genomics: reading genome sequences assembly of the sequence annotation of the sequence
Post on 04-Jan-2016
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Genomics:
READING genome sequences
ASSEMBLY of the sequence
ANNOTATION of the sequence
carry out dideoxy sequencing
connect seqs. to make whole chromosomes
find the genes!
“FIRST” generation sequencing
M.L. Metzker 2010. Nature Genetics Reviews 11, 31. First, 5 slides, in order of Figs.
Ion torrentIon torrent
NanoporeNanopore
5 Selected Technologies. 2nd, 3rd, and 4th generation sequencing.
Using Metzker, M.L. 2010, and T.P. Niedringhaus 2011Anal. Chem. 83, 4327.
• Solexa - - ->Illumina
Allows both directions: “pair-end”
Life: Roche/454; Polonator
• Soon to be bought by Thermo Fischer
NONE USED IN ROCHE/LIFE 454 PYROSEQUENCING
Ion Torrent (BIU)
• http://www.iontorrent.com/1-trillion-front-end/
Pacific Biosciences
New Generation:TemplateWith hairpinsOn each end
Nanopore DNA sequencing
http://lifetech-it.hosted.jivesoftware.com/videos/1016
• ??? work
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