not for medical diagnostic use - promega · single-base resolution spectrum compact achieves a 1bp...
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Not for Medical Diagnostic Use
The Spectrum Compact CE System is an integrated and efficient
instrument that brings you the independence to perform Sanger
sequencing and fragment analysis in your laboratory, under your
control and at your convenience.
It is designed for use with existing sequencing chemistries using
fluorescently labeled dideoxynucleotide triphosphates and 4-, 5- and
6-dye STR kits from Promega, and other commercially available kits.
Capillary Electrophoresis Just Got Personal
The Spectrum Compact is the solution for academic, clinical
research and forensic laboratories looking to take analyses into their
own hands. This innovative instrument provides a high return on
investment, conserves valuable bench space and allows flexibility in
sample batch sizes.
The Spectrum Compact CE System is:
A personal, benchtop instrument
• Conforms to your workflow
» No waiting to batch samples or send samples to an outside lab
• Enables a more efficient workflow
» 32 sample capacity using four 8-well strip tubes
» 4-capillary array
• Saves time and reduces reagent waste
Capable of Sanger sequencing and fragment analysis
• Compatible with commercially available sequencing kits
» Uses fluorescently labeled dideoxynucleotide triphosphates
» Optimized for a broad range of applications including:
• De novo sequencing
• NGS confirmation
• Resequencing
• Mutation detection
• Mitochondrial sequencing
• Designed to work with all common STR kits on the market
» Compatible with 4-, 5- and 6-dye STR chemistries
» Other uses include:
• Microsatellites
• PCR sizing
• SNP genotyping
Not for Medical Diagnostic Use
Integrated Touchscreen Operation
The Spectrum Compact Control Software provides a simple user
interface with a clear display of useful features including run set
up, consumables and array usage information, as well as system
maintenance reminders.
• Easy-to-use operational workflow wizard
• Operations run directly on the instrument touch screen
• Ability to monitor run progress and view analyzed results while a
run is in progress
• Export files are compatible with commercially available data
analysis software such as GeneMarker®, Mutation Surveyor® and
GeneMapper®.
Small-Footprint Advanced Multiplexing
The Spectrum Compact offers the high performance of a 6-dye
compatible CE instrument, but at an affordable price point.
Electropherogram of 1ng of 2800M control DNA analyzed with PowerPlex®
Fusion 6C System run on prototype Spectrum Compact CE System. Injection was
performed at 1.2kV for 8 seconds.
Electropherogram of the PowerPlex® Fusion 6C System allelic ladder run on
prototype Spectrum Compact CE System. Injection was performed at 1.2kV for
8 seconds.
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600060 100 140 180 220 260 300 340 380 420 460 500
50004000300020001000
0
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50004000300020001000
0
600060 100 140 180 220 260 300 340 380 420 460 500
50004000300020001000
0
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50004000300020001000
0
600060 100 140 180 220 260 300 340 380 420 460 500
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0
AMEL D3S1358 D1S1656 D2S441 D10S1248 D13S317 Penta E
D16S539 D18S51 D2S1338 CSF1PO Penta D
TH01 vWA D21S11 D7S820 D5S818 TPOX
D8S1179 D12S391 D19S433 SE33 D22S1045
DYS391 FGA DYS576 DYS570
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1TA
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300060 100 140 180 220 260 300 340 380 420 460 500
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AMEL D3S1358 D1S1656 D2S441 D10S1248 D13S317 Penta E
D16S539 D18S51 D2S1338 CSF1PO Penta D
TH01 vWA D21S11 D7S820 D5S818 TPOX
D8S1179 D12S391 D19S433 SE33 D22S1045
DYS391 FGA DYS576 DYS570
Not for Medical Diagnostic Use
Single-base Resolution
Spectrum Compact achieves a 1bp resolution as can be seen in the D1S1656 and
D2S441 loci in the PowerPlex® Fusion 6C System Allelic Ladder. Injection was
performed at 1.2kV for 8 seconds.
1401
2TA
2000150 170 190 210 230 250 270
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0
D1S1656 D2S441 D10S1248
A personal, affordable benchtop instrument
for Sanger sequencing and fragment
analysis applications
Accurate and Fast Sequencing
Spectrum Compact supports Sanger sequencing applications used as
orthogonal technology for verification of NGS base calls and DNA and
gene sequencing required in a variety of genomic applications.
Panel A
Panel B
Representative portion of an electropherogram (Panel A) generated using the Applied
Biosystems BigDye® v3.1 Kit, Polymer 7, and Hitachi base calling software; (Panel B)
whole data file.
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8TA
5,200
260 270 280 290 300 310 320 330
5,300 5,400 5,500 5,600 5,700 5,800 5,900 6,000 6,100
G A A G A C C C T T A C T A C T G T C C A A G G G A T C G C T G A T G A T T A C G A T A A A A A G A A A C T A G T G A A G G C G T T T A A G A A A A A G T T T G C C T G
1400
9TA
14,00013,00012,00011,00010,0009,0008,0007,0006,0005,0004,0003,0002,000 15,000
Not for Medical Diagnostic Use
Spectrum Compact Consumables and Accessories
Plug-and-play, prefilled consumables bring capillary electrophoresis
capabilities to the hands of any researcher in the laboratory regardless
of skill level or expertise.
Not only is the Spectrum Compact simple to set up and operate,
related consumables such as prefilled buffer and polymer are available
in convenient packaging. Accessories such as plates, septa mats
and formamide are also available for purchase. All consumables and
accessories use 2D barcoding to track key information.
The Spectrum Compact System Control Software enables real-time tracking of key
reagents and consumables.
Spectrum Compact Consumables
Spectrum Compact Capillary Array
• 4-Capillary, 36cm
• Barcoded for consumable tracking
Spectrum Compact Cathode Septa Mat
• 8-well septa mat specific for the Spectrum Compact cathode
buffer cartridge
Spectrum Compact Polymer4
• 64 samples
• Barcoded for consumable tracking
Spectrum Compact Polymer7
• 64 samples
• Barcoded for consumable tracking
Spectrum Compact Plate Base & Retainer, 32-Well
Spectrum Compact Buffer
• Prefilled, ready-to-use anode and cathode cartridges
• Barcoded for consumable tracking
Spectrum Compact Wash Solution
• Ready-to-use, prefilled syringe for system washes
• Barcoded for consumable tracking
Universal Consumables
Strip Cap, 8-Well
• 8-strip domed caps for PCR plates and tubes to prevent
evaporation and contamination
• Certified RNase- and DNase-free
Strip Tubes, 8-Well
• 8 strip 0.2ml PCR tubes
• Certified RNase- and DNase-free
Strip Septa Mat, 8-Well
• Autoclavable and reusable up to 5 times for a more cost-
effective alternative to other sealing methods
Formamide, CE-Grade, 25ml
• Highly deionized, low conductivity, <5μS
• Used for electrokinetic injection on CE instruments
Not for Medical Diagnostic Use
» 4-capillary array
» Polymer
» Anode and cathode buffers
» Wash solution
» Cathode septa mats
» Plate bases and retainers
» Formamide
» Reagents necessary for installation
Promega Support
Promega chemistries and Spectrum Compact are provided as an
integrated system for delivering optimized results for sequencing and
fragment analysis. On-site installation and training for the instrument,
software and kits is included with the purchase of the instrument.
Additionally, flexible service and support options will be available,
depending on the requirements of your laboratory.
Promega offers service and support plans for preventative
maintenance, on-site service and validation plans to ensure minimal
interruption of your laboratory operations.
• Phone-based and on-site consultation
• On-site bench work tailored to your requirements
• Rapid turnaround of data analyses and summary reports
System Components
The Spectrum Compact CE System is supplied as follows:
• Capillary electrophoresis instrument
• Integrated touchscreen
• On-site service for installation and initial user training
Dimensions 40cm W x 70cm D x 60cm H
Weight 41kg
Main Power Voltage 95-240VAC, 50-60Hz (global compatibility)
15 amps
Operating Environment 15°C – 30°C
Sample Capacity 1-32 samples (four 8-well strip tubes)
Injection Throughput 4-capillary array
Capillary Array Length 36cm
Service and Warranty Service installation
On-site application training
Extended service contracts will be available separately–please inquire.
Validation Support Available separately–please inquire.
Expected Specifications
40cm
60cm
70cm
Not for Medical Diagnostic Use
For Research Use Only. Not for use in diagnostic procedures.
PowerPlex is a registered trademark of Promega Corporation.
GeneMarker and Mutation Surveyor are registered trademarks of SoftGenetics.
ThermalSeal is a registered trademark of Excel Scientific, Inc.
GeneMapper is a registered trademark of Thermo Fisher Scientific.
Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.
ALL PRODUCTS, PRODUCT SPECIFICATIONS AND DATA ARE SUBJECT TO CHANGE WITHOUT NOTICE TO IMPROVE RELIABILITY, FUNCTION OR DESIGN OR OTHERWISE.
© 2017 Promega Corporation. All Rights Reserved. Printed in USA 2/17. 34717955. Part #BR292
To learn more, visit: www.promega.com/SpectrumCompact
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