grds international conference on health and life (4)

November 16, 2014 1Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D

International Conference on Advances in Healthcare & Life Sciences

(Singapore) 8 – 9 November 2014

INFLUENCE OF Weisella paramesenteroides ISOLATE “DADIH”TO THE BOWEL FREQUENCE, SECRETORY

IMMUNOGLOBULIN A LEVEL AND HEIGHT OF ILEUM VILLI OF THE MICE EPEC INDUCED DIARRHEA

November 16, 2014 2Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D

ANDALAS UNIVERSITYPADANG, WEST SUMATERAINDONESIA

Laboratory of Biotechnology / Technology of Product Husbandry, Faculty of Animal Science, Andalas University, Padang, West Sumatera,

IndonesiaEmail : purwati17@yahoo.co .id

INTRODUCTION

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D3

Benefit of Research

researchers interested in studying the effect of these bacteria on diarrhea particularly levels of SigA and ileum villi.

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D10

MATERIAL AND

METODERESEARCH

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D11

• Experimental animals were randomized into two factors:

• The first factor (A) is the dose of probiotic administration, consist of a negative control: only given standard feed and water (A1), positive control: EPEC administered at a dose of 108 cfu/g (A2), W. paramesenteroides dose of 2 x 108cfu/g (A3), W. paramesenteroides dose of 2 x 109cfu/g (A4), W. paramesenteroides with a dose of 2 x 1010cfu/g.

• The second factor (B) is the duration of observation (hour), which consist of 0 hour (B1), 12 hours (B2), 24 hours (B3), 36 hours (B4)

Observed variables :

1. Examination of bowel frequency, 2. levels of SIgA and 3. height of ileum villi

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D14

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D15

RESULTS AND DISCUSSION RESULTS AND DISCUSSION

Tabel 1. Mean bowel frequency(times) of mice based on doses and duration of observation

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D16

A Factor(doses)

B Factor (duration of observation)

Total Mean SDB1= 0hour

B2=12 hours

B3 = 24 Hours

B4 = 36hours

A1=K (-) 4,00g 5,00g 4,50g 5,00g 18,50 4,63 1.52 A2=K (+)

5,00g 50,50a 41,00b 37,00c 133,50 33,384.09

A3=2x108

5,50g 48,50a 16,50e 11,00f 81,50 20,38

3.19

A4=2x109

5,00g 30,50d 20,00e 16,00e 71,50 17,882.99

A5=2x1010

5,50g 28,50d 12,50f 11,00f 57,50 14,382.68

Total 25,00 163,00 94,50 80,00 362,50 90,63Mean 5,00 32,60 18,90 16,00 72,50 18,13

Note : different small letter superscripted in different columns and rows has highly significant difference (P<0.01).

Table 2. MeanSIgA (µg/ml) in feces based on doses and duration of observation

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D17

Factor A(dose)

Factor B (duration of observation)

Total Mean SDB1 = 0 hours

B2 = 12 hours

B3 = 24 hours

B4 = 36 hours

A1 = K(-) 1.82e 1.80e 1.82e 1.81e 7.26 1.82 0.95A2 = K(+) 1.83e 1.57h 1.60g 1.66f 6.66 1.66 0.91A3 = 2x108 1.84e 1.53h 2.27b 2.63a 8.59 2.15 1.04A4 = 2x109 1.83e 1.59h 2.13c 2.26b 7.80 1.95 0.99A5 = 2x1010

1.82e 1.56h 1.96d 2.17c 7.52 1.880.97

Total 9.14 8.05 10.24 10.40 37.83 9.46

Mean 1.83 1.61 2.05 2.08 7.57 1.89

Note : different small letter superscripted in different columns and rows has highly significant difference (P<0.01).

Table 4. Mean of height of ileum villi (µm) based on doses and duration of observation

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D18

Factor A(Dose)

Factor B (duration of observation)

Total Mean SDB1 = 0 hours

B2 = 12 hours B3 = 24 hours B4 = 36 hours

A1 = K(-) 111.21g 111.01g 115.49g 116.66g 454.37 113.59 7.54

A2 = K(+) 111.86g 54.33k 91.38h 116.73g 374.30 93.58 6.84

A3 = 2x108 111.17g 66.72j 156.42d 187.26a 477.10 119.28 8.07

A4 = 2x109 112.32g 72.68i 129.19f 164.06c 535.72 133.93 7.73

A5 = 2x1010 114.99g 52.08k 150.54e 174.80b 482.41 120.60 7.85

Total 564.55 356.82 643.02 759.51 2323.90 580.98

Mean 112.91 71.36 128.60 151.90 464.78 116.20

Note : different small letter superscripted at different columns and rows showed highly different significance (P<0.01).

Conclusion

Giving W.paramesenteroides dosing 2x108cfu/g after 24 hours significantly decreased the frequency of bowel, increased levels of SIgA and improving ileum villi in mice EPEC-induced diarrhea.

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D19

Acknowledgements

This research was supported by Laboratory of Biotechnology / Technology of Product Husbandry, Faculty of Animal Science, Andalas University, Padang. Indonesia and Hibah Pascasarjana Nomor : 004/UN.16/PL/MT-HB-PC/2012 Date 24 January 2012 Prof. Drh. Hj. Endang Purwati, MS, Ph.D.

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

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THANK YOU

November 16, 2014Prof. Drh. Hj. ENDANG PURWATI R N, MS.,

Ph.D24