aproject report march month
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Table 4.3 Showing positive growth over Pseudomonas isolation media.
Positive growth of the samples Station Road Sample no.1 (New Cake House), Sample no.4 (Krishna Bakery)
Rama Mandi Sample no.1 (Flora Bakery), Sample no.2 (Gurunanak Bakery) and
Sample no. 4 (Ashwani Confectioner)
Jyoti Market Sample no. 1(Friends Bakery), Sample no.3 (global Bakery Shop) and
Sample no.4 (Gulshan Bakery)
Model town Sample no.1 (Prakash Bakery)
Nakodar Chowk Sample no.1 (Fancy Bakery) and Sample no.3 (DashmeshBakery)
Sample no.2 Station road Sample no.4 Station road
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Sample no.1 Rama mandi Sample no.4 Rama mandi Sample no.2 Rama mandi
Sample no.1 Jyoti Market Sample no.3 jyoti Market Sample no.4 Jyoti Market
Sample no.1 Model Town
Sample no.1Nakaudar Chawk Sample no.-3 Nakaudar Chawk
Fig4.1 Showing bacterial growth of Samples over the pseudomonas selective media collected from different areas.
Preparation of Pure Culture for Pseudomonas aeruginosa
For preparing pure culture single colony has been isolated and then suspended into the nutrient broth.
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Gram’s staining
Pseudomonas aeruginosa is a gram negative bacteria
Sample no.-2 Station Road Sample no.-4 Station Road
Sample no.-1 Rama Mandi Sample no.-2 Rama Mandi Sample no.-4 Rama Mandi
Sample no.-1Jyoti Market Sample no.-3 Jyoti Market Sample no.4 Jyoti Market
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Sample no.-1Model Town
Sample no.-1Nakaudar Chawk Sample no.-3 Nakaudar Chawk
Fig 4.2All figures showing gram’s negative results
Biochemical Tests
Biochemical tests has been done for cross checking the presence of this
bacteria
Gelatin Hydrolysis
It gives positive result on gelatin hydrolysis which had been visualised by
liquefaction of gelatine agar.
Fig 4.3Showing positive results of Gelatin Hydrolysis
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Citrate Utilization Test Colour changing from green to blue indicates that Pseudomonas
aeruginosa were present in the sample.
Fig 4.4Showing positive results of Citrate Utilization Test
Confirmatory test for E.coli
Growth on selective media
Out of different isolated bacteria some were found to be grown on the
EMB agar selective media
Table no. 4.4 showing positive growth over the EMB media
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Sample no.-3 Station Road Sample no.2 Station Road
Sample no.-2 Rama Mandi Sample no.-4 Rama Mandi
Sample no.3 Jyoti Market Sample no.1 Jyoti Market Sample no.2 Jyoti Market
Sample no.4 Jyoti Market
Sample no.1 Model Town Sample no.2 Model Town Sample no.4 Model Town
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Sample no.3 Model Town
Sample no.4 Nakaudar Chawk Sample no.3 Nakaudar Chawk
Fig 4.5 Showing bacterial growth of Samples over the EMB media collected from different areas.
Preparation of Pure Culture for E. coli
For preparing pure culture single colony has been isolated and then suspended into the nutrient broth.
Gram’s Staining
E. coli is a gram negative bacteria.
Fig. 4.6 showing gram negative bacteria by doing Gram’s staining.
After Gram’s staining 14 samples were found as negative bacteria
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Biochemical test
Biochemical tests has been done for cross checking the presence of this bacteria
Indole Test
Fig 4.7 Showing positive results of IndoleTest
MethylRed-VogesProskauer TEST
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Fig 4.8 Showing positive results of Methyl RedTest
Fig4.9 showing negative results of Voges Proskauer Test for E. coli
Citrate Utilization Test
Fig 4.10 showing negative results of Citrate Utilization Test for E.coli
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Comparison with another bakery product (Biscuits)
For observing which part of the pastry is more contaminated compared with another bakery
product biscuits. For this ,Parley G biscuits were chosen and the same protocol were followed
as done in the case of Pastry sample .Further, cream and bakery part of the pastry were
separately observed for bacterial growth.
Results
Fig 4.11 showing minimum growth over the NA agar plates from biscuits samples
Fig 4.12 showing no growth over the EMB agar plates from biscuits samples
Fig 4.13 showing no growth over the Pseudomonas isolation agar plates from biscuits samples
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For Pseudomonas aeruginosa
Name of Area Positive growth over
the media from cream part
Positive growth over the media from bakery part
Station Road Sample no.4(Krishna Bakery)
-------
Rama Mandi Sample no.2(Gurunanak Bakery)
------
Jyoti Market Sample no. 1(Friends Bakery)
Sample no.3(global Bakery Shop)
Model town --------- -------- Nakodar Chowk Sample no.1(Fancy Bakery) Sample
no.3(DashmeshBakery) Table no. 4.5 showing comparative results of growth over the Pseudomonas isolation media from creamy and bakery part of pastry
Fig4.14 showing positive growth over the Pseudomonas isolation agar plates, samples taken from the creamy part
Fig4.15 showing positive growth over the Pseudomonas isolation agar plates, samples taken from the Bakery part.
For E.coli
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Table 4.6 Showing comparative results of growth over the EMB media from creamy and bakery part of pastry
Fig4.16 showing positive growth over the EMB agar plates, samples taken from the creamy part.
Fig4.17 showing positive growth over the EMB agar plates, samples taken from the bakery part.
Result
On the basis of this experiment, it can be said that the creamy part of the pastry is more susceptible for bacterial.
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Name of Area Positive growth over the media from the creamy part
Positive growth over the media from the bakery part
Station Road Sample no.1(New Cake House)
-------------
Rama Mandi ---------- ----------- Jyoti Market ---------- Sample no.3(global Bakery
Shop) Model town --------- ------------ Nakodar Chowk Sample no.1(Fancy Bakery) Sample
no.3(DashmeshBakery)
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