potable biosensor
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BioDevice Technology, Ltd. BDT is a company born from Japan Advanced Institute of Science and Technology (JAIST) and was established in July 2003 by Professor Eiichi Tamiya and five faculties.
High Sensitive Immunochromatography PCT/JP2006/323617
DEP-Chip series for electrochemical biosensors
Electrochemical DNA sensors (JP3642574)
Gold-Linked Electrochemical Immuno Assay GLEIA, PCT/JP2007/56992)
Our Objects of Establishment Establish products as fruits of research in
universities. Back up of making collaboration academic
researchers with companies. Build innovation by collaboration among
academic, companies and government
R & D of Biosensors
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Disposable Type Screen Printed Electrodes
DEP-Chip Series
SP-N, SP-P (carbon)
SR-N (gold)
Square working electrode
PCR-P01
EP-N (carbon) EP-PP (carbon, WE with ring and dam) ER-N (gold)
Round working electrode
One drop test Adapted size for PCR tube mass production/disposal/low cost Possible customized fabrication
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Pathogenic bacteria/virus Salomonella, Ecoli O-157, Hepatis virus B, Influ virus Food safety GMO, Meat quality SNPs ApoE, ADH
Printable electrodes based biosensors
Gene sensing
Enzyme activity sensing
Gold Linked Electrochemical Immuno Assay : GLEIA hCG hormon : 36 pg/mL (612 fM) insulin : 125pg/mL
Antibody sensing
Acetylcholine esterase inhibition Organophosphate pesticide : 0.01 ppm HIV protease inhibition
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Original principle of DNA sensor Japanese patent
A B
(Low concentration of DNA
or Before amplification)
(High concentration of DNA
or After amplification)
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00.2
0.4
0.6
0.8
1
100 200 300 400 500 600 700
0
0.5
1
1.5
2
2.5
100 200 300 400 500 600 700
a
b
c
d
e
0
0.5
1
1.5
2
2.5
200 300 400 500 600 700 800
a
b
c
M a b c d e
M a b c
M a b c
0
0.4
0.8
1.2
1.6
2
200 300 400 500 600 700 800
a
b
c
M a b
Salmonella invA gene (PCR)
O157 gene (LAMP)
negative
positive
Genetically-modified corn (LAMP)
Hepatitis B virus gene from
blood sample (Nested PCR)
negative
positive
negative
negative
positive positive
Examples of various DNA targets detected
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Analyst,136, 2064 (2011)
Rapid detection for screening of influenza A virus:
microfluidic RT-PCR chip and electrochemical DNA sensor
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Analyst, 2011, Advance ArticleDOI: 10.1039/C1AN15638A
Semi-real time electrochemical monitoring for influenza virus RNA by
RT-LAMP using USB powered portable potentiostat
BDT miniSTAT BDT DEP chip
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Gold nanoparticle redox signal enhancement for detection of antigens
After the recognition reaction between the surface-immobilized primary antibody and hCG, the captured antigen
was sandwiched with a secondary antibody that was labeled with gold-nanoparticles. Then the amount of
gold nanoparticles was determined by its redox signal.
The primary antibody was Immobilized directly on the working electrode, and a sandwich-type immuno-reaction was performed
A high potential was applied In HCl for the oxidation of gold nanoparticles
DPV* : differential pulse voltanmetry
The voltammetric measurement was performed.
References Direct fabrication of catalytic metal nanoparticles onto the surface of a screen-printed carbon electrode, Electrochemistry
Comunications 8, 1375 (2006).
Gold nanoparticle-based redox signal enhancement for sensitive detection of human chorionic gonadotropin hormone Electroanalysis 20,14 (2008)
Gold-Linked Electrochemical Immuno-Assay (GLEIA, PCT/JP2007/56992)
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hCG detection by GLEIA
Sample vlume:1 1.5L Lower detection limit: 62.5 pg/mL
Human Chorionic Gonadotropin Hormone standard curves
constructed with buffer () and with serum ()
Potential (V)
0pg/ml
62.5pg/ml
125pg/ml
250pg/ml
500pg/ml
1000pg/m
Typical differential pulse
voltammograms of GLEIA.
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Insulin detection by GLEIA
Insulin standard curve constructed with human serum
Sample vlume:1.4L Lower detection limit: 125 pg/mL
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HOMA-R fasting blood sugar level (mg/dL) fasting blood insulin level (U/mL)
Application of GLEIA
HOMA-R measurement equipment
HOMA-R is a marker of insulin resistance, metabolic syndrome and diabetics. It is an useful marker to prevent disease progression. We have been developing HOMA-R measurement equipment with the support of Ministry of Economy, Trade and Industry. Blood insulin levels are measured by GLEIA and TAS technologies.
A prototype model of HOMA-R measurement equipment
Measurement chips for insulin and blood sugar
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Portable multichannel electrochemical system
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Pregnancy, cancer, infectious disease, cardiovascular problems etc.
Clinical Diagnosis
Food safety test
Allergens, food toxins, GMO
Ecological detection
Endocrine disturbing chemicals
Immuno-chromatographic Test Strip and applications
Marketplace in Japan(2006)
$250 million
Influenza diagnosis kits
in Japan
$110 million
Problem Relative low sensitivity around 1 ng/mL Semi-quantitative
Advantage User friendly format, easy to use Rapid to obtain test result Less interface due to chromatographic separation Relative low cost
Suit with screening or POC tests
Applications
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Normal strip Enhancer-based strip
Enhancement Assay for Immunochromatographic test strip
PCT/JP2006/323617
Apply sample solution to a
conjugation pad
Antigens react with nanogold-
labeled antibodies and
complex flow to capture antibodies
Conjugation pad Test line
Control line Absorption pad
Capture antibody on test line
Antibody for control line Nanogold labeled antibody
antigen
Antigen-antibody
complex
flow
The complex of antigen and nanogold labeled antibodies accumulated and a higher amount of
gold nanoparticles in close proximity were brought in the enhanced test strip. As a result, higher
Intensity of the localized surface plasmon resonance absorbance of the nanogold particles are
able to obtain and a distinct red color can be easily observed.
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Enhancement of sensitivity
Normal and the enhancer-based strips for the detection of hCG in PBS
0
50
1000 100 ontrol
( 1
Normal strip
40
30
20 Inte
nsi
ty
10
10 1 Concentration of hCG (pg/ml)
Enhancer-based strip
In PBS
The lower limit of detection was 100pg/ml
in the normal strip, whereas it was 1pg/ml
in the enhancer-based strip.
pg/ml pg/ml
Normal strip Enhancer-based strip
Test line
Control line
1000 1000 10 10 Control Control
100 1 1 100
In serum
The lower limit of detection was 100pg/ml
in the normal strip, whereas it was 10pg/ml
in the enhancer-based strip.
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Production list
Object Species Significance Lower limit of
detection
PSA human Prostate cancer marker 2 ng/mL
hCG human Pregnancy maker 1 pg/mL
Insulin human Metabolic syndrome marker 125 pg/mL
Hemoglobin human Gum disease marker 30 ng/mL
HbA1c human Blood glucose level marker 0.25 ng/mL
Ig E human Maker of allergy to cedar
pollens, mite and cat hair Level 2
sIgA canine Stress marker 20 ng/mL
Cortisol human Stress marker 8 ng/mL
Testosterone human Climacteric marker 125 pg/mL
E. coli E. coli Microbial contamination OD600 0.3
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Environmental protection heavy metal
pesticide,herbecide
VOC, odorants
endocrine disruptors
waste water control
pathogenic bacteria
Security explosives
bio/chemical weapons
criminal trace
Food production control taste (tongue, nose)
freshness
key chemicals
(sugar, amino acids etc.)
fermentation control
Food safety pesticide, herbecide
pathogenic bacteria/virus
BSE, chicken flu virus
carcinogens, allergy, GMO
Agriculture/ identification of breed
(rice, wagyu, tuna--- )
seed preservation
(virus free--)
soil and compost test
Bioelectronics/energy biotransistor
biocomputer
biobattery
sustainable system
Drug screening bioassay
DNA/protein chip
alternative to animal tests
Medical diagnosis biochemical tests
cancer
life-style disease
allergy
gene
pathogens
Healthcare mental stress
rapid tests for
blood/urine
point of care
testing
Biosensors application for
medical/environmental/food monitoring
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Related Publications 1)Electrochemical DNA quantification based on aggregation induced by Hoechst 33258, M.Kobayashi, T.Kusakawa, M.Saito, S.Kaji, M.Oomura, S.iwabichi, Y.Morita, Q.Hasan and E.Tamiya Electrochemistry Communications 6(4) 337-343(2004) 2)Electrochemical coding of single nucleotide polymorphisms by monobase modified gold nanoparticles, K.Kerman, Y.Morita, Y.Takamura, M.Ozsoz, E.Tamiya, Analytical Chemistry, 76, 1877-1884 (2004) 3)Recent trends in electrochemical DNA biosensor technology(Review), K. Kerman, M. Kobayashi, E.Tamiya, Meas.Sci.Technol. 15, R1-R11 (2004) 4)Electrochemical genosensor for the rapid detection of GMO using loop-mediated isothermal amplification, Minhaz Uddin Ahmed, Masato Saito, M. Mosharraf Hossain, S. Ramachandara Rao, Satoshi Furui, Akihiro Hino, Yuzuru Takamura, Masahiro Takagi and Eiichi Tamiya, Analyst, 134, 966-972 (2009) 5)Nanomaterial-based electrochemical biosensors for medical applications, Kagan Kerman, Masato Saito, Eiichi Tamiya, Shohei Yamamura, Yuzuru Takamura, Trends in Analytical Chemistry, 27(7) 585-592(2008) 6)Electrochemical Biosensors for Medical and Food Applications, Minhaz Uddin Ahmed, Mohammad Mosharraf Hossain, Eiichi Tamiya, Electroanalysis, 20(6), 616-626, 2008 7)Electrochemical DNA biosensor using disposable electrochemical printed (DEP) chip for the detection SNPs from unpurified PCR amplicons, Minhaz Uddin Ahmed, Koutarou Idegami, Miyuki Chikae, Kagn Kerman, Piyasak Chaumpluk, Shouhei Yamamura and Eiichi Tamiya Analyst 132, 431-438 (2007) 8)Electrochemical Genosensor Based on Peptide Nucleic Acid-Mediated PCR and Asymmetric PCR Techniques: Electrostatic Interactions with a Metal Cation, Kerman, K.; Vestergaard, M.; Nagatani, N.; Takamura, Y.; Tamiya, E., Analytical Chemistry, 78(7), 2182-2189 (2006) 9)Novel electrochemical identification and semi quantification of bovine constituents in feedstuffs, P. Chaumpluk et al., Science and Technology of Advanced Materials 7 (2006) 263269 10) Direct fabrication of catalytic metal nanoparticles onto the surface of a screen-printed carbon electrode, Electrochemistry Comunications 8, p1375-1380 (2006). 11) An electrochemical approach for the detection of HIV-1 protease, K. Kerman et al. The Royal Society of Chemistry 2007 Chem. Commun., 2007, 38293831 12) Accumulation of amplified target DNAs using thiol/biotin labeling, S1 nuclease, and ferrocenestreptavidinmagnetic system and a direct detection of specific DNA signals with screen printed gold electrode, P. Chaumpluk et al., Science and Technology of Advanced Materials 8 (2007) 323330 13) Amyloid- detection with saccharide immobilized gold nanoparticle on carbon electrode, M. Chikae et al., Bioelectrochemistry 74 (2008), 116-123 14) Gold nanoparticle-based redox signal enhancement for sensitive detection of human chorionic gonadotropin hormone, Koutarou Idegami et al., Electroanalysis 20, (2008), 14-21 15) Semi-real time electrochemical monitoring for influenza virus RNA by reverse transcription loop-mediated isothermal amplification using a USB powered portable potentiostat,N. Nagatani et al., Analyst, DOI: 10.1039/c1an15638a (2011)
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