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Clostridium, Bacteriods & AntibioticsClostridium, Bacteriods & AntibioticsClostridiumClostridium 33Station 1: Gram stain of Clostridium perfringens 4

Station 2: Spore stain of Clostridium perfringens 5

Station 3: Growth on blood agar 6

Station 4: Growth cooked meat medium 7

Station 5: Nagler reation 8

Station 6: Growth on litmus milk medium 9

BacteroidsBacteroids 1010Station 7: Gram stain 11

Tools used for growing the anerobes and sensitivity testingTools used for growing the anerobes and sensitivity testing 1212Station 8: Anaerobiosis 13

Station 9: Disc antibiotic susceptibility 14

Station 10: Disc antibiotic diffusion on agar 15

Station 11: Treatment & diseases 16

Station 12: Summary 17

= Perfringens

Station 1Station 1

Station 2Station 2

ββ Haemolitic activity Haemolitic activity

(double zone of heterolysis)(double zone of heterolysis)

No HaemolysisNo Haemolysis

““little colonies”little colonies”

Station 3Station 3

Digested

Black color

Station 4Station 4

Clostridium perfringens

Precipitations of lecithin that are Precipitations of lecithin that are broken down by lechithinase broken down by lechithinase prduced by C. Perfringensprduced by C. Perfringens

Station 5Station 5

Acid and gas are produced during the growth of Perfringens Acid and gas are produced during the growth of Perfringens with in the litmus milk producing:-with in the litmus milk producing:-

1- clot “because of acid”1- clot “because of acid”

2- pink color “ because of acid”2- pink color “ because of acid”

3- holes in the clot “because of gas passing through it” 3- holes in the clot “because of gas passing through it”

Station 6Station 6

Station 7Station 7

Station 8Station 8

This was explained This was explained previouslypreviously

Bacteria

Control

Station 9Station 9

Zone of inhibition

Station 10Station 10

Station 11Station 11

Clostridia perfringens

Infection:-1-Gas gangrene amputation of limbs2- Food poisoning “meat, stew & soup” vomiting and diarrhea

Treatment:-Cleaning the wound, putting the patient in hyperbaric oxygen chamber and give “Penicillin” in high doses

Clostridia tetani

Infection:-Tetanus, associated with cutting the umblical cord with unsertile blades.

Treatment:-Administrating antitoxins and giving “Penicillin” in high doses

No bacteremia (not invasive)

Bacterioids

Infection:-1- head, neck & respiratory tract infection2- lower abdomen and pelvis

Treatment:-“Metronidazole” and resistant to aminglycosides

Gram stainGram stain

TypeType

SporesSpores

Aerobe or Aerobe or anaerobe?anaerobe?

+ve -ve

bacilli bacilli yes no

anaerobes anaerobes

How to differentiate between clostridium perfringens & tetani (medium)?

Perfringens: Blood agar beta hemolysis, Neglar reaction & litmus milk reaction

Tetani: Cooked meet medium black

Station 12Station 12

يا يا دعواتكم دعواتكم

شبابشباب

A word can change the outcome of A word can change the outcome of

many things. So, if you either liked or many things. So, if you either liked or

didn’t like this project, why keep your didn’t like this project, why keep your

thoughts to yourself ?thoughts to yourself ?

Send us your comments and ideas Send us your comments and ideas

to to COORDINATOR1422@YAHOO.COM, It will

mean too much to us, “even if it was just “even if it was just

one word”one word”

THANXTHANX

M. F. ShaheenM. F. Shaheen 2003 2003