phase contrast images contrast microscopynsmn1.uh.edu/mrea/omnb 08/omnb 08 lec 4web.ppt.pdf ·...

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Contrast Microscopy

Koehler Illumination

Dark Field

Phase Contrast

Differential Interference Contrast

Hoffman Modulation

Phase Contrast Images

Brightfield Phase Contrast

Phase Contrast MicroscopyF. Zernike, 1930s

Phase Contrast Microscopy

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Phase Contrast Images

Brightfield Phase Contrast

Contrast Microscopy

Koehler Illumination

Dark Field

Phase Contrast

Differential Interference Contrast

Hoffman Modulation

DIC (Nomarski) Images

Phase Contrast Nomarski Optics

James Maxwell(1831-1879)

Electromagnetism

ElectromagneticTheory of Light

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Wave Nature of Light

Under some conditions, light behaves as an electromagnetic wave(Maxwell, 1855). In this model, electric and magnetic fields oscillateperpendicular to one another and to the direction of propagation of thelight wave.

Refractive Polarization

David Brewster(1781 - 1868)

Brewster Angle[53o - 56o]

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Optical Birefringence

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Differential Interference ContrastMicroscopy

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DIC

PhaseContrast

PhaseContrast

DIC

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Hoffman Modulation

Hoffman Modulation Images

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