lcd-array kit meat 5.0 - specificity - chipron gmbh · donkey: acd-005-025 goat: acd-006-025 camel:...

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

1 | P a g e

CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ info@chipron.com

LCD-Array Kit MEAT 5.0 - Specificity

Control hybridisations with 250ng DNA/PCR.

Total DNA was extracted from fresh muscle tissue (200 mg) of each species using the

NucleoSpin Kit –FOOD, Macherey & Nagel, Germany, according to kit instructions.

DNA extracts were treated with RNase, calibrated to 100 ng/µl and species identity was

confirmed by partial sequencing of coxI or cytB genes.

PCR and hybridisation was performed according to the instructions of the LCD-Array Kit MEAT

5.0 Manual using 250ng DNA/PCR and the HotStar Taq Plus Mastermix, Qiagen, Germany.

No cross reactivity - apart from faint signals for Red deer with Reindeer DNA and vice versa -

could be detected (pink arrows).

Control DNAs are available at Chipron GmbH. Order codes: ACD-xxx-025 (see below)

Beef: ACD-001-025 Bison: ACD-002-025 Sheep: ACD-003-025 Horse: ACD-004-025

[Bos taurus ] [Bos bison ] [Ovis aries ] [Equus caballus ]

Donkey: ACD-005-025 Goat: ACD-006-025 Camel: ACD-007-025 Buffalo: ACD-008-025

[Equus asinus ] [Capra hircus ] [Camelus dromedarius ] [Bubalus bubalis ]

Pork: ACD-009-025 Kangaroo: ACD-010-025 Hare: ACD-011-025 Rabbit: ACD-012-025

[Sus scrofa ] [Macropus rufus ] [Lepus europaeus ] [Oryctolagus cunniculus]

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

2 | P a g e

CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ info@chipron.com

Reindeer: ACD-013-025 Roe deer: ACD-014-025 Red deer: ACD-015-025 Fallow deer: ACD-016-025

[Rangifer tarandus ] [Capreolus capreolus ] [Cervus elaphus ] [Dama dama ]

Springbok: ACD-017-025 Dog: ACD-018-025 Cat: ACD-019-025 Chicken: ACD-020-025

[Antidorcas marsupialis] [Canis lupus fam.] [Felis cattus] [Gallus gallus ]

Turkey: ACD-021-025 Goose: ACD-022-025 Ostrich: ACD-023-025 Mallard: ACD-024-025

[Meleagris gallopavo ] [Anser anser ] [Struthio camelus ] [Anas platyrhyncos ]

Muscovy Duck:ACD-025-025 Pheasant: ACD-026-025 NTC

[Cairina moschata ] [Phasianus colchicus ] PCR grade water