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QUALITY CONTROL RECORDS
National Society for Histotechnology 4201 Northview Drive, Suite 502
Bowie, Maryland 20716-2604 Phone: 301-262-6221 Fax: 301-262-9188
email: histo@nsh.org website: www.nsh.org
Quality Control Record sheet
Tissue Specimen
Date
Pathology #
Specimen
# Pieces/ Cassette
# H&E
# Special Stains
Pathologist/ Technician
8/18/86
S86-2001
Skin-Leg
1
1
3
DC/AF
“
S-86-2002
Liver-bx
1
1
5
“
“
S86-2003
T & A
1
1
0
“
“
S86-2004a
Uterus
1
1
0
“
“
S86-2004b
Tubes
2
1
0
“
“
S86-2004c
Ovary
1
1
0
“
1
Quality Control Record sheet
Tissue Specimen
Date
Pathology #
Specimen
# Pieces/ Cassette
# H&E
# Special Stains
Pathologist/ Technician
2
Quality Control Sheet
Slide Evaluation Checklist
Slide ID# __________Kidney – 43 ____________ Preparer__________AF___________________ Date ___________9/5/86____________________ Evaluator _______DD2___________________
SLIDE APPEARANCE ACCEPTABLE NOTACCEPTABLE COMMENTS
Label position X
Label Information X
Coverslip position X
Mounting medium X-none
Slide (colored) X
Tissue section position X-none
Air bubbles X-none
PROCESSING ARTIFACTS Fixation X
Decalcification NA NA = Not Applicable
Fixative pigments X-none
Cell shrinkage X-none
Others
MICROTOMING
Thickness
Knife Lines X-none
Incomplete X
Floaters few
Compression/Folds minor
Others
STAINING Nuclear Stain X
Cytoplasmic stain X Collagen & smooth muscle not distinguishable
Special stain element
Counterstain
Staining artifacts
3
Quality Control Sheet Slide Evaluation Checklist
Slide ID # _________________________________ Preparer __________________________________
Date _____________________________________ Evaluator _________________________________
SLIDE APPEARANCE ACCEPTABLE NOT ACCEPTABLE COMMENTS
Label Position
Label Information
Coverslip position
Mounting medium
Slide (colored)
Tissue section Position
Air bubbles
PROCESSING ARTIFACTS
Fixation
Decalcification
Fixative pigments
Cell shrinkage
Others
MICROTOMING
Thickness
Knife Lines
Incomplete
Floaters
Compression/Folds
Others
STAINING
Nuclear Stain
Cytoplasmic stain
Special stain element
Counterstain
Staining artifacts
4
Quality Control Record sheet: Automatic Tissue Processor
Type of Processor: Closed Week of: August 17, 1986
Station # Day) Solution Date) Runs/Day)
8/18 M
__1__
8/19 Tu
__1__
8/20 W
__1__
8/21 Th
__2__
8/22 F
__1__
8/23 Sa
__0__
_____ Su
_____ 1 Rotate/Discard NFB Qc Check
New Ph-7
Discard Discard
2 Rotate/Discard NFB QC Check
New Ph-7
Rotate- New
Rotate- New
3 Rotate/Discard 80% alc QC Check
New 80%
Discard- New
Rotate- New
4 Rotate/Discard 95% alc QC Check
New 95%
Discard Discard
5 Rotate/Discard 95% alc QC Check
New 95%
Rotate- New
Rotate- New
6 Rotate/Discard 100% alc QC Check
New 100%
Discard Discard
7 Rotate/Discard 100% alc Qc Check
New 100%
Rotate Rotate
8 Rotate/Discard 100% alc QC Check
New 100%
Rotate New
Rotate New
9 Rotate/Discard xyl QC Check
New Discard Discard
10 Rotate/Discard xyl QC Check
New Rotate- New
Rotate- New
11 Rotate/Discard Paraffin QC Check
New
12 Rotate/Discard Paraffin QC Check
New
13 Rotate/Discard ______ QC Check
Purge 1 Rotate/Discard xyl
Used 2X
Used 2X
Used 2X
Used 4X Discard
New Used 2X
Purge 2 Rotate/Discard 100% alc
Used 2X
Used 2X
Used 2X
Used 4X Discard
New Used 2X
Initials
AF
AF
AF
AF
DD
5
Quality Control Record sheet: Automatic Tissue Processor
Type of Processor _____________________________ Week of ____________________________
Station # Day) Solution Date) Runs/Day)
M
_________
T
_________
W
__________
TH
__________
F
__________
Sa
__________
Su
_________
1 Rotate/Discard ________ QC Check
2 Rotate/Discard ________ QC Check
3 Rotate/Discard ________ QC Check
4 Rotate/Discard ________ QC Check
5 Rotate/Discard ________ QC Check
6 Rotate/Discard ________ QC Check
7 Rotate/Discard ________ QC Check
8 Rotate/Discard ________ QC Check
9 Rotate Discard ________ QC Check
10 Rotate/Discard ________ QC Check
11 Rotate/Discard ________ QC Check
12 Rotate/Discard ________ QC Check
13 Rotate/Discard ________ QC Check
Purge 1 Rotate/Discard ________
Purge 2 Rotate/Discard ________
Initials
6
Quality Control Record sheet: Decals
Decal Solution: 5% Formic Acid
Date Pathology #
Fixative Decal Started
Decal Ended
Comments/Initials
6/24/86 A86-102 NBF 6/25/86 6/27/86 Decal charged 6/26/86 Cut OK AF
7/01/86 S86-212 NBF 7/01/86 7/02/86 Tissue gritty while microtoming AF
7
Quality Control Record sheet: Decals Decal Solution: _________________________________
Date Pathology #
Fixative Decal Started
Decal Ended
Comments/Initials
8
Quality Control Record sheet: Routine Stain Solutions
Week of: August 17, 1986 D – Discard F – Filtered N – New R – Rotated
Day) Solution Date) Slides/Day)
8/18 M
200
8/19 T
150
8/20 W
100
8/21 Th 30
8/22 F
100
8/23 Sa 0
Su
_____ Rotate/Discard xyl
N D D D D
Rotate/Discard xyl
N R R R R
Rotate/Discard xyl
N R/N R/N R/N R/N
Rotate/Discard 100%
N D D D D
Rotate Discard 100%
N R R R R
Rotate/Discard 100%
N R/N R/N R/N R/N
Rotate/Discard 95% QC Check
N - - - -
Hema- Rotate/Discard toxylin QC Check
N F Ph 2.8
F Ph 2.8
F Ph 2.8
F Ph 2.8
Rotate/Discard Acid alc QC Check
N N N
Rotate/Discard Blueing QC Check
N N N
Rotate/Discard 95% QC Check
N - -
Rotate/Discard Eosin QC Check
N F Ph 4.5
F Ph 4.5
F Ph 4.5
F Ph 4.5
Rotate/Discard 100% QC Check
N D D - D
Rotate/Discard 100%
N R R - R
Rotate/Discard 100%
N R/N R/N - R/N
Rotate/Discard xyl
N D D - D
Rotate/Discard xyl
N R R - R
Rotate/Discard xyl
N R/N R/N - R/N
Initials
AF
AF
AF
AF
AF
9
Quality Control Record sheet: Routine Stain Solutions Week of: _________________________________________ D – Discard, F – Filtered, N – New, R – Rotated
Day) Solution Date) Slides/Day)
M
______
T
______
W
______
Th
______
F
______
Sa
______
Su
______ Rotate/Discard __________
Rotate/Discard __________
Rotate/Discard __________
Rotate/Discard __________
Rotate/Discard __________
Rotate/Discard __________
Rotate/Discard __________ QC Check
Rotate/Discard __________ QC Check
Rotate/Discard __________ QC Check
Rotate/Discard __________ QC Check
Rotate/Discard __________ QC Check
Rotate/Discard __________ QC Check
Rotate/Discard __________
Rotate/Discard __________
Rotate/Discard __________
Rotate/Discard __________
Rotate/Discard __________
Initials
Quality Control Record sheet
Special Stain for
Control Block ID S86-32 Date 8/06/86 to
Date
Pathology #
Source
Fixative
Control
+ / -
Unknown
+ / -
Technician
8/06/86 S-86-786 Liver NBF + + AF
8/08/86 S-86-807 Bone Marrow Bouins + + AF
8/08/86 S-86-811 Colon (tumor) NBF + - AF
11
Quality Control Record sheet
Special Stain for
Control Block ID Date to
Date
Pathology #
Source
Fixative
Control
+ / -
Unknown
+ / -
Tehnician
12
Quality Control Record sheet: Temperature Control
Month/Year July, 1986
Instrument: Acceptable Range:
DATE
Slide Dryer
58-62° C
Oven
35-39° C
Embedding Center
58-62° C1 60° 37° 60°
2 60° 37° 60°
3 60° 37° 60°
4 - 37° -
5 - - -
6 - - -
7 59° - 61°
8 60° 37° 61°
9 59° 37° 61°
10 59° 37° 61
11 37°
12
13
14
15 60° 37° 61°
16
17
18
19
20
21
22
23 60° 37° 60°
24
25
26
27
28
29
30 60° 37° 60°
31
13
Quality Control Record sheet: Temperature Control
Month/Year
Instrument: Acceptable Range:
DATE
Slide Dryer
_______________
Oven
_______________
_______________
_______________
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
14
Quality Control Record sheet Procedure Manual Changes
Effective
Date Problem/Procedure Addition/Correction Implemented Initials
6/23/86
Tissues too hard: crunchy
Change station 3 on tissue processor from 95 % alcohol to 80 % alcohol
AF
Results: Better. Fibrous tissues still difficult to cut
6/24/86
Fibrous tissue hard Change station 3 on tissue processor from 80 % alcohol to 70 % alcohol
AF
Results: Fibrous tissues - OK
7/11/86
Eosin too dark Rinse in 95 % alcohol for 1 minute, Post eosin
AF
Results: OK. Slides lighter in eosin
15
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