choosing the appropriate camera “resolving” the application

Choosing the Appropriate Camera

“Resolving” the Application

The Appropriate Camera

•If you know the application AND the product, you will win every time

•If you know either the application or the product, you may win half the time

•If don’t know either, you will lose

How do I Select the Correct Product?

•Guess

•Pick The Cheapest

•Pick the Most Expensive

•Call Scott Monroe

•“Resolve” the Application

Resolving the Application:

Provide the Appropriate Resolution for the Application

SpatialSpatial TemporalTemporal

IntensityIntensity

Appropriate “Resolution”

Spatial Resolution

SpatialSpatial

Spatial Resolution

Size of pixels

0.61 N.A.

Mag = 2 pixels.?

100 x

1.3 NA

Spatial Resolution

Number of pixels

Field of View !!Field of View !!

Temporal Resolution

TemporalTemporal

Temporal Resolution

•What is your “Real Time”•How much light do you have in a given time interval?

Considerations:

•Digitizer Readout Rate•CCD Architecture•Quantum Efficiency•Read Noise

Temporal Resolution

Region of InterestRegion of Interest Frame TransferFrame Transfer

Small Array SizeSmall Array Size

InterlineInterline

Temporal Resolution

e

e

ICCD

Intensity Resolution

IntensityIntensity

Intensity Resolution

•Dim and Bright sources within the same Image

•Measure small differences in Intensities

•Measure small Fluctuations on a large background

Dynamic Range

Intensity Resolution

electronselectrons

GrayGrayValuesValues

00

40954095

20,000 40,000 80,000

Signal Mapping to the ADC

Best of “Both Worlds”

SpatialSpatial TemporalTemporal

IntensityIntensity

High Spatial and Intensity Resolution

Best of “Both Worlds”

High Spatial and Intensity Resolution

Full Frame AcquisitionFull Frame Acquisition

Best of “Both Worlds”

SpatialSpatial TemporalTemporal

IntensityIntensity

High Temporal and Intensity Resolution

Best of “Both Worlds”

High Temporal and Intensity Resolution

Binning: On Chip Charge CombinationBinning: On Chip Charge Combination

Best of “Both Worlds”

High Temporal and Intensity Resolution

On-Chip Multiplication GainOn-Chip Multiplication Gain

Best of “Both Worlds”

SpatialSpatial TemporalTemporal

IntensityIntensity

High Spatial and Temporal Resolution

Best of “Both Worlds”

High Spatial and Temporal Resolution

( X , Y )( X , Y ) ( X , t )( X , t )

““Shoot and Shift” or “Kinetics Mode”Shoot and Shift” or “Kinetics Mode”

Best of “Both Worlds”

High Spatial and Temporal Resolution

( X , Y )( X , Y ) ( x, y , t )( x, y , t )

““Virtual Chip Mode”Virtual Chip Mode”

Biological Products

Microscopy Products

•Tier 1– CoolSNAPcf Color and Mono

•Tier 2– CoolSNAPES and CoolSNAPHQ

•Tier 3– SenSys, Quantix, Cascade

•Tier 4– MicroMAX Backthinned, I-PentaMAX

Price Points:

1x

4-6x

2x

3x

8x

Biological Products

Camera Type Array Size Pixel Size(m) Bit Depth Feature CoolSNAPcf Interline 1392x1030 4.65 12 Hyper HAD SenSys 1401E Full Frame 1317x1035 6.8 12 ITO CoolSNAPes Interline 1392x1040 6.45 12 Hyper HAD CoolSNAPHQ Interline 1392x1040 6.45 12 Super HAD, EXview MicroMax 512BFT Frame Transfer 512x512 13 16 Back Illuminated Quantix 57 Frame Transfer 535x512 13 12 Back Illuminated MicroMax 1024B Full Frame 1024x1024 13 16 Back Illuminated I-PentaMax ICCD, FT 512x512 15 12 Gen 3 Intensifier Cascade 512F Frame Transfer 512x512 16 16 On-Chip Gain Cascade 512B Frame Transfer 512x512 16 16 On-Chip Gain, BI

SpatialSpatial TemporalTemporal

IntensityIntensity

HQ/es/cf

Roper Scientific Cameras:CoolSNAP

1.3 Mega PixelsSmall Pixels

20MHzInterline Architecture

SpatialSpatial TemporalTemporal

IntensityIntensity

HQ/es/cf

Roper Scientific Cameras

SenSys

1.4 Mega PixelsSmall Pixels

Full Frame Architecture

SpatialSpatial TemporalTemporal

IntensityIntensity

HQ/es/cf

Roper Scientific Cameras

512BFT

SenSys

Frame Transfer Architecture

16-bit 100kHz, 1MHz

SpatialSpatial TemporalTemporal

IntensityIntensity

HQ/es/cf

Roper Scientific Cameras

512BFT

SenSysQT 57

Frame Transfer Architecture

12-bit, 1-3MHz

SpatialSpatial TemporalTemporal

IntensityIntensity

HQ/es/cf

Roper Scientific Cameras

512BFT

SenSys

1024B

QT 57

16-bit 100kHz, 1MHz

1.0 Mega Pixels

Large pixels

SpatialSpatial TemporalTemporal

IntensityIntensity

HQ/es/cf

Roper Scientific Cameras

512BFT

SenSys

1024B

QT 57 I-PMax

Gen 3 IntensifierFrame Transfer Architecture

SpatialSpatial TemporalTemporal

IntensityIntensity

HQ/es/cf

Roper Scientific Cameras

512BFT

SenSys

1024B

QT 57 I-PMax

On-Chip Multiplication GainFrame Transfer Architecture

512B

16 micron pixels

Where Does Your Application Fit?

?

SpatialSpatial TemporalTemporal

IntensityIntensity

HQ/es/cf

512BFT

SenSys

1024B

QT 57 I-PMax

512B

Where Does Your Application Fit?

SpatialSpatial TemporalTemporal

IntensityIntensity

Cortical Imaging ala Toga

HQ/es/cf

512BFT

SenSys

1024B

QT 57 I-PMax

512B

?

Where Does Your Application Fit?

SpatialSpatial TemporalTemporal

IntensityIntensity

Tirf Res/Tirf Fluor

HQ/es/cf

512BFT

SenSys

1024B

QT 57 I-PMax

512B

?

Where Does Your Application Fit?

SpatialSpatial TemporalTemporal

IntensityIntensity

Embyonic Chick

HQ/es/cf

512BFT

SenSys

1024B

QT 57 I-PMax

512B

?

Where Does Your Application Fit?

SpatialSpatial TemporalTemporal

IntensityIntensity

Single Molecule Fluor

HQ/es/cf

512BFT

SenSys

1024B

QT 57 I-PMax

512B

?

Where Does Your Application Fit?

SpatialSpatial TemporalTemporal

IntensityIntensity

Live Cell Fluor

HQ/es/cf

512BFT

SenSys

1024B

QT 57 I-PMax

512B

?