ambr clone selection powered by umetrics software for ...€¦ · 7 ivcd qp reference cs1-2 raw...

ambr® clone selection powered by Umetrics®

Software for Consistent Screening and Ranking


Project 1

CRITERIA FILTER CLONE RANKING (22) CLONE/VARIABLE PLOT

RAW DATA - VCD [E5/ML]

GroupImported order

BACK

Viability [%] (Last)

50

Maximise 1 (High)

88.89

Product [g/L] (Last)

Distance

0.9881

Maximise 1 (High)

5.4204

Maximise 1 (High)

3.88982035.3733

VCD [ES/mL] (Integral)

381.4787

Target 1 (High)

965.7720 1550.065

Glucose [g/L] (Last)

0

Maximise 1 (High)

6.35 12.7

Lactate [g/L] (Last)

0.05

Maximise 1 (High)

1.81199 3.574

Qualified (21)

CS1-11 0.188

Reference

CS1-1

CS1-12 0.232

CS1-7 0.233

CS1-8 0.265

CS1-6 0.293

CS2-2 0.295

CS1-2 0.314

CS1-10 0.315

CS2-8 0.325

CS2-12 0.353

CS1-5 0.366

CS2-9 0.367

CS2-7 0.380

CS2-6 0.396

CS2-1 0.455

CS1-3 0.262

Viability [%] (Last) Product [g/L] (Last) VCD [ES/mL] (Integral)Distance Glucose [g/L] (Last) Lactate g/L (Last)

VCO [ES/mL]

0

200

150

100

50

0

2 4 6 8 10 12


Automated Screening and Ranking with Confidence

ambr® clone selection is a new standalone software application that complements the experiments conducted with ambr® systems. It simplifies the workflow for cell line screening and ranking.

Users define selection criteria such as cell density, product titer and product quality attributes and assign priority weightings in order to screen and rank clones. The application uses a unique multivariable desirability assessment for clone ranking.

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• Automation of the clone selection process improves speed and consistency

Valuable scientist time is freed up leading to more accurate analyses

• Selection criteria profiles may be stored and shared within the same server

This means they are accessible to other users in the team and can be applied to new data sets for consistent selection

• A report is generated to record the selection criteria and details of the selected clone candidates

This makes it possible to view and understand the selection comprehensively

• Maximizes use of data from ambr® experiments It can be mined to the full to extract many key insights

• Re-evaluate at any time If selection criteria change during your project, it is fast and

straightforward to run the calculation again

• ambr® clone selection is flexible The application can be used with data from ambr® 15 and

ambr® 250 high throughput for cell | strain, media and feed screening applications

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ambr® clone selectionpowered by Umetrics®

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ambr® clone selectionAutomated Screening and Ranking Accelerating Research with Umetrics® Expertise

Ranking areaA target proximity value is shown next to each clone that represents the distance from the ideal target. Clones are ranked in order according to their proximity (distance) values.

Clones to report onTo make a final selection of clones for the report, check the box next to the clone.

Filter areaSelection criteria are defined here as chosen values of key attributes

The sliders are easily positioned to give an exact set of parameters which will provide the basis for the statistical analysis

Define objective. Should the variable be- high - maximized- low - minimized- or meet a specific target?


Project 1



GroupImported order

BACK


50

Maximise 1 (High)

88.89


Distance

0.9881

Maximise 1 (High)

5.4204

Maximise 1 (High)

3.88982035.3733


381.4787

Target 1 (High)

965.7720 1550.065


0

Maximise 1 (High)

6.35 12.7


0.05

Maximise 1 (High)

1.81199 3.574

Quali�ed (21)

CS1-11 0.188

Reference

CS1-1

CS1-12 0.232

CS1-7 0.233

CS1-8 0.265

CS1-6 0.293

CS2-2 0.295

CS1-2 0.314

CS1-10 0.315

CS2-8 0.325

CS2-12 0.353

CS1-5 0.366

CS2-9 0.367

CS2-7 0.380

CS2-6 0.396

CS2-1 0.455

CS1-3 0.262


VCD [E5/mL]

0

200

150

100

50

0

2 4 6 8 10 12

Priority weightingsUser assigns these for each selection criteria that will be used to determine the ranking order of the clones.

5

Raw data graphDepiction of the raw data profile for selected variable and highlighted clone


Project 1



GroupImported order

BACK


50

Maximise 1 (High)

88.89


Distance

0.9881

Maximise 1 (High)

5.4204

Maximise 1 (High)

3.88982035.3733


381.4787

Target 1 (High)

965.7720 1550.065


0

Maximise 1 (High)

6.35 12.7


0.05

Maximise 1 (High)

1.81199 3.574

Quali�ed (21)

CS1-11 0.188

Reference

CS1-1

CS1-12 0.232

CS1-7 0.233

CS1-8 0.265

CS1-6 0.293

CS2-2 0.295

CS1-2 0.314

CS1-10 0.315

CS2-8 0.325

CS2-12 0.353

CS1-5 0.366

CS2-9 0.367

CS2-7 0.380

CS2-6 0.396

CS2-1 0.455

CS1-3 0.262


VCD [E5/mL]

0

200

150

100

50

0

2 4 6 8 10 12

Clone variable graphThe maximum, minimum, last or integral values of the chosen variables are viewed alongside each other and can be compared to a similarity variable (reference), if used.

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Process and Quality Data Views

Raw data plot This shows all included clones and their process trend over time (x-axis)

Upload process and | or quality data for investigation.Timely resolved process data relates to measurements repeated several times during the process run. Quality data is gained from single time points measured during or after experiment completion.

IVCD

Qp

Reference

CS1-2


50

100

150

200


Project 1

Variable selection areaList of all selected process variables. Check box to include variable in the clone selection process.

Clones includedSelect a clone here to highlight in the raw data plot.

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IVCD

Qp

Reference

CS1-2


50

100

150

200


Project 1

A multivariate similarity variable (such as a glycan profile) can be created and used for comparison as a reference. This feature allows you to calculate similarity of your chosen clone to a reference according to at least 5 selected variables.

Target proximity (distance) represents similarity to the reference in relation to selected variables. The smaller the target proximity value, the more similar the clone is to the reference.

TBD3

TBD4

Multivariate (1)

Reference

Included (21)

CS2-2

CS2-4

CS2-5

CS2-6

CS2-7

RAW DATA - G2F

0

-5

-10


Project 1

Test

CREATE MULTIVARIATE VARIABLE

Clones and reference includedAs on process data view. Here the user can set a reference clone as indicated by the star to be used in the selection process

Raw data plotDisplays the data for all clones in the selected variable.

Clone variable plotDisplays distribution of each quality variable and the profile for each selected clone.

Variable selection areaList of all quality variables. Check box to include variable in the clone selection process

Reporting Function

Select the candidate clones that you want in your report. A printable report (or save as pdf) from ambr clone selection software application will include:

• Summaryplotofselectedcandidatesandanyreferences

• Tableofselectedcandidatesandanyreferences

• Asummaryofthefiltercriteriaused

• Aprojectsummary,listingtheprofileused,thenamesoftheprocess and quality data files uploaded and number ofcandidates and variables used


Project Report

SELECTED CANDIDATES

APPLIED FILTERS

PROJECT SUMMARY

Project summary

Clone name Qualified

YesCS1-1

CS1-8

CS1-9

CS1-6

CS2-8

CS2-2

Yes

Yes

Yes

Yes

Yes 6

5

4

3

2

1 617.75

965.16

707.95

822.02

965.42

990.26

1550.063

69.445

5.420

35.373

0.000

0.000

0.000

0.000

0.000

0.000

0.000

0.000

<= 0.000

<= 0.000

<= 0.000

<= 0.000

<= 0.000

<= 0.000

<= 0.000

<= 0.000

<= 3.890

<= 0.958

50.000-88.890

<= 381.479 0.4

0.2

0.8

1.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

63.46

Objective

Maximize

Maximize

Maximize

Target

Target Range Weight

69.46

69.94

50.00

64.89

61.57 2.78

4.81

4.79

4.03

3.21

5.42 11.63

17.26

21.00

29.18

20.46

21.21

Rank VCD[ES/m/L] (Integral)

VCD[ES/m/L] (Integral)

Name



Product [g/L] (Max)

Product [g/L] (Max)

Distance

Process data Qualitydata Matching clones

Qualitydata Matching ClonesProcess data

Variables20

Clone screening data format.xlsx Clone screening quality data format.xlsx 22/24

Man

Go

GOf

G1

G1

GOf

G1

G1

Distance

SELECTED CANDIDATES

ManDistanceProduct [g/L] (Max)Viability [%] (Last)VCD[E5/m/L] (Integral) Go GOf G1 G1

CS1-6CS1-8CS1-9CS2-2CS2-8CS1-1

PROJECTS Data management Process data Quality data Clone selection Report

Select filter profile �

Test

Untitled Project

PRINT BACK

Nor

mal

ised

dat

a Reference

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Technical Specification

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Minimum server computer specification requirements:

Aspect Requirement Comment

Operating system Windows Professional 7, 8 or 10 (64 bit)

Processor Intel i5-2400 or better (4 cores, 3.10 GHz clock, 6MB cache)

Anti-virus software The customer is responsible for installing any anti-virus software required on the computer

Power No special requirements - domestic socket suitable

Memory 8 GB or more

Hard-disk 500 GB or more; 7200 rpm or more

Display Intel HD Graphics 2000 or better

Minimum screen resolution should be 1280 by 1024 for optimal display

Typical level of graphics found on a standard PC

Network connections Required for remote server application i.e. if server and client are not on the same machine

Any interface inside a corporate network is the customer’s responsibility. Server does not require remote internet access to run in a closed network

Minimum client computer specification requirements:

Aspect Requirement Comment

Supported browser to run software application

Google Chrome >70, Microsoft Internet Explorer 11

Operating system Windows Professional 7, 8 or 10 (64 bit)

Processor Intel i5-2400 or better (4 cores, 3.10 GHz clock, 6MB cache)

Anti-virus software The customer is responsible for installing any anti-virus software required on the computer

Power No special requirements - domestic socket suitable

Memory 2 GB or more

Hard-disk 500 GB or more; 7200 rpm or more Client data storage is negligible

Display Intel HD Graphics 2000 or better

Minimum screen resolution should be 1280 by 1024 for optimal display

Typical level of graphics found on a standard PC

Network connections Required for remote server application i.e. if server and client are not on thesame machine

Any interface inside a corporate network is the customer’s responsibility. Server does not require remote internet access to run in a closed network

The ambr® clone selection application is a client-server solution. The client and server software can be run on the same computer to allow clients local server access. For remote server access, clients must have direct network connection to the server installation/computer.

Each licence of the ambr® clone selection software permits up to 10 concurrent users (up to 10 log in accounts to be set up by the customer administrator). More than one licence can be purchased and installed within the same server if access for more than 10 users is required.

Only a remote server installation can enable the concurrent client usage of this software application. If client and server are run on the same computer, only a single client is able to access the software application.

It is the customer’s responsibility to supply the server computer, the client computer and to perform the installation of the software.

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Scalability

Single-Use from Cell Line Selection and Process Development to Production Scale

• Geometrical similarity of vessel design from ambr® 250 highthroughput upwards

• Consistent mixing and gassing strategies

• Reliable single-use platform

• Perfusion | perfusion mimic capability

• Microcarrier and specialist mixing at low stirring speed capability

ambr® 250 high throughput

BIOSTAT® BUniVessel® SU 2L


Process DevelopmentClone Selection

Media and Process Optimization

BIOSTAT STR® 50

Predictive

Similar Geometry and Sensors - scaling up from 0.025 L to 1000 L

ambr® 15 cell culture


Project 1



GroupImported order

BACK


50

Maximise 1 (High)

88.89


Distance

0.9881

Maximise 1 (High)

5.4204

Maximise 1 (High)

3.88982035.3733


381.4787

Target 1 (High)

965.7720 1550.065


0

Maximise 1 (High)

6.35 12.7


0.05

Maximise 1 (High)

1.81199 3.574

Qualified (21)

CS1-11 0.188

Reference

CS1-1

CS1-12 0.232

CS1-7 0.233

CS1-8 0.265

CS1-6 0.293

CS2-2 0.295

CS1-2 0.314

CS1-10 0.315

CS2-8 0.325

CS2-12 0.353

CS1-5 0.366

CS2-9 0.367

CS2-7 0.380

CS2-6 0.396

CS2-1 0.455

CS1-3 0.262


VCO [ES/mL]

0

200

150

100

50

0

2 4 6 8 10 12

Identification of Critical Process Parameters, Design of Experiment (DOE) and Process Optimization

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Production

BIOSTAT STR® 200 BIOSTAT STR® 500 BIOSTAT STR® 1000 BIOSTAT STR® 2000

Data Acquisition, Monitoring and Control of Bioprocesses

Real-Time Multivariate Statistical Process Monitoring, Cultivation Monitoring with BioPAT® SpectroReal-Time Release Testing

Multivariate Data Analysis of Large Process Data Sets to Identify Key Trends, Correlations and Troubleshoot

Scalable

Similar Geometry and Sensors - scaling up from 0.025 L to 1000 L

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Sales and Service ContactsFor further contacts, visit www.sartorius-stedim.com

www.sartorius-stedim.com

EuropeGermanySartorius Stedim Biotech GmbHAugust-Spindler-Strasse 1137079 Goettingen

Phone +49.551.308.0Fax +49.551.308.3289

Sartorius Stedim Systems GmbHRobert-Bosch-Strasse 5 – 734302 Guxhagen

Phone +49.5665.407.0Fax +49.5665.407.2200

FranceSartorius Stedim FMT S.A.S.ZI des PaludsAvenue de Jouques – CS 9105113781 Aubagne Cedex

Phone +33.442.845600Fax +33.442.845619

Sartorius Stedim France SASZI des PaludsAvenue de Jouques – CS 7105813781 Aubagne Cedex

Phone +33.442.845600Fax +33.442.846545

AustriaSartorius Stedim Austria GmbHModecenterstrasse 221030 Vienna

Phone +43.1.7965763.18Fax +43.1.796576344

BelgiumSartorius Stedim Belgium N.V.Rue Colonel Bourg 1051030 Bruxelles

Phone +32.2.756.06.80Fax +32.2.756.06.81

HungarySartorius Stedim Hungária Kft.Kagyló u. 52092 Budakeszi

Phone +36.23.457.227Fax +36.23.457.147

ItalySartorius Stedim Italy S.p.A.Via dell’Antella, 76/A50012 Antella-Bagno a Ripoli (FI)

Phone +39.055.63.40.41Fax +39.055.63.40.526

NetherlandsSartorius Stedim Netherlands B.V.

Phone +31.30.60.25.080Fax [email protected]

PolandSartorius Stedim Poland Sp. z o.o.ul. Wrzesinska 7062-025 Kostrzyn

Phone +48.61.647.38.40Fax +48.61.879.25.04

Russian FederationLLC “Sartorius Stedim RUS”Uralskaya str. 4, Lit. B199155 St. Petersburg

Phone +7.812.327.53.27Fax +7.812.327.53.23

SpainSartorius Stedim Spain, S.A.U.Avda. de la Industria, 32Edificio PAYMA28108 Alcobendas (Madrid)

Phone +34.902.110.935Fax +34.91.358.96.23

SwitzerlandSartorius Stedim Switzerland AGRingstrasse 24 a8317 Tagelswangen

Phone +41.52.354.36.36Fax +41.52.354.36.46

U.K.Sartorius Stedim UK Ltd.Longmead Business CentreBlenheim Road, EpsomSurrey KT19 9 QQ

Phone +44.1372.737159Fax +44.1372.726171

Sartorius Stedim Biotech UKformerly TAP BiosystemsYork Way, Royston,Herts, SG8 5WY

Phone +44.1763.227200Fax [email protected]@sartorius.com

UkraineLLS “Sartorius RUS”Post Box 440 “B”01001 Kiev, Ukraine

Phone +380.44.411.4918Fax +380.50.623.3162

AmericaUSASartorius Stedim North America Inc.5 Orville Drive, Suite 200Bohemia, NY 11716

Toll-Free +1.800.368.7178Fax +1.631.254.4253

ArgentinaSartorius Argentina S.A.Int. A. Ávalos 4251B1605ECS MunroBuenos Aires

Phone +54.11.4721.0505Fax +54.11.4762.2333

BrazilSartorius do Brasil LtdaAvenida Senador Vergueiro 2962São Bernardo do CampoCEP 09600-000 - SP- Brasil

Phone +55.11.4362.8900Fax +55.11.4362.8901

MexicoSartorius de México, S.A. de C.V.Libramiento Norte de Tepotzotlan s/n,Colonia Barrio Tlacateco,Municipio de Tepotzotlan,Estado de México,C.P. 54605

Phone +52.55.5562.1102Fax [email protected]

PeruSartorius Peru S.A.C.Av. Emilio Cavenecia 264 San Isidro15073 Lima, Perú

Phone +51.1.441 0158Fax +51.1.422 6100

Asia|PacificAustraliaSartorius Stedim Australia Pty. Ltd.Unit 5, 7-11 Rodeo DriveDandenong South Vic 3175

Phone +61.3.8762.1800Fax +61.3.8762.1828

ChinaSartorius Stedim Biotech (Beijing) Co. Ltd.No. 33 Yu’an RoadAirport Industrial Park Zone BShunyi District, Beijing 101300

Phone +86.10.80426516Fax +86.10.80426580

Sartorius Stedim (Shanghai)Trading Co., Ltd.3rd Floor, North Wing, Tower 1No. 4560 Jinke RoadZhangjiang Hi-Tech ParkPudong DistrictShanghai 201210, P.R. China

Phone +86.21.6878.2300Fax +86.21.6878.2882

Sartorius Stedim Biotech (Beijing) Co. Ltd.Guangzhou Representative OfficeUnit K, Building 23Huihua Commerce & Trade BuildingNo. 80 Xianlie Middle RoadGuangzhou 510070

Phone +86.20.37618687 | 37618651Fax +86.20.37619051

IndiaSartorius Stedim India Pvt. Ltd.#69/2-69/3, NH 48, JakkasandraNelamangala Tq562 123 Bangalore, India

Phone +91.80.4350.5250Fax +91.80.4350.5253

JapanSartorius Stedim Japan K.K.4th Fl., Daiwa Shinagawa North Bldg.8-11, Kita-Shinagawa 1-chomeShinagawa-ku, Tokyo, 140-0001 Japan

Phone +81.3.4331.4300Fax +81.3.4331.4301

MalaysiaSartorius Stedim Malaysia Sdn. Bhd.Lot L3-E-3B, Enterprise 4Technology Park MalaysiaBukit Jalil57000 Kuala Lumpur, Malaysia

Phone +60.3.8996.0622Fax +60.3.8996.0755

SingaporeSartorius Stedim Singapore Pte. Ltd.1 Science Park Road,The Capricorn, #05-08A,Singapore Science Park IISingapore 117528

Phone +65.6872.3966Fax +65.6778.2494

South KoreaSartorius Korea Biotech Co., Ltd.8th Floor, Solid Space B/D,PanGyoYeok-Ro 220, BunDang-GuSeongNam-Si, GyeongGi-Do, 463-400

Phone +82.31.622.5700Fax +82.31.622.5799

ambr clone selection powered by umetrics software for ...€¦ · 7 ivcd qp reference cs1-2 raw...

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