doctoral students session, turek iga, leukocyte sirtuin 1 mrna overexpression is associated with...

Leukocyte Sirtuin 1 mRNA overexpression is associated

with Gestational Diabetes Mellitus (GDM)

Iga A. TurekDepartment of Structural Biology

Medical University of LodzSupervisor: Prof. Lucyna A. Woźniak

Co-supervisor: Dr Marzena Wójcik

Presentation overview

1. Introduction

– Gestational Diabetes Mellitus (GDM)

– Sirtuin 1 (SIRT1)

2. Project aims

3. Methodology

4. Results

5. Conclusions

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Introduction

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GDM (Gestational Diabetes Mellitus) glucose intolerance with onset during

pregnancy. prevalence: up to 17%

SIRT1 (Sirtuin 1) NAD+-dependent histone deacetylase maintenance of glucose and lipid

homeostasis regulation of insulin secretion over 20 known substrates

Project aims:

to determine SIRT1 mRNA expression in leukocytes of GDM women at 24-33 weeks of pregnancy

to correlate changes in leukocyte SIRT1 expression with clinical characteristics of subjects

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Methodology

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RNA isolation

leukocytes separation

GDM (the HAPO criteria)n=135

control group (NGT)n=52

venous blood

leukocytes

RNA

RBC lysis buffer in hypotonic conditions

qRT-PCR

SIRT1

Primer sequence 5’→3’ cDNA amplicon size

Reverse: TGTGACAGAGAGATGGCTGG142

Forward: GCTCGCCTTGCTGTAGACTT

Results

SIRT1 expression leukocyte SIRT1 mRNA expression was 1.7-fold higher in GDM vs.

NGT pregnant women

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Correlations of SIRT1 expressionPositive correlations of SIRT1 expression with:A.glucose concentration at 2 h of OGTT in the whole study group (NGT + GDM)B.HDL-cholesterol in NGT group

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Results:

increased leukocyte SIRT1 mRNA expression in the GDM women

association of leukocyte SIRT1 overexpression with hyperglycemia in diabetic subjects

positive correlation between leukocyte SIRT1 expression and HDL-cholesterol in health pregnant women

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Conclusions:

association of GDM with a change in leukocyte SIRT1 expression at its gene level

leukocyte SIRT1 expression is related to hyperglycemic conditions

potential protective role of leukocyte SIRT1 against CVD in healthy pregnancy

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Thank you for your attention