2nd lab depex reviewer
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9 Jan 2014
BIO LAB 2nd DepEx Reviewer
I. EXERCISE 8: Longitudinal section of the external morphology of a tadpole
• organogenesis - differentiation of MESODERM
• Schumway's chart of frog development
• 4-7 mm frog embryo : somites, gills, and tail
• 10 mm tadpole: small fish with a prominent tail
- gills
- ventral suckers
- horny jaws
- lips and papillae
- sense organs
- myotomes
II. EXERCISE 9: Serial Transverse section on the development of 10 mm tadpole
A. Level of the Olfactory Placode
• internal nares = choanae
• external nares = olfactory pits
• lateral appendix = outgrowth near epidermis and external nares
• notochord: surrounded by FIBROUS SHEATH
• parachordal cartilage: basophilic nuclei of chondrocytes
• dorsal lip: 3 rows of teeth (3-D)
• venral lip: 4 rows of DECIDUOUS teeth (V-4)
• Layers of the brain wall
-EPENDYMAL LAYER: supportive in function; w/ cilia To move CSF
- MANTLE LAYER: becomes GRAY MATTER of CNS
- MARGINAL LAYER: becomes WHITE MATTER of CNS
B. Level of the Optic Cup
• mandibular muscles vs mandibular cartilage: muscles - fusiform (spindle-shaped) nuclei + greater staining affinity (darker)
C. Level of the Heart <3
• Velar plate: prevents clogging of gill slits with ingested food
• gill rakers: prevent passage of solid particles through the gill slits; mechanical sifting of water
• internal gills: derived from branchial arches III, IV, and V
• GILL FILAMENTS
D. Level of the Glottis
E. Level of the lung buds
• nephrostome: lined by cilia; removal of larval waste products is accomplished by CILIARY MOTION
• spiracle: for egress of water
F. Level of the mesonephros
G. Level of the cloaca
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22 Jan 2014
I. 18-hour chick embryo whole mount
*notochord (still a head process!)
*HEAD ECTODERM
II. SERIAL TRANSVERSE SECTION OF AN 18-HOUR CHICK EMBRYO
A. LEVEL OF THE NOTOCHORD OR HEAD PROCESS
*notochord/head process
B. LEVEL OF THE HENSEN'S NODE
C. LEVEL OF THE PRIMITIVE PIT
D. LEVEL OF THE PRIMITIVE GROOVE
*PRIMITIVE GUT
*AREA PELLUCIDA
*AREA OPACA
III. WHOLE MOUNT OF A 24-HOUR CHICK EMBRYO
*SUBCEPHALIC POCKET
*MARGIN OF THE FOREGUT
*NOTOCHORD
IV. SERIAL TRANSVERSE SECTION OF A 24-HOUR CHICK EMBRYO
A. LEVEL OF THE ANTERIOR NEUROPORE
*FOREGUT
*ORAL PLATE
*SUBCEPHALIC POCKET
B. LEVEL OF THE AMNIO-CARDIAC VESICLE
*SPLANCHNOPLEURE
C. LEVEL OF THE ANTERIOR INTESTINAL PORTAL
D. LEVEL OF THE MIDGUT
*CARDIAC PRIMORDIA= thickened splanchnic mesoderm
E. LEVEL OF THE SOMITE
F. LEVEL OF THE PRIMITIVE PIT
*AREA OPACA VASCULOSA
*AREA OPACA VITELLINA
V. WHOLE MOUNT OF A 33-HOUR CHICK EMBRYO
*PROSOCOEL
*ANTERIOR NEUROPORE
*NOTOCHORD
*SINUS RHOMBOIDALIS
*AUDITORY/OTIC VESICLE
*AORTIC ARCHES
*VITELLINE PLEXUS
*SINUS TERMINALIS
VI. SERIAL TRANSVERSE SECTION OF A 33-HOUR CHICK EMBRYO
A. LEVEL OF THE PROSENCEPHALON
*OPTIC VESICLES
*OPTOCOEL
*ECTODERM
*MESENCHYME
*BLOOD ISLANDS
*HEAD FOLD
*LATERAL BODY FOLD/ LATERAL LIMITING SULCUS
B. MESENCEPHALON
*FOREGUT
*ORAL PLATE
C. LEVEL OF THE HEART
*OTIC PLACODE
D. LEVEL OF THE MIDGUT
*ANTERIOR INTESTINAL PORTAL
*OMPHALOMESENTERIC VEINS
E. LEVEL OF THE SOMITE
*COELOM
brought to you by: BOGS!
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