fss-i 3 dna expert system suite. dna profiling sample e.g. hair, skin

FSS-i3

DNA expert system suite

DNA Profiling

• Sample e.g. hair, skin

DNA Profiling

• Sample e.g. hair, skin

• Process sample to obtain raw data

DNA Profiling

• Sample e.g. hair, skin

• Process sample to obtain raw data

• Produce a profile of the DNA

DNA Profiling

• Sample e.g. hair, skin

• Process sample to obtain raw data

• Produce a profile of the DNA

• Store profile in NDIS (National DNA Index System) or match to existing profile

Expert System profiling motivation

• Sample & data processing already heavily automated

Expert System profiling motivation

• Sample & data processing already heavily automated

• Sample data analysis; DNA profiling is the bottle neck.

Expert System profiling motivation

• Sample & data processing already heavily automated

• Sample data analysis; DNA profiling is the bottle neck.

• Two independent data reviews required to upload a profile to NDIS

Expert System profiling motivation

• Sample & data processing already heavily automated

• Sample data analysis; DNA profiling is the bottle neck.

• Two independent data reviews required to upload a profile to NDIS

• Save enormous amounts of review time

FSS-i3 Expert System

• FSS: Forensic Science Service of the UK

FSS-i3 Expert System

• FSS: Forensic Science Service of the UK• Developed a software suit called FSS-i3

– 6 years of R&D and validation

FSS-i3 Expert System

• FSS: Forensic Science Service of the UK• Developed a software suit called FSS-i3

– 6 years of R&D and validation

• Designed by forensic scientists for forensic scientists

FSS-i3 Expert System

• FSS: Forensic Science Service of the UK• Developed a software suit called FSS-i3

– 6 years of R&D and validation

• Designed by forensic scientists for forensic scientists

• Made available to forensic community– USA, Canada, Europe, Australia, New Zealand

FSS-i3 Expert System

• FSS: Forensic Science Service of the UK• Developed a software suit called FSS-i3

– 6 years of R&D and validation

• Designed by forensic scientists for forensic scientists

• Made available to forensic community– USA, Canada, Europe, Australia, New Zealand

• Full Audit system

i-STRess

• The core DNA interpretation tool

i-STRess

• The core DNA interpretation tool

• Produces single source DNA profiles using configurable rules & filters

i-STRess

• The core DNA interpretation tool

• Produces single source DNA profiles using configurable rules & filters

• Detect multi-person DNA mixtures within a profile & export to i-STReam…

i-STReam

• Unique functionality to resolve two person DNA mixtures

i-STReam

• Unique functionality to resolve two person DNA mixtures

• Allows previously unusable DNA profiles to be uploaded to DNA databases

i-ntegrity

• DNA samples/data can become contaminated: accidental DNA mixture

i-ntegrity

• DNA samples/data can become contaminated: accidental DNA mixture

• System flags possible profile contaminations for further review

i-ntegrity

• DNA samples/data can become contaminated: accidental DNA mixture

• System flags possible profile contaminations for further review

• Saves time: no more manual reviews of profiles

FSS-i3 Validation

• FSS-i3 developed from internal FSS expert systems– 6 years of testing

FSS-i3 Validation

• FSS-i3 developed from internal FSS expert systems– 6 years of testing

• 1 million+ profiles uploaded to UK National DNA DB

FSS-i3 Validation

• FSS-i3 developed from internal FSS expert systems– 6 years of testing

• 1 million+ profiles uploaded to UK National DNA DB

• FSS-i3 validation project:– 70 000 previously analyzed samples– Minor discrepancies in 0.03% of samples & each of these

samples were flagged by the system for further review

Technology used in FSS-i3

• Strategies for implementing rules: unknown

Technology used in FSS-i3

• Strategies for implementing rules: unknown

• User optimization: 20+ rules settings

Technology used in FSS-i3

• Strategies for implementing rules: unknown

• User optimization: 20+ rules settings

• Designed to run on stand-alone Pentium 3 PC’s

Design & Development of FSS-i3

• Development time: six years

Design & Development of FSS-i3

• Development time: six years

• “The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1)

Design & Development of FSS-i3

• Development time: six years

• “The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1)

• “i-STReam is based on scientific theory developed by Dr Peter Gill and Dr Tim Clayton” (1)

Conclusions: FSS-i3

• Consistent profiling of samples

Conclusions: FSS-i3

• Consistent profiling of samples

• Ability to interpret mixed DNA profiles

Conclusions: FSS-i3

• Consistent profiling of samples

• Ability to interpret mixed DNA profiles

• Check for contamination

Conclusions: FSS-i3

• Consistent profiling of samples

• Ability to interpret mixed DNA profiles

• Check for contamination

• Significant reduction in time spent on the above, due to automation

References

• (1) http://www.forensic.gov.uk/forensic_t/i3/index.htm

• (2) http://www.promega.com/profiles/802/profilesindna_802_08.pdf

• (3) http://forensics.marshall.edu/NEST/Nest%20PDFs/Documents/NIJEvalExpertSys.pdf

• (4) http://www.promega.com/profiles/1001/profilesindna_1001_11.pdf

• (5) http://www.kathyreichs.com/dnaprofiling.htm

• (6) http://www.biotechnologyonline.gov.au/human/dnaprofile.cfm