chapter 5 - queen's universitypost.queensu.ca/~biol330/chapter 5-replication.pdf · chapter 5...
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Chapter 5
Replication
235-266
Replication
Semi conservative replication
Synthesis in a 5’ to 3’ direction
DNA polymerase
DNA polymerase
It must be perfect!!
WRONG!!
The replication fork
Understand why there is a leading strand and a lagging strand
Mistakes happen!
Chew back and do it again
Priming the replication reaction in the lagging strand
Synthesizing the lagging strand
Helicase
Forcing the double stranded DNA apart!
The replication fork
The “actual” replication fork
SEM of replication fork
•
What releases the rotational pressure on the DNA?
Topoisomerase
What happens to the histones?
Histones are evenly shared between daughter strands, and new histones fill in the gaps
What happens on the lagging strand at the end of the chromosome?
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