07-p023 gudmap – an online genitourinary resource
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suggest that SOX3-expressing cells within the CNS control pitui-
tary endocrine cell size and consequently hormone secretion.
doi:10.1016/j.mod.2009.06.304
07-P022
Applications of lentiviral transgenesis in the chick
Feifei Song, Adrian Sherman, Isabelle Worthington, Mike
McGrew, Helen Sang
Roslin Institute, University of Edinburgh, Edinburgh, United Kingdom
We have previously shown that lentiviral vectors can be used
to generate transgenic chickens efficiently and that expression of
introduced transgenes is not silenced on transmission through
the germline. Transgene expression can be targeted in the pre-
dicted tissue-restricted manner, for example: muscle-specific
expression is restricted to skeletal muscle using the rat myosin
light chain 3 gene enhancer/promoter and oviduct-specific
expression is demonstrated using regulatory elements of the
chicken ovalbumin gene. We also produced transgenic chicken
lines expressing GFP ubiquitously using the CMV IE enhancer
fused to the chicken b-actin promoter/intron. High levels of GFP
protein are visible at all stages of developing transgenic embryos.
GFP embryos have been wildly used in cell-fate experiments
where the grafted cells can be visualised and followed during
embryo development. Here we are extending the repertoire of
reporter genes with the development of transgenic lines that
ubiquitously express membrane-localized GFP or monomeric-
Cherry (red), which will make invivo tracing cell shape changes
and cell interactions possible. Founder males chimeric for trans-
genes coding for membrane-localized fluorescent proteins have
been raised to sexual maturity and crossed to screen for trans-
genic offspring. In addition, we are generating transgenic lines
carry photo-activatable GFP or photo-switchable CFP that should
allow marking of cells insitu for lineage tracing studies without
the need for grafting.
doi:10.1016/j.mod.2009.06.305
07-P023
GUDMAP – An online genitourinary resource
Simon Harding1, Jamie Davies2, Jane Armstrong2, Jane Brennan2,
Sue Lloyd-MacGlip2, Derek Houghton1, Mehran Sharghi1, Xingjun
Pi1, Ying Cheng1, Bruce Aronow3, Sean Grimmond4, Peter
Koopman4, James Lessard3, Melissa Little4, Andy McMahon5,
Cathy Mendelsohn6, Steve Potter3, Michelle Southard-Smith7,
Duncan Davidson1
1MRC Human Genetics Unit, Edinburgh, United Kingdom2University of Edinburgh, Edinburgh, United Kingdom3Cinncinati Children’s Hospital Medical Center, Cincinnati, OH, United
States4University of Queensland, Brisbane, Australia5Harvard University, Cambridge, MA, United States6Columbia University Medical Center, New York, NY, United States7Vanderbilt University Medical Center, Nashville, TN, United States
The GenitoUrinary Development Molecular Anatomy Project
(GUDMAP) is a consortium of laboratories working to provide
the scientific and medical community with gene expression data
and tools to facilitate research (see www.gudmap.org). The data
provided by GUDMAP includes large insitu hybridization screens
(wholemount and section) and expression microarray analysis
of components of the developing mouse urogenital system
(including laser-captured material and FACS-isolated cells from
transgenic reporter mice). In addition, a high-resolution anatomy
ontology has been developed by members of the GUDMAP consor-
tium to describe the subcompartments of the developing murine
genitourinary tract.
The GUDMAP Database Development and Editorial teams,
both based in Edinburgh, function to ensure submission, cura-
tion, storage and presentation of the data submitted by the GUD-
MAP consortium. Thus far, we have developed a range of tools
that help both the submitter and the end user. These include:
an online annotation tool that simplifies insitu data submission
through an ontology-based graphical user interface; a database
interface that allows users to browse and query expression data,
and to filter data by organ system; a heat-map display of micro-
array data and analyses. Furthermore, the Edinburgh team has
developed a GUDMAP Disease Database that queries associations
between genes, genitourinary diseases, and renal/urinary and
reproductive phenotypes.
By virtue of its impressive, high-resolution dataset and its ease
of use we hope that the GUDMAP Website will continue to serve
as a powerful resource for biologists, clinicians and bioinformati-
cians with an interest in the urogenital system.
doi:10.1016/j.mod.2009.06.306
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