microfluidic perfusion system for yeast microscopy · microfluidic perfusion system for yeast...

MICROFLUIDIC PERFUSION SYSTEM FOR YEAST MICROSCOPY

Bringing Microfluidics to Life www.cellasic.com | July 2010

Dynamic Solution Exposure

Contact: pjlee@cellasic.com

We developed a microfluidic plate for live cell imaging that maintains yeast in a single focal plane for long term, high magnification perfusion experiments. The plate contains 4 parallel flow units, each with 6 upstream solution inlets. The chambers accommodate cells from 4-10 micron in size.

Microfluidic Perfusion Plate

Yeast Live Cell Imaging

Philip Lee, Terry Gaige, and Paul HungCellASIC Corp., San Leandro, CA, USA

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Maintains cells and daughters in single focal plane with perfusion

Clear optics with #1.5 thickness (170 um) glass coverslide bottom

Different height traps for yeast cell size variations

Key Features

Microfluidic Plate Yeast Chambers Flow Control System

Trapping site at rest Pressure driven loading of cells

Release pressure, physical trap of cells

trap height

elastic ceiling

glass floor

Before Loading 5 Seconds at 8 psi

250 um

Cell Trapping Mechanism

Budding Yeast

Fission Yeast

Flow Rates Switch Times

6 Flow Inlets

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4.0 um Trap

5.0 um Trap

4.5 um Trap

1 mm

Yeast Chamber

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S. cerevisiae cultured in the microfluidic chamber over 10 hours. Perfusion rate of 4 ul/hr of SC complete medium at 30˚C. Cells imaged in the 4.0 um trap with a 40X objective.