choosing the appropriate camera “resolving” the application
TRANSCRIPT
Choosing the Appropriate Camera
“Resolving” the Application
The Appropriate Camera
•If you know the application AND the product, you will win every time
•If you know either the application or the product, you may win half the time
•If don’t know either, you will lose
How do I Select the Correct Product?
•Guess
•Pick The Cheapest
•Pick the Most Expensive
•Call Scott Monroe
•“Resolve” the Application
Resolving the Application:
Provide the Appropriate Resolution for the Application
SpatialSpatial TemporalTemporal
IntensityIntensity
Appropriate “Resolution”
Spatial Resolution
SpatialSpatial
Spatial Resolution
Size of pixels
0.61 N.A.
Mag = 2 pixels.?
100 x
1.3 NA
Spatial Resolution
Number of pixels
Field of View !!Field of View !!
Temporal Resolution
TemporalTemporal
Temporal Resolution
•What is your “Real Time”•How much light do you have in a given time interval?
Considerations:
•Digitizer Readout Rate•CCD Architecture•Quantum Efficiency•Read Noise
Temporal Resolution
Region of InterestRegion of Interest Frame TransferFrame Transfer
Small Array SizeSmall Array Size
InterlineInterline
Temporal Resolution
e
e
ICCD
Intensity Resolution
IntensityIntensity
Intensity Resolution
•Dim and Bright sources within the same Image
•Measure small differences in Intensities
•Measure small Fluctuations on a large background
Dynamic Range
Intensity Resolution
electronselectrons
GrayGrayValuesValues
00
40954095
20,000 40,000 80,000
Signal Mapping to the ADC
Best of “Both Worlds”
SpatialSpatial TemporalTemporal
IntensityIntensity
High Spatial and Intensity Resolution
Best of “Both Worlds”
High Spatial and Intensity Resolution
Full Frame AcquisitionFull Frame Acquisition
Best of “Both Worlds”
SpatialSpatial TemporalTemporal
IntensityIntensity
High Temporal and Intensity Resolution
Best of “Both Worlds”
High Temporal and Intensity Resolution
Binning: On Chip Charge CombinationBinning: On Chip Charge Combination
Best of “Both Worlds”
High Temporal and Intensity Resolution
On-Chip Multiplication GainOn-Chip Multiplication Gain
Best of “Both Worlds”
SpatialSpatial TemporalTemporal
IntensityIntensity
High Spatial and Temporal Resolution
Best of “Both Worlds”
High Spatial and Temporal Resolution
( X , Y )( X , Y ) ( X , t )( X , t )
““Shoot and Shift” or “Kinetics Mode”Shoot and Shift” or “Kinetics Mode”
Best of “Both Worlds”
High Spatial and Temporal Resolution
( X , Y )( X , Y ) ( x, y , t )( x, y , t )
““Virtual Chip Mode”Virtual Chip Mode”
Biological Products
Microscopy Products
•Tier 1– CoolSNAPcf Color and Mono
•Tier 2– CoolSNAPES and CoolSNAPHQ
•Tier 3– SenSys, Quantix, Cascade
•Tier 4– MicroMAX Backthinned, I-PentaMAX
Price Points:
1x
4-6x
2x
3x
8x
Biological Products
Camera Type Array Size Pixel Size(m) Bit Depth Feature CoolSNAPcf Interline 1392x1030 4.65 12 Hyper HAD SenSys 1401E Full Frame 1317x1035 6.8 12 ITO CoolSNAPes Interline 1392x1040 6.45 12 Hyper HAD CoolSNAPHQ Interline 1392x1040 6.45 12 Super HAD, EXview MicroMax 512BFT Frame Transfer 512x512 13 16 Back Illuminated Quantix 57 Frame Transfer 535x512 13 12 Back Illuminated MicroMax 1024B Full Frame 1024x1024 13 16 Back Illuminated I-PentaMax ICCD, FT 512x512 15 12 Gen 3 Intensifier Cascade 512F Frame Transfer 512x512 16 16 On-Chip Gain Cascade 512B Frame Transfer 512x512 16 16 On-Chip Gain, BI
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras:CoolSNAP
1.3 Mega PixelsSmall Pixels
20MHzInterline Architecture
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
SenSys
1.4 Mega PixelsSmall Pixels
Full Frame Architecture
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSys
Frame Transfer Architecture
16-bit 100kHz, 1MHz
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSysQT 57
Frame Transfer Architecture
12-bit, 1-3MHz
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSys
1024B
QT 57
16-bit 100kHz, 1MHz
1.0 Mega Pixels
Large pixels
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSys
1024B
QT 57 I-PMax
Gen 3 IntensifierFrame Transfer Architecture
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSys
1024B
QT 57 I-PMax
On-Chip Multiplication GainFrame Transfer Architecture
512B
16 micron pixels
Where Does Your Application Fit?
?
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Cortical Imaging ala Toga
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Tirf Res/Tirf Fluor
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Embyonic Chick
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Single Molecule Fluor
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Live Cell Fluor
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?