cosmosil hplc columns - winlab · 2020. 4. 28. · cosmosil® hplc columns sample separation mode...
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COSMOSIL® HPLC Columns
Sample Separation Mode
Packing Material
USP Code
Stationary Phase
Special Features and Applications
Organic Compounds (low M.W.)
Reversed Phase
3C18-EB L1 Octadecyl Group Excellent for basic compounds
3 µm silica packing material
C18-MS-II L1 Octadecyl Group Multi-purpose C18column Monofunctional silylation
on ultra-pure silica gel for separation of the widest range of compounds
C18-AR-II L1 Features strong acid resistance and suitable for acidic compounds and peptides
C18-PAQ L1 Reversed phase column, compatible with 100% water based mobile phase.
Suitable for hydrophilic compounds
Cholester L101 Cholesteryl Group
Usable under the same condition as C18
Unique rigid cholesteryl structure improves separation
PBr - Pentabromo-benzyl group
Separate hydrohilic compounds in reversed-phase conditions
πNAP - Naphtylethyl Group
Stronger π-π interaction than Phenyl column
PYE - Pyrenylethyl Group
Most powerful π-π interaction
NPE - Nitrophenylethyl Group
Separation utilizing π-π interaction and dipole-dipole interaction
PBB-R - Pentabromo-benzyl Group
Separation utilizing dispersion force
PFP L43 Pentafluoro-phenyl Group
Separation by dipole-dipole interactions.
CN-MS L10 Cyanopropyl Group
Great reproducibility using isocratic elution mode
C22-AR-II - Docosyl Group
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Sample Separation Mode
Packing Material
USP Code
Stationary Phase
Special Features and Applications
C8-MS L7 Octyl Group Alkyl chain columns except C18 column C4-MS L26 Butyl Group
TMS-MS L13 Trimethyl Group
PE-MS L11 Phenylethyl Group
π-π interaction
Normal Phase
SL-II L3 - Suitable for preparative separation
Hydrophilic Interaction
HILIC L104 Triazole Retains highly polar compounds that would not be retained in C18column
Mono-saccharides Oligo-saccharides
Hydrophilic Interaction
Sugar-D - Secondary/ Tertiary Amine
Suitable for monosaccharides, oligosaccharides
NH2-MS L8 Aminopropyl Group
Primary amine bonded column
Proteins Reversed Phase
Protein-R L1 Octadecyl Group Provides improved separation, high recovery rate, and acid resistance
C18-AR-300 L1 Octadecyl Group Wide pore type reversed phase columns
C8-AR-300 L7 Octyl Group
C4-AR-300 L26 Butyl Group
Ph-AR-300 L11 Phenyl Group
Proteins Gel Permeation
Diol-120-II L20 Diol Group Silica-based gel filtration column
Sample MW (Protein) 5,000-100,000 Da
Diol-300-II Silica-based gel filtration column
Sample MW (Protein) 10,000-700,000 Da
Diol-1000-II
Silica-based gel filtration column
Sample MW (Water Soluble Polymer) 50,000 - 500,000 Da
Ion-exchange
Type Q Quaternary Ammonium Type
Anion-exchange Type - For Purification
Target Sample: Acidic Proteins / DNA
Type Q-N Anion-exchange Type For Ultra-fast Analysis,
Precise Analysis
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Sample Separation Mode
Packing Material
USP Code
Stationary Phase
Special Features and Applications
Target Sample: Acidic Proteins / DNA
Type S Sulfopropyl Type Cation-exchange Type For Purification Target Sample: Basic Proteins
Type S-N Cation-exchange Type - For Ultra-fast Analysis, Precise Analysis - Target Sample: Basic Proteins
Type M Quaternary Ammonium Type/Sulfopropyl Type
Amphoteric ion-exchange Type - For Purification - Target Sample: All Proteins
Type M-N Amphoteric ion-exchange Type - For Precise Analysis - Target Sample: All Proteins
Hydrophobic Interaction
HIC - - Suitable for separation of peptide, and there is only a little less in enzyme activity and the teriary structure of proteins
Fullerenes - Buckyprep - Pyrenylpropyl Group
Standard column for fullerene separation
Buckyprep-D
- Nitro-carbazoyl Group
Designed for separation of fullerene derivatives
Buckyprep-M
- Phenothiazinyl Group
Designed to separate metallofullerenes
PBB - Pentabromo-benzyl Group
Designed for preparative separation of C60, C70
PYE - Pyrenylethyl Group
Separation of Fullerenes and structural isomers
NPE - Nitrophenylethyl Group
Separation of derivatived fullerene
Carbon Nanotubes
Gel Permeation
CNT Hydrophilic Group
Separation of carbon nanotubes
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Selection Guide
Organic Compounds (Low M.W.) Octadecyl group bonded column (C18ODS) are recommended as first-choice columns for separations of organic compounds (Low M.W.). If there is not enough separation or no retention using COSMOSIL C18 columns, COSMOSIL series offer many kinds of special columns.
Please click here for the selection guide for C18 columns
Saccharides COSMOSIL Sugar-D is recommended for the separation of monosaccharides and
oligosaccharides as a first-choice column. For the separation of sugar derivatives, COSMOSIL C18-PAQ is suitable as well. Proteins Please select based on the separation mode. Fullerenes COSMOSIL Buckyprep is most suitable for the separation of fullerenes. COSMOSIL Specialty Columns Click here
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USP Specifications
The USP (U.S. Pharmacopeia) specifications are listed below with the recommended COSMOSIL HPLC columns.
USP Code
Description Recommended Columns
L1 Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 1.7 to 10 m in diameter, or a monolithic rod
COSMOSIL 3C18EB COSMOSIL C18-MS-II COSMOSIL C18-AR-II COSMOSIL C18-PAQ COSMOSIL C18-AR-300 COSMOSIL Protein-R
L3 Porous silica particles, 5 to 10 µm in diameter COSMOSIL SL-II
L7 Octylsilane chemically bonded to totally porous silica particles, 1.7 to 10 µm in diameter
COSMOSIL C8-MS COSMOSIL C8-AR-300
L8 An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10 µm in diameter, or a monolithic silica rod.
COSMOSIL NH2-MS
L10 Nitrile groups chemically bonded to porous silica particles, 3 to 10 µm in diameter
COSMOSIL CN-MS
L11 Phenyl groups chemically bonded to porous silica particles, 1.7 to 10 µm in diameter
COSMOSIL PE-MS COSMOSIL Ph-AR-300
L13 Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diamete
COSMOSIL TMS-MS
L20 Dihydroxypropane groups chemically bonded to porous silica particles, 5 to 10 µm in diameter
COSMOSIL Diol-120-II COSMOSIL Diol-300-II COSMOSIL Diol-1000-II
L26 Butyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter
COSMOSIL C4-MS COSMOSIL C4-AR-300
L43 Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameter
COSMOSIL PFP
L101 Cholesteryl groups chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod.
COSMOSIL Cholester COSMOCORE Cholester
L104 Triazole groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter
COSMOSIL HILIC
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