f1 press f1 for guideance. clostridium, bacteriods & antibiotics clostridium3 station 1: gram...
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MFSH MFSH 20032003
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Clostridium, Bacteriods & AntibioticsClostridium, Bacteriods & AntibioticsClostridiumClostridium 33Station 1: Gram stain of Clostridium perfringens 4
Station 2: Spore stain of Clostridium perfringens 5
Station 3: Growth on blood agar 6
Station 4: Growth cooked meat medium 7
Station 5: Nagler reation 8
Station 6: Growth on litmus milk medium 9
BacteroidsBacteroids 1010Station 7: Gram stain 11
Tools used for growing the anerobes and sensitivity testingTools used for growing the anerobes and sensitivity testing 1212Station 8: Anaerobiosis 13
Station 9: Disc antibiotic susceptibility 14
Station 10: Disc antibiotic diffusion on agar 15
Station 11: Treatment & diseases 16
Station 12: Summary 17
= Perfringens
Station 1Station 1
Station 2Station 2
ββ Haemolitic activity Haemolitic activity
(double zone of heterolysis)(double zone of heterolysis)
No HaemolysisNo Haemolysis
““little colonies”little colonies”
Station 3Station 3
Digested
Black color
Station 4Station 4
Clostridium perfringens
Precipitations of lecithin that are Precipitations of lecithin that are broken down by lechithinase broken down by lechithinase prduced by C. Perfringensprduced by C. Perfringens
Station 5Station 5
Acid and gas are produced during the growth of Perfringens Acid and gas are produced during the growth of Perfringens with in the litmus milk producing:-with in the litmus milk producing:-
1- clot “because of acid”1- clot “because of acid”
2- pink color “ because of acid”2- pink color “ because of acid”
3- holes in the clot “because of gas passing through it” 3- holes in the clot “because of gas passing through it”
Station 6Station 6
Station 7Station 7
Station 8Station 8
This was explained This was explained previouslypreviously
Bacteria
Control
Station 9Station 9
Zone of inhibition
Station 10Station 10
Station 11Station 11
Clostridia perfringens
Infection:-1-Gas gangrene amputation of limbs2- Food poisoning “meat, stew & soup” vomiting and diarrhea
Treatment:-Cleaning the wound, putting the patient in hyperbaric oxygen chamber and give “Penicillin” in high doses
Clostridia tetani
Infection:-Tetanus, associated with cutting the umblical cord with unsertile blades.
Treatment:-Administrating antitoxins and giving “Penicillin” in high doses
No bacteremia (not invasive)
Bacterioids
Infection:-1- head, neck & respiratory tract infection2- lower abdomen and pelvis
Treatment:-“Metronidazole” and resistant to aminglycosides
Gram stainGram stain
TypeType
SporesSpores
Aerobe or Aerobe or anaerobe?anaerobe?
+ve -ve
bacilli bacilli yes no
anaerobes anaerobes
How to differentiate between clostridium perfringens & tetani (medium)?
Perfringens: Blood agar beta hemolysis, Neglar reaction & litmus milk reaction
Tetani: Cooked meet medium black
Station 12Station 12
يا يا دعواتكم دعواتكم
شبابشباب
A word can change the outcome of A word can change the outcome of
many things. So, if you either liked or many things. So, if you either liked or
didn’t like this project, why keep your didn’t like this project, why keep your
thoughts to yourself ?thoughts to yourself ?
Send us your comments and ideas Send us your comments and ideas
to to [email protected], It will
mean too much to us, “even if it was just “even if it was just
one word”one word”
THANXTHANX
M. F. ShaheenM. F. Shaheen 2003 2003