raav2-cont shrna
Post on 05-Jan-2016
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Supplemental Fig. 2. rAAV2-Cont shRNA. a. b. 1. rAAV2-Cont shRNA (Cont shRNA ) 2. rAAV2-mTOR shRNA ( mTOR shRNA ). rAAV2-mTOR shRNA. pH1. pCMV. GFP. pol A. shRNA. Bi-directional promoter. 4 days. 2 days. d. Cont. mTOR. Cont. mTOR. c. mTOR. P- mTOR (S2448). *. - PowerPoint PPT Presentation
TRANSCRIPT
rAAV2-Cont shRNA
rAAV2-mTOR shRNA
mTOR
P-mTOR(S2448)
P- p70S6K(T389)
GFP
actin
Cont mTOR Cont mTOR
2 days 4 days
1. rAAV2-Cont shRNA (Cont shRNA)2. rAAV2-mTOR shRNA (mTOR shRNA)
Bi-directional promoter
shRNA pH1 pCMV GFP pol A
a
c
b
d
2D 4D0.0
0.2
0.4
0.6
0.8
1.0
1.2
Cont shRNA mTOR shRNA
Re
lati
ve
am
ou
nt
of
mT
OR
R
NA
ex
pre
ss
ion
Supplemental Fig. 2
*
**
P-mTOR(S2481)
P- AKT(S473)
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