microbiology charts
Post on 14-Apr-2018
222 Views
Preview:
TRANSCRIPT
-
7/30/2019 Microbiology Charts
1/17
1. Sheep Blood agar
2. Bacteroides Bile-esculin agar
3. Bile esculin agar
4. Brucella base agar
5. Blood with kanamycin and vancomycin
6. Phenylethyl alcohol agar (aerobic and anaerobic)
7. Bordet-gengou Blood agar
8. Buffered charcoal-yeast extract agar
9. Burkholderia cepacia selective agar
10. Campylobacter blood agar
11. Cefsulodin-Irgansan-Novobiovin agar
12. Chococlate agar
13. Chromagars
14. Simmons Citrate agar
15. Columbia agar with antibiotics
16. CTA sugars
17. Egg Yolk Agar
18. Eosin Methylene Blue Agar
19. Hektoen Enteric Agar
20. Lowenstein-Jensen medium21. Macconkey agar
22. Macconkey sorbitol agar
23. Mannitol salt agar
24. Modified Thayer Martin agar
25. Mueller Hinton agar
26. Mueller hinton agar with 4% NaCl and 6 ug Oxacillin
27. New York City Medium
28. Pseudomonas cepacia agar
-
7/30/2019 Microbiology Charts
2/17
29. Regan-Lowe medium
30. Salmonella-Shigella agar
31. Thiogylcolate broth
32. Thiosulfate citrate bile salts sucrose agar
33. Tinsdale agar
34. Triple Sugar Iron (TSI)
35. Kligers Iron Agar (KIA)
36. Trypticase Soy Agar
37. Urea Broth
38. Urea Agar
39. Xylose-Lysine-Desoxycholate Agar
40. Vaginalis Agar
-
7/30/2019 Microbiology Charts
3/17
Type
Enriched, Nonselective, and Differential (Detection of hemolysis)
Selective and Differential
Selective and Differential
Nonselective nutrient medium
Enriched and Selective
Selective
Enriched. May be made selective by adding antibiotics
Nonselective and Enriched
Selective and Differential
Selective
Selective and Differential
Enriched and Nonselective
Nonselective and Differential
Differential
Nonselective
Differential
Nonselective and Differential
Selective and Differential
Selective and Differential
NonselectiveSelective and Differential
Selective
Selective and Differential
Selective
Nonselective
Nonselective
Selective
Selective
-
7/30/2019 Microbiology Charts
4/17
Selective
Selective and Differential
Nonselective
Selective and Differential
Selective and Differential
Differential
Differential
Nonselective
Selective
Differential
Selective and Differential
Nonselective and Differential
-
7/30/2019 Microbiology Charts
5/17
Organisms That Will Grow
Fastidious/Nonfastidious organisms. Ex: Staph, Strep, GPR, Most Neisseria species, etc.
Isolation and Presumptive ID ofBacteroides fragilis group
Isolation of Enterococcus species
Cultivaton of many organisms
Isolation of obligately anaerobic organisms
Isolation of gram (+) organisms. Inhibition of gram (-) organisms
Isolation ofBordetella pertussis and B. parapertussis
Isolation ofLegionella. Will also support the growth ofBordetella, Brucella, and Francisella tularensis
Isolation of B. cepacia
Isolation of Campylobacter jejuni subspecies jejuni and C. coli
Isolation of Yersinia enterocolitica, which ferments mannitol and forms bullseye colonies. Also used to isolate Aeromonas from stool speci
Cultivation of fastidious organisms, including Haemophilus, Neisseria gonorrhoeae, and Brucella
Isolation of MRSA
Gram (-) bacteria ability to utilize citrate
Cultivation of many organisms
Determines if organisms can ferment various carbs: maltose, lactose, sucrose. Differentiates Neisseria and Moraxella species
Identifiation of certain anaerobic isolates
Isolation of nonfastidious GNR. Inhibition of gram (+) organisms. E. coli (Strong lactose fermenter), Shigella and Salmonella (Non-lactose f
Isolation ofSalmonella and Shigella. Inhibition of gram (+) organisms and some gram (-).
Cultivation of MycobacteriaInhibition of Gram (+) organisms. Isolation of many nonfastidious GNR, Ex. Enterics.
Isolation of E. coli H0157:H7
Isolation of S. aureus, which uses mannitol and turns medium yellow. Inhibition of most gram (-) and many gram (+) organisms.
Isolation of Neisseria gonorrhoeae
Antimicrobial Susceptibility Tests
Isolation of Neisseria gonorrhoeae
Isolation of Neisseria species
Isolation ofBurkholderia cepacia (Previously known as Pseudomonas cepacia)
-
7/30/2019 Microbiology Charts
6/17
Isolation of Bordetella pertussis and B. parapertussis
Isolation of Salmonella and Shigella. Inhibition of gram (+) organisms and some gram (-).
Supports the growth of aerobes, anaerobes, and facultatives
Isolation of Vibrio. Inhibition of many gram (+) and gram (-) organisms.
Isolation of Corynebacterium diphtheriae
Contains lactoe, sucrose, and glucose. Differentiates enteric organisms.
Differentiates gram (-) enteric bacilli.
Cultivation of fastidious and nonfastidious organisms. May be used as base for blood agar
Tests for the presence of the enzyme urease
Differentiates members of the Enterobacteriaceae group
Isolation of Salmonella and Shigella. Inhibition of gram (+) organisms and some gram (-).
Isolation of Gardnerella vaginalis, which is B-hemolytic on human blood.
-
7/30/2019 Microbiology Charts
7/17
Special Comments
Nutritional base
Inhibitors: Gentamicin and Bile
Bile salts and Esculin
Nutrients (Blood may be added)
Inhibits gram (+) organisms
Inhibitor: Phenylethyl alcohol
Glycerol, potato infusion, and blood. Nutrients. Also inactivate toxic substances.
Charcoal: Removes toxic substances. Nutrients: Yeast etract, cysteine, iron salts, and alpha-ketoglutarate
Isolated from respiratory secretions
Nutrient base, inhibitors: antimicrobial agents, blood may be added
Inhibitors: Cefsulodin, irgasan, novobiocin, bile salts, crystal violet. Carb: Mannitol
Nonfastidious organisms will also grow. Nutritional base, heated RBCs or other supplements
None
None
Nutrient, blood/antibiotics may be added
None
None
Inhibitors and pH indicators. Contains carbohydrate: lactose.
Inhibitor: Bile salts. pH indicators: Acid fuchsin and bromthymol blue. Carbs: Lactose, sucrose, salicin. H2S system.
Eggs, Malachite greenInhibitors: Bile salts and crystal violet. pH indicator: Neutral red. Carb: Lactose.
None
Inhibitor: 7.5% salt. pH indicator: Phenol red. Mannitol
None
Supplements may be added: Salt, cations (broth), sheep or lysed horse blood
None
None
Contains antimicrobial agents
-
7/30/2019 Microbiology Charts
8/17
None
Inhibitors: Bile salts, citrate, and brilliant green. pH indicator: Neutral red. Carb: Lactose. H2S system.
Reducing agents: Thioglycollate, cystine, sodium sulfite. Agar: Reduces O2 diffusion.
Inhibitors: Citrate, bile, and high pH. pH indicator: Bromthymol blue. Carb: Sucrose. H2S system.
Tellurite: inhibitors many organisms
None
None
Contains: Digested soybeans and casein
None
None
Inhibitor: Bile salts. pH indicator: Phenol red. Carbs: Lactose, sucrose, xylose. Lysine. H2S system
Contains: Nutrients, human blood
-
7/30/2019 Microbiology Charts
9/17
Bacitracin susceptibility
CAMP test
Hippurate Hydrolysis
PYR hydrolysis
Optichin susceptibility
Bile esculin hydrolysis
Salt-Tolerance Test
X and V Factor Requirement
Oxidase
Spot Indol
Catalase
Coagulase
Hydrogen sulfide production
Urease
Motility
Vibriotstatic compound, O/129 disc test
ONPG
Deoxycholate
Cefinase
-
7/30/2019 Microbiology Charts
10/17
Purpose/Principle
Some organisms are suseptible to 0.04 units of bacitracin. Inoculate agar for confluent growth; add dis
GBS make CAMP factor. S aureus makes B-lysin. Hemolysis enhanced when CAMP factor meets B-lysin.
Hippurate ---(Hippuricase)---> Sodium benzoate + glycine. Detect either benzoate or glycine. Benzoate
Chemical reaction - detects B-naphthylamine
Inoculate agar plate for confluent growth with isolate. Place disk onto inoculate area. Incubate plate;
40% bile inhibits many organisms. Esculin ---> Esculetin + Glucose. Esculetin + Ferric ions --> Black
6.5% salt inhibits many organisms. Inoculate broth with organism
Test organism inoculated onto trypticase soy agar or Mueller-Hinton agar, which lack X and V factors.
Chemical Reaction - Detects indophenol
Rub organism onto reagent-impregnated filter paper.
Place organism on slide and add drop of H2O2. Detect bubbles
Prepare saline suspension, mix in drop of plasma
H2S + Iron or lead ----> Black
Medium becomes alkaline as ammonia is produced
Stab organism into semisolid agar deep
Inoculate agar for confluent growth, add disks, incubate plate and measure zone
Chemical reaction - Yellow color change in positive reaction
Added to agar in order, visible pitting in the agar -- positive reaction
Beta-lactamase detection
-
7/30/2019 Microbiology Charts
11/17
Pos. Rxn
Susceptible: Any zone
Enhanced hemolysis
Benzoate: Precipitate, Glycine: Blue
Red
Susceptible: Zone > Cutoff
Black
Turbidity or acid pH
Growth around disk
Dark purple or Blue-black
Blue-green
Bubbles
Cell clumping
Black
Red/pink
Diffuse haze of growth throughout
Any zone
Yellow
Pitting in agar
Yellow to Red color change
-
7/30/2019 Microbiology Charts
12/17
Pos. Organism
Micrococcus, GAS
GBS
GBS, Listeria, C. jejuni
GAS, Enterococcus, Other gram (+) bacteria
S. pneumoniae
Enterococcus, GDS, Listeria
Enterococci
X only: H. ducreyi, V only: H. parainflu and H parahaemo., X and V: H. influ and H. haemo.
Neisseria, Moraxella, Pseudomonas, B. cepacia, Vibrio, Aeromonas, and others
E coli, P vulgaris, Morganella, Vibrio, Aeronomas, Plesiomonas, and others
Staph, Micrococcus, Corynebacterium, Many Bacillus, and others
S aureus
Edwardsiella, Salmonella, Proteus
K pneumoniae, Proteus, Morganella, Most Brucella, H pylori, and others
Vibrio and Campylobacter (Darting), Bacillus, Many enterics, Aeromonas, and others
Susceptible: Plesiomonas, Most V. cholerae
E coli, Citrobacter, K pneumoniae, Enterobacter, S marcescens
Enterics
N gonorrhoeae, H influenzae, M catarrhalis, Enterococcus, Staph, Anaerobic bacteria
-
7/30/2019 Microbiology Charts
13/17
Neg. Rxn
Resistant: No zone
No enhanced hemolysis
Benzoate: No precipitate, Glycine: No color
No color change
Resistant: No zone
No color
No turbidity or pH change
No growth around disk
No color change
No color
No bubbles
No clumping
No color
Yellow
Growth only near stab line
No zone
Colorless
No pitting in agar
No color change
-
7/30/2019 Microbiology Charts
14/17
Neg. Organism
Staph, Stomatococcus, GBS, Other B-strep
Groups A, C, D, F, and G Strep, Enterococci
Groups A, C, F, and G Strep, Enterococci, Helicobacter
Other Strep
Other Strep
Other Strep
GDS not enterococci, Other Strep
All other bacteria
Enterics, S. maltophilia, Acinetobacter, Gardnerella
Salmonella, K pneuoniae, Enterobacter, and others
Clostridium, Fusobacterium, and others
S saprophyticus, S epidermidis, Many other Staph species, Micrococcus
E coli, Shigella, Klebsiella, Enterobacter, Serratia
E coli, Shigella, Salmonella, S marcescens, P multocida
Corynebacterium, Lactobacillus, Shigella, Klebsiella, Brucella, and others
Resistant: Aeromonas
Salmonella, Proteus, Providencia, Morganella
Gram positive bacteria
Non-B-lactamase bacteria
-
7/30/2019 Microbiology Charts
15/17
Urinary tract
Genitals and STDs
Upper Respiratory Tract
Lower Respiratory Tract
Ocular
Skin and Soft Tissue (what about bone?)
Gastrointestinal + Agents of possible food poisoning
Central Nervous System
Circulatory (blood)
-
7/30/2019 Microbiology Charts
16/17
Pathogens
E coli, Other Enterobacteriaceae, S saprophyticus, P aeruginosa
N gonorrhoeae, C trachomatis, T pallidum, H ducreyi
S pyogenes, H influenzae
S pneumoniae, H influenzae, S aureus, M tuberculosis
S aureus, N gonorrhoeae, C trachomatis
B burgdorferi, R ricketsii
V cholerae, Salmonella, C jejuni, Shigella, C difficile, S aureus
C botulinum, C tetanus, N meningitidis
S pyogenes
-
7/30/2019 Microbiology Charts
17/17
Disease Associated Areas Affected
UTI, Pyelonephritis Bladder, Ureters, Kidneys
Gonorrhea, Chlamydia, Syphillis, Chancroid Genitals
Strep throat, Influenza Throat, Lungs
Pneumonia, Tuberculosis Lungs
Conjunctivitis (Infectious and Neonatal), Trachoma Eyes
Lyme disease, Rocky Mountain Spotted Fever Skin
Cholera, Salmonella food poisoning GI Tract
Botulism, Tetanus, Meningitis CNS
Sepsis, Endocarditis Heart, Blood Vessels
top related