allosteric enzymes -...
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Allosteric Enzymes
Allosteric enzyme kinetics
Determination of cooperativity coefficient
Inhibited Enzyme Kinetics
� Inhibitor� Inhibitor binds to enzymes and reduces their activity.
� Irreversible inhibitor� Heavy metals form a stable complex with enzymes.� Heavy metals form a stable complex with enzymes.
� Pd, Cd, Hg, and others
� May be reversed only by using chelating agents such as EDTA and citrate.
� Reversible inhibitor� Dissociate more easily from the enzyme
Reversible Enzyme Inhibition
� Competitive inhibition
� Noncompetitive inhibition
� Uncompetitive inhibition
� Substrate inhibition
Competitive Inhibition
L-B Plot
Noncompetitive Inhibition
L-B Plot
Uncompetitive Inhibition
L-B Plot
Substrate Inhibition
Substrate Inhibition
L-B Plot
L-B Plot
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