microbial metabolism enzymes sp10

Post on 09-Apr-2018

234 Views

Category:

Documents

0 Downloads

Preview:

Click to see full reader

TRANSCRIPT

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 1/17

M I C R O B I A L M E T A B O L ISME N Z Y M E S

C H A PT E R 5

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 2/17

Metabolism: sum of all chemical reactionswithin an organism.

Metabolism:

1. Anabolism:

2. Catabolism:

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 3/17

Metabolic Reactions

A metabolic pathway is a sequence of 

enzymatically catalyzed chemical reactions

in a cell which are determined by enzymes.

1. Exergonic

2. Endergonic

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 4/17

Enzymes:

Biological catalysts

Protein in nature

Reaction runs 8-10 billion times faster

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 5/17

Figure 5.2

Energy Requirements of a Chemical Reaction

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 6/17

Enzyme Structure

Holoenzyme

1.

Apoenzyme

2. Cofactor

1) Coe

nzymes

: N A D+,

FA D,

CoA2) Metal ions

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 7/17

Figure 5.3

Components of a Holoenzyme

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 8/17

Mechanism of Enzymatic Action

Active site of an enzyme

Enzyme-substrate complex

Specificity of active site

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 9/17

Figure 5.4a

The Mechanism of Enzymatic

Action

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 10/17

Relationship betweenEnzyme Structure and Function

10, 20

, 30 and 40structure of an enzyme

Change of enzyme shape

Change of active site----denaturation

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 11/17

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 12/17

Factors affecting enzymatic activity

Temperature

pH

Substrate concentration

Inhibitors

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 13/17

Figure 5.5a

Effect of Temperature on Enzyme Activity

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 14/17

Inhibitors

Competitive inhibition

Noncompetitive inhibition

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 15/17

Figure 5.7a b

Enzyme Inhibitors: Competitive Inhibition

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 16/17

Enzyme Inhibitors: Competitive Inhibition

8/8/2019 Microbial Metabolism Enzymes Sp10

http://slidepdf.com/reader/full/microbial-metabolism-enzymes-sp10 17/17

Figure 5.7a, c

Enzyme Inhibitors: Noncompetitive Inhibition

top related