procedure for canine major* crossmatch. canine only · 5 clean test tubes (5ml) n°2: xm test...

5 Clean test tubes (5ml)

N°2: XM TEST PROCEDURE

1 2

4With blood

collector stripIn the clean test tube

1Donor’s tube

ORDonor’s blood bag segment

Bu�er

EDTA

D

Additional material needed:1 centrifuge

5 XM membranes

1 Wash Buffer

1 white top buffer 1

5 Blood collector strips

5 Small pipettes

Material provided:

5 Large pipettes

1 green top buffer 2

1 2

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Canine Crossmatch TestStore at room temperature

Bu�er Bu�er WASH BUFFER

Procedure for canine major* crossmatch. CANINE ONLY

N°1: PREPARATION OF BLOOD SAMPLES

Recipient Donor

Serum or plasma

pRBCs(packed Red Blood Cells)

• EDTA• ACD

Do not use Heparin

• CPD• Plain tube

Centrifuge blood tube in order to collect :

Centrifuge blood tube.Discard the serum or plasma to collect pRBCs.

OR

Collect blood from blood bag segment.

pRBCs

R D

(MAJOR = Donor RBC + Recipient serum or plasma)

* For minor XM reverse the blood samples : Minor = Donor Serum / Plasma + Recipient RBC and perform the same procedure

3 4

5

6 7 8

Up to 5min

Recipient’s tube

Mix gently & incubate 10’ at room temperature

Suspension washing procedure

A B C

3ML

With the large pipette

2

2Bu�er

EDTA

R

Add

Insert the XM membrane.

WASH BUFFER

With the small pipette

3

of serum or plasma in

the test tube

Suspension washing procedure STEP 5 MUST BE DONE 3 TIMES

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Read the result

10min

C

2min

Don’t forget the balance tube

Discard the supernatant

Centrifuge

1000 g

Mix gently

Mix gently

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Incompatible XM + Compatible XM -RESULT

Date :

Name/N° of recipient :

Lab Test lot number :

Name/N° of donor :

Test performed by :

TO INTERPRET YOUR RESULT, STICK THE MEMBRANE BELOW

+ -IncompatibilityDO NOT TRANSFUSE

CompatibilitySAFE TRANSFUSION

XM= CROSSMATCH TEST C = CONTROL LINE

RESULT FORM CANINEfor Crossmatch Test (XM)

Weak line= positive result

INTERPRETATION OF RESULTS

Read immediately after testing

XMC

!In case of any other result, please take a picture and send us an email at : contact@alvedia.com

White line= negative result