problem question

Page 67: AJE Best Practices Workshop USP

INTRODUCTION: SUMMARYWhen your Introduction is written, ask yourself:

Have I described the context for my work and created a convincing narrative using references?

Your reader will look for an answer to the question:Why did the authors write this paper?

Page 68: AJE Best Practices Workshop USP

INTRODUCTION: CHECKLIST

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MATERIALS AND METHODSWhat did you do? How did you do it?

Page 70: AJE Best Practices Workshop USP

WHY SPEND TIME ON THE MATERIALS AND METHODS SECTION?

The ultimate form of peer review is reproduction of published results

The importance of the results cannot be interpreted if the study methods are unclear

A good Methods section leads to reproducibility and credibility

Page 71: AJE Best Practices Workshop USP

WHY SPEND TIME ON THE MATERIALS AND METHODS SECTION?To keep the manuscript organized:

this is the framework for the Results sectionTo avoid rejection

“One of the more common reasons for rejection of a manuscript is that the reviewers cannot fully understand how the study was conducted.” Provenzale (2007) American Journal of Roentgenology

Other reasons?

Page 72: AJE Best Practices Workshop USP

WHEN IS IT BEST TO WRITE THE M&M?

While you are performing your experiments:

• Record key details

• Perform important controls

• Plan the next experiments logically

• Save time when you start writing manuscript(s)

Page 73: AJE Best Practices Workshop USP

PROPER ORDER

Order is important

Organize the section on two levels:Basic characteristics of the entire study … leading to details of individual experimentsMatch the order of methods and results reported

Page 74: AJE Best Practices Workshop USP

OVERALL FRAMEWORK

Study subjects • Human subjects, animals, bacterial strains, etc.• Approval and ethical statements

Interventions (for clinical trials)Experimental detailsData analysis

• Statistical methods

Page 75: AJE Best Practices Workshop USP

STUDY SUBJECTS AND ETHICSProvide all relevant information about study subjects

Humans: age, sex, how they were recruited, inclusion/exclusion criteria, medical conditions, control groups, sample size Animals: species, breed, housing/feeding, sample sizeMicroorganisms: species, strain, serotype

“Institutional review boards at the University of Manitoba in Winnipeg, Canada and St. John’s Medical College and Hospital in Bangalore, India approved the study and the verbal consent process. ”

Page 76: AJE Best Practices Workshop USP

INTERVENTIONS

For clinical trials or studies involving animals

• Drugs, hormones, antibiotics used (with manufacturer)• Method of administration (dose, route)

For studies involving microorganisms, list antibiotics used to maintain cultures

“All strains containing the inducible plasmid construct were grown in Luria-Bertani broth supplemented with ampicillin (100 µg/ml).”

Page 77: AJE Best Practices Workshop USP

EXPERIMENTAL DETAILSUsing the past tense, describe how each experiment was carried out and how data were measured

• Report methods in the same order as the corresponding results

• Make sure all results have methods described

• When using methods that are described in a previous paper, include a proper citation

• When modifying previous methods, include a citation and fully describe the changes

Page 78: AJE Best Practices Workshop USP

EXPERIMENTAL DETAILS WITH CITATIONS

Total sugar content was measured by a colorimetric method using phenol and sulfuric acid according to Taylor (1995).

“using the method of Taylor et al. (1995)”“as previously described (Taylor, 1995)”“using a previously described method (Taylor, 1995)”

“HESN status in the sexually exposed Colombian cohort was defined as previously described [9].”

Page 79: AJE Best Practices Workshop USP

PROVIDE SUFFICIENT DETAILS

Some terms (e.g., “analyzed”) can be vague – always state exactly what you measured or assessed

“Each fraction was analyzed for oxygen content”“Each fraction was assessed for the presence of oxygen”“The oxygen content in each fraction was measured”

Don’t confuse what was measured with what was performed“Cd binding capacity was performed”“Cd binding capacity was measured”“Measurement of Cd binding capacity was performed”

Page 80: AJE Best Practices Workshop USP

STATISTICAL ANALYSIS

Fully describe all statistical tests“An independent, two-tailed Student’s t-test for equal variance was used to compare the branch length in each group.”

If more than one test was used, specify when and how you decided to use one or the other

“Competition indices with a normal distribution, as determined by the Kolmogorov-Smirnov test, were tested for differences by Student’s t-test. Data sets without a normal distribution were tested for differences using the Mann-Whitney U test.”

Define the threshold for statistical significance“p-values < 0.05 were considered statistically significant.”

Page 81: AJE Best Practices Workshop USP

Materials and MethodsThis section is a common cause of rejection for lack of detail

Important questions:• Have I explained my methods sufficiently so that they can be reproduced

by others?

• Do I cite all previously described methods?

• Do I have too much text?

• Do I have the appropriate controls?

• Did my choice of methods introduce any bias into the results?

• Have I chosen the correct techniques to address my research question, and used the correct statistical analyses?

Page 82: AJE Best Practices Workshop USP

Materials and Methods: Final Considerations

Check journal requirements:• Animal studies (ARRIVE guidelines, IACUC approval)• Human subjects (CONSORT guidelines, IRB

approval) • Source of reagents

Consider: Do the results of the methods I chose answer my hypothesis?

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RESULTS, DISCUSSION, CONCLUSIONClosing the case

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RESULTS: KEY COMPONENTS

• The results should relate to the study objective

• Tie experiments together“Having shown that salamanders prefer cool water over warm water, we next determined whether water was preferred to other liquids.”

• Highlight important data, but include anything relevantPresent p-valuesTrim data that don’t fit your objective

• Follow the most logical order Not necessarily chronologicalReread for any gaps

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DISCUSSION AND CONCLUSIONSLabel the final paragraph Conclusions

• Highlight major findings• Restate the study’s importance• Clarify the “takeaway message”

Address limitations• Balance with strengths• Don’t dwell on limitations that are outside of your control• End on a positive note

Page 86: AJE Best Practices Workshop USP

DISCUSSION AND CONCLUSION: FINAL CONSIDERATIONS

Base your conclusions only on the data• What do the results allow you to conclude?• Assumptions about what might be happening should be

clearly presented as speculationRemember that…

If your experimental design doesn’t answer your objective…If you generalize your results too far…If your conclusions are not supported by your data……the journal editor will not consider the work publishable

Page 87: AJE Best Practices Workshop USP

ENGLISH EDITING TIPS

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BE CONCISEReaders focus on beginnings and ends

Longer sentences = larger “forgotten” middle

Rutgers University

Page 89: AJE Best Practices Workshop USP

LENGTHY SENTENCES

Take a moment to read this sentence:“On the tops of crinoids is a circle of plates called radials, which in some primitive crinoids is further divided into a lower inferradial and an upper superradial, and below the radials is a circlet of plates called basals.”

39 words(Carpenter, 2001)

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LENGTHY SENTENCES

Now read the following:“On the tops of crinoids is a circle of plates called radials. In some primitive crinoids, these radials are divided into lower inferradials and upper superradials. Below the radials is a circlet of plates called basals.”

12, 14, and 13 words

(Carpenter, 2001)

Page 91: AJE Best Practices Workshop USP

LENGTHY SENTENCES

Long introductory dependent clause

Because…Because only certain proteins are transported into the nucleus of the cell, and many such proteins contain nuclear localization signals (NLSs) that are recognized by importins, we searched the primary sequences of the two novel proteins for homology to canonical NLSs.

Only certain proteins are transported into the nucleus of the cell, and many such proteins contain nuclear localization signals (NLSs) that are recognized by importins. We therefore searched the primary sequences of the two novel proteins for homology to canonical NLSs.

40 words

24 and 16 words

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LENGTHY SENTENCES

Flesch reading ease and Flesch-Kincaid grade level scores can be used to objectively evaluate readability.

Flesch reading easy guidelines: - 90.0-100.0 Easily understood by an 11-year-old student - 60.0-70.0 Easily understood by 13- to 15-year-old students - 0.0-30.0 Best understood by university students

https://en.wikipedia.org/wiki/Flesch%E2%80%93Kincaid_readability_tests#cite_note-7

Page 93: AJE Best Practices Workshop USP

CUT OUT EXTRANEOUS WORDS

Short, clear sentences are easier to digest and make the point stronger

As seenCurrentlyBecauseToWhether

Shorter versionLengthy versionAs can be seenAt the present timeDue to the fact thatIn order toWhether or not

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REDUNDANT PHRASES

Avoid extra words that add no meaning

Absolutely essential essentialAggregate together aggregateCompletely filled filledEnd result resultFewer in number fewerThroughout the entire study throughout the study

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FORMAL TONEScientific writing does not really involve English as we normally speak it

Jargon (transfect)

Specialized meanings (transform)

Unique conventions (gender vs. sex)

Deviating from this tone will stand out to reviewers and readers –

It’s important to maintain formality

Page 96: AJE Best Practices Workshop USP

FORMAL TONE – THINGS TO AVOID

Possessive formsThe material’s melting point was…The melting point of the material was…

ContractionsCan’t Isn’tIt’s Haven’t

“Ghost quotes”Nicknames (e.g., calling oncogenes “time bombs”)Quotation marks are appropriate for direct quotes.

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FORMAL SYNONYMS

AddressesApproximatelyLargeCurrentlyPerformed/conductedSuch asObserved

Formal synonymInformal termDeals withAbout/aroundBigNowadaysDoneLikeSeen/saw

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WORDS WITH LITTLE MEANINGHas/is

Cd nephrotoxicity is tubular dysfunctionCd nephrotoxicity manifests as tubular dysfunction

The species has three toxinsThe species secretes three toxins

InterestingOne of its interesting functions is…One of its relevant functions is… One unique function of this protein is…

Page 99: AJE Best Practices Workshop USP

WORDS WITH LITTLE MEANINGImportant

Rice is one of the most important cropsRice is one of the most commonly consumed crops OR Rice is a critical food source for billions

SuperiorThis method is superior to previous protocolsThis method is faster than previous protocols ORThis method requires less starting material than previous protocols

Page 100: AJE Best Practices Workshop USP

PRONOUN USAGEAvoid ambiguity with demonstrative pronouns

This, that, these, those

These correspond to…These features of the cells correspond to…

And other pronounsIt was not active in the absence of Mg2+.The enzyme was not active in the absence of Mg2+.

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PRONOUN USAGE

Who vs. thatUse ‘who’ to introduce information about human beingsUse ‘that’ or ‘which’ to introduce information about inanimate objects or other nouns

Patients who presented with three of the following symptoms were included.The final two subjects, who were recruited from the same site, both tested negative.

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PRONOUN USAGE

That vs. which

‘That’ introduces essential information“The car that is parked next to mine has a bird painted on it.” “The cells that were transfected glowed green.”

‘Which’ introduces additional nonessential information“Uruguay, which won the first FIFA world cup, will play against Venezuela later this summer.”“Vibrio cholerae, which causes the deadly diarrheal disease cholera, is endemic to many tropical regions.”

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ANTHROPOMORPHISM

Human emotions or behaviors projected onto other animals or to inanimate objects

It is unclear why cows in the US choose to face north when they eat.It is unclear why cows in the US only eat when facing north.Bacteria in rich soil regularly attack each other.Bacteria in rich soil frequently secrete compounds that kill neighboring bacteria.

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MISPLACED MODIFIERSPut explanatory phrases near what they modify

The patient experienced severe pain in his right arm when lifting objects for three months.

For three months, the patient experienced severe pain in his right arm upon lifting objects.

Copper enters the environment in high concentrations due to agricultural activity in the form of copper sulfate.

Page 105: AJE Best Practices Workshop USP

PARALLEL STRUCTUREMatch elements in a series so that they serve the same grammatical purpose

WordsPhrasesClausesSentences

• Possible therapeutic approaches include surgery, undergoing radiation therapy, and to give chemotherapy.

• Better: Possible therapeutic approaches include surgery, radiation therapy, and chemotherapy.

noun

passive voice/ gerund

infinitive

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AVOIDING PLAGIARISMGiving credit where it is due

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WHAT IS PLAGIARISM?

U.S. Office of Research Integrity defines plagiarism as “the appropriation of another person’s ideas, processes, results, or words without giving appropriate credit”

Academic misconduct that can result in• Diminished credibility• Rejection / retraction from a journal• Dismissal from a university or research

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MAIN TYPES OF PLAGIARISM

Verbatim plagiarism:

Copying text word for word from someone else’s work without citing the source

Plagiarism of ideas:

Mentioning someone else’s unique idea – theory, interpretation, data, method, opinion, or new terminology – without citing the source

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OTHER TYPES OF PLAGIARISMLoose paraphrasingParaphrasing someone else’s work with only slight changes, maintaining the author’s logic and ideas, without citing the source

Plagiarism from alternate sourcesFailing to cite the source of publicly available knowledge, such as websites, blogs, lectures

Self-plagiarism and duplicate publicationRecycling your own previously published text, whether a paragraph or a whole article

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WHY PLAGIARIZE?Discussion: turn to the person next to you and discuss why someone may purposely or accidentally plagiarize

Your thoughts?

Some possibilities:• Pressure to publish• Desire to advance in career• Anxiety about writing original work in English• Struggle to express complex ideas in their own words• Carelessness while writing• Heavy reliance on few sources• Belief that “imitation is the sincerest form of flattery”• Lack of understanding of norms around plagiarism

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HOW TO AVOID PLAGIARISM IN YOUR WRITING

1) As you read the literature, keep careful records of your sources

Citation software can help: Zotero, ReadCube, EndNote

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HOW TO AVOID PLAGIARISM IN YOUR WRITING2) Draw on multiple sources to ensure content diversity, and err on the side of citation

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3) After writing, review your manuscript and reference list to ensure that all appropriate citations are included

Plagiarism software can help: Turnitin, iThenticate, eTBLAST (free)

HOW TO AVOID PLAGIARISM IN YOUR WRITING

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WHEN TO CITE

When including verbatim text• Also place it inside quotation marks

When paraphrasing unique ideas, logic, information – regardless of the source

When mentioning your previously published work

When reproducing / adapting others’ graphs or tables • Request permission first

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WHEN NOT TO CITE

When describing your own work for the first time

When mentioning common knowledge• Information in general reference texts• If you are not sure, cite it

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MORNING SESSION9h30 – 10h: Opening and Scholarly Publishing Report

10h – 12h: Best Practices for Reading and Writing Scholarly Articles

• Keeping up with the literature• Best practices for writing each section:

Introduction, Materials and Methods, Results, Discussion

• English editing basics• Avoiding plagiarism in academic papers

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QUESTIONS / DISCUSSION

BREAK

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AFTERNOON SESSION13h30 – 15h: Best Practices for Reporting Your Research

and Submitting Your Manuscript

• Data and figures• Finishing touches: title and abstract• Choosing a journal• Writing a cover letter

15h20 – 16h20: Roundtable with Experts: Cases, Questions, and Answers

16h20: Closing, Questions, and Final Observations

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BEST PRACTICES FOR REPORTING YOUR RESEARCH

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DATA AND FIGURES

Ashley Smith, PhDScientific Illustrator

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AFTERNOON SESSION13h30 – 15h: Best Practices for Reporting Your Research

and Submitting Your Manuscript

• Selecting data and formatting figures• Writing a title and abstract• Choosing a journal• Writing a cover letter

15h20 – 16h20: Roundtable with Experts

• Cases, Questions, and Answers

16h20: Closing Remarks

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FINISHING TOUCHESTips for writing a strong title and abstract

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CHOOSING A TITLE

Most important part of the paper• Many readers will only see the title• First chance to make a good impression

How do I write a good, appropriate title?

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KEEP IT SHORT…

Lengthy titles will not be completely read• Research shows that papers with shorter titles are viewed

and cited more frequently (Paiva et al., 2012)

• 16 words is sufficient (Knight, 1996)

Remove filler terms• ‘effects of’• ‘comparison of’• ‘a case of’/ ‘a study on’

Do not use abbreviations to save space

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…BUT DON’T MAKE IT TOO SHORT

Too little information means readers will skip your paper

• They may not know it is relevant to them• “Novel cancer biomarker” is short but not

informativeInclude enough information to make your study unique

“Compensatory evolution of net-charge in influenza A virus hemagglutinin”

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AVOID QUESTIONS AND LENGTHY CONSTRUCTIONS• Choose a descriptive phrase – no filler words• Questions do not make good titles for scientific manuscripts

• You should be providing an answer

• Complete sentences often add excess words

“Red hens undergo spontaneous chromosome rearrangement when exposed to ultraviolet light” vs.“Ultraviolet light-induced chromosome rearrangement in red hens”

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DON’T OVERSELL THE MANUSCRIPT

Avoid bold terms unless they are absolutely true – reviewers will be skeptical

• “novel”• “first time”

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TITLE TIPS

Make sure your paper relates to your title

“Molecular mechanism of chromosome rearrangement in red hens” should provide a full mechanism

You may need to temper the language: “Protein X contributes to chromosome rearrangement in red hens”

Only mention methods if they are the paper’s focusPapers with methods-based titles are read less frequently (Paiva et al., 2012)

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KEYWORDS

Use keywords that a reader would search for• Organism name• Most common name for a gene• Name of key technique

Place most important terms at the beginning and end

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TITLE: FINAL THOUGHTS

Get your colleagues’ help!• Offer three or four choices and see which grabs

their attention

Reevaluate the title after any revisions or resubmissions

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THE ABSTRACT: FIRST AND LAST

The Abstract is found at the beginning…but write it last

Always select a journal first• Word count• Differences in format and other

requirements

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WRITING THE ABSTRACT

Summarize key points from every section• Reason for the study• Hypothesis• Primary methods used• Key results• Importance of the study

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WRITING THE ABSTRACT: WHAT TO AVOID

Never refer to the text• “will be discussed further in the text”

Avoid citations if at all possible• Citations clutter the abstract and draw attention away

from your workLeave out numbers and statistics

• Focus on conclusions from data/overall resultsMinimize abbreviations

• But always define the ones you use

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UNNECESSARY INFORMATION

Make every word count:• Remember to stay on topic. Avoid mentioning

interesting findings or experiments if they aren’t relevant to this story.

• Choose effective verbs• Critically examine each word/phrase: does it

convey important information?

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TELLING READERS THEY ARE READING A PAPER

Is it necessary to remind readers that you are describing your results?

We found that neither group showed a statistically significant increase over the course of the experiment.Our results indicate that protein X is not functional in the absence of magnesium.

Note: this is okay when contrasting with others’ findings in the Introduction and Conclusion

XX

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TELLING READERS THEY ARE IN THE PRESENT

Is it necessary to remind your readers that you are describing current knowledge?

In the present day, numerous archaeal genomes have been fully sequenced.There are currently known to be seven components of the ATC complex.

Note: this is okay if you are directly contrasting the findings with what was previously thought

XX

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EXAMPLES OF WORDINESS

There were several subjects who completed…

Several subjects completed…

Copper, which is one of the most harmful heavy metals,

Copper, one of the most harmful heavy metals,

X

X

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CHECKLIST – TITLE AND ABSTRACT

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SAMPLE ABSTRACT

180 words

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REVISED ABSTRACT

136 words

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SAMPLE ABSTRACT

Original:At this time, very little is known about the family of proteins called editins.

More concise version:Little is known about the editin family of proteins.

14 words

9 words

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SAMPLE ABSTRACT

Original:While it has previously been shown that editins can promote the progression of the cell cycle in various cell types, the exact role that this family of proteins plays is not clear.

More concise version:While editins promote cell cycle progression in various cell types, the exact role they play is unknown.

33 words

17 words

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SAMPLE ABSTRACT

Original:In this study, we isolated two editin proteins, EDT1 and EDT2, from the yeast Saccharomyces cerevisiae and characterized some basic biochemical properties, such as molecular weight, isoelectric point, and multimerization status.

More concise version:Here, we isolated two editins, EDT1 and EDT2, from the yeast Saccharomyces cerevisiae and characterized several basic biochemical properties…

32 words

28 words

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SAMPLE ABSTRACT

Original:It was found that both editins were present as dimers.

More concise version:Both editins were found as dimers.

10 words

6 words

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SAMPLE ABSTRACT

Original:EDT1 as a monomer is 52 kDa with a pI of 5.4, and EDT2 as a monomer is 54 kDa with a pI of 5.7.

More concise version:Monomeric EDT1 is 52 kDa with a pI of 5.4, and monomeric EDT2 is 54 kDa with a pI of 5.7.

25 words

21 words

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SAMPLE ABSTRACT

Original:Epitope tagging studies further demonstrated that heterodimers of EDT1 and EDT2 could also be isolated, but it is unknown whether or not heterodimers are functionally equivalent to homodimers of EDT1 or EDT2.

More concise version:Epitope tagging demonstrated that heterodimers of EDT1 and EDT2 also exist, but it is unknown whether heterodimers are functionally equivalent to EDT1 or EDT2 homodimers.

32 words

21 words

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SAMPLE ABSTRACT

Original:Here, we describe the basic properties of two editins from Saccharomyces cerevisiae as well as a protocol for their purification.

More concise version:We report the basic properties of two yeast editins and a protocol for their purification.

21 words

15 words

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SAMPLE ABSTRACT

Original:Additional future studies will be needed to determine how these editins contribute to cell cycle progression.

More concise version:Future studies will be needed to determine how these editins contribute to cell cycle progression.

16 words

15 words

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CHOOSING THE BEST JOURNALFOR YOUR PAPER

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THE GOAL: PUBLISH QUICKLY

Publication – still the primary goal of most researchers in an academic setting

Investigate

Present

Review

Publish

Read

Teach

Mentor

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THE GOAL: PUBLISH QUICKLY

Every time you get rejected, it can cost you months of time or more

Find a journal that fits your work closely – take your best shot first

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SCHOLARLY JOURNALSGlobal market:

2,000 journal publishersOver 30,000 active, peer-reviewed scholarly journals

At least 10,000 open access journals

English-language journals:680 publishers11,550 journalsAnnual revenues of $9 to $10 billion

Growing 7-8% per yearThomson Reuters; Directory of Open Access Journals (www.doaj.org)

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PUBLICATION TRENDS

Over 1.5 million articles a year are published Growing at 3% annually (for 200 years!)

Many more are submitted to journals each year

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IDENTIFYING YOUR TARGET JOURNALS

1) Find articles similar to the one you want to publish2) Collect a list of journals that publish those articles3) Research those journals4) Make a prioritized list of target journals

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EXAMPLE SEARCH ENGINES

jane.biosemantics.orgwww.ncbi.nlm.nih.gov/pubmed

scholar.google.com www.journalguide.com

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SEARCH

Use your draft title and abstract (finding a journal)

Use keywords (more general searches)

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PROS AND CONS

PubMed All peer-reviewed Advanced search capabilities Restricted to certain

biomedical journals

Google Scholar Good at finding free versions Expansive Includes gray literature and

junk

JANE Easy interface

Layered over Medline, so no extra coverage

JournalGuide Standardized layout for finding

and comparing journals Coverage beyond PubMed while

still restricted to peer-reviewed literature

Still growing and expanding functions

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ADVANCED SEARCH STRATEGIES

Use “advanced search” options if availableLimit your search to the years 2011-2016

(expand to earlier articles only if needed)Include recent articles or articles in pressNote the name of the publisher to investigate later

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SAVE YOUR SEARCHES TO RUN LATER!

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COLLECT “HITS” INTO JOURNAL LIST

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DEALING WITH THE RESULTS

• A good number of results is 100 – 300If you recover < 30 or > 300 results, expand or restrict your search• Make a list of your top journal choices• Find out more about these journals

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INFORMATION TO GATHER

Find the journal’s website, and pull out key pieces of information:

• ISSN (unique identifier for every journal)• Publisher/affiliated societies• Contact information (in case of questions)• Aims and scope• Publication frequency

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INFORMATION TO GATHER

Look for additional information that can help you make a more informed decision:

• Acceptance rate• Speed

Time to first decision, time to publication online• Costs

Page fees, publication fees, color image fees• Open access policies

Is self-archiving allowed?Is full open access available?

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JOURNALGUIDE.COMFind most of the information you need in one place with a standardized layout:

• ISSN• Publisher/affiliated

societies• Aims and scope• Types of submissions

accepted• Indexing• Acceptance rate• Publication

frequency• Contact info

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JOURNALGUIDE.COMFind most of the information you need in one place with a standardized layout:

• Time to publication/first response

• Submission/page fees

• Color image fees• Open Access

policy

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COLLEAGUES’ EXPERIENCE

One of the most valuable pieces of data about a journal is the experience of real researchers. Ask around!

Advisor/committeeLabmates (past and present)Collaborators

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BENEFITS AND RISKS

High impact factor = stronger “stamp of approval”

High risk of rejection and lost time

Is it more important to get this research out quickly or to maximize the prestige of the journal?

Is this project a primary focus of your lab’s efforts or is it a side project that might be nice to wrap up?

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BENEFITS AND RISKS

Multidisciplinary journal = more readers, broader impact

Need to rewrite paper to appeal to readers with less context and background

Is your work relevant to a broad audience or will its most interested readers be within your field?

Can you easily contextualize your research for a multidisciplinary audience?

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BENEFITS AND RISKS

Specialized journal with fewer submissions = higher chance of acceptance

Risk of “walling off” your research within your field

Is getting the research accepted quickly an important consideration?

Will a specialized journal be visible enough to researchers in other fields?

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BENEFITS AND RISKS

Open access journal = greater exposure and (perhaps) more citations

May not be the most prestigious journal in your field

Chance of running into questionable publishers

Is open access an important motivation for you?

Will you be concerned if only researchers with subscriptions can see your work?

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SOUND RESEARCH “MEGAJOURNALS”

What is a megajournal?Review for soundness of research results and interpretation, not perceived importance/impactBroad subject scopePublishes any and all articles that meet criteria

PLOS ONE

2007 2013Binfield, 2013

All megajournals

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MEGAJOURNALS Increasing number of journals focusing on rigor of research, not perceived

interested/novelty Usually include broad subject areas

Fast and often simpler route to publication

More effort on your part in other areas: Polishing the language Sharing the paper post-publication Demonstrating the value of the paper without relying on the journal

“brand”

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THE FINAL DECISION

Weigh the pros and cons for each journal, then make an ordered list

Start with journal #1 and move down the list only if needed

Remember that the best fit is not the only thing that affects your paper’s chances:

• Edit your paper carefully• Spend time creating strong figures• Write an effective cover letter

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WRITING A COMPELLINGCOVER LETTERMake your case to the journal editor

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TAKE CARE WITH THE COVER LETTER

• The cover letter is your opportunity to convince the journal editor to send your manuscript out for peer review

• It highlights your most important findings

• It explains why your manuscript is a good fit for the journal

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COVER LETTER: INTRO

1) Address the journal editor formally by name, if possible

2) Include your contact information

This information may also appear in the journal’s online submission system, but it is appropriate to include in the letter

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COVER LETTER: OPENING PARAGRAPHS

1) State the manuscript title and author names

2) Mention what type of article it is: research article, review, case study, etc.

3) Describe your study’s rationale and major findings

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COVER LETTER: MIDDLE PARAGRAPH

1) Briefly explain how your manuscript fits the journal’s Aims & Scope

2) Don’t simply state the the findings are “novel” or “of interest to the field – include specific aspects relevant to the journal

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COVER LETTER: FINAL PARAGRAPH

Close with a brief paragraph indicating the following:

1) The manuscript is original work2) No part of the manuscript has been published before3) The manuscript is not under consideration at any other

journal4) There are no conflicts of interest to disclose5) A list of potential reviewers – only if journal requests this6) Any researchers who should not review your manuscript

due to potential conflicts / bias

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COVER LETTER: CLOSING THOUGHTS

A well-written cover letter assures the journal editor that • your manuscript should be considered• it is a good fit for the journal

Good research may be reviewed regardless of the cover letter’s quality, but a great cover letter can help your manuscript stand out

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EXPERT ROUNDTABLEQuestions & answers with AJE’s team of experts in the United States

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arc.aje.com

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THANK YOU AND BEST WISHESFROM THE AJE TEAM

Flavia Jaszczak [email protected] [email protected]

© AJE