sable’s scope who we are founded by john r.b. lighton, ph.d. 25 years of scientific discovery...

SABLE’S SCOPEWho We Are

• Founded by John R.B. Lighton, Ph.D.• 25 years of scientific discovery• Tools for understanding – with a respirometry focus• Scientists and engineers still active in research• Growing at 25% for last 4 years• Growing at over 45% in 2011• Fueled by Promethion

SABLE’S GLOBAL INFLUENCE

Sable’s Promethion InspirationCUSTOMER REQUESTS:

Turnkey Solution

Fully Integrated

Live-in Cages with Bedding

Fully Traceable Data

Running Wheel

Expertise

High Throughput

Scalable

Accurate

User Friendly

No air tight seal

Good Customer Service

Customizable Analysis

No Chemical Desiccants

MARKET RESEARCH: Competition

• Black box calculations• No raw data storage or data traceability• Customer service• Difficult to use• Very complex to set up

The Good

• Widely used and cited• Integrated system• High Throughput

The Bad

• Small, not live-in cages• No bedding in cages• Very low flow rates (IACUC)• Accuracy concerns• No integrated running wheels

The Ugly

METABOLIC SCREENING

EXISTING CUSTOMERS

THE CAGE…

FOOD INTAKE

Food Intake Monitor

Features

• 3mg resolution• Crumb tray reduces caching• Real-time measurement

• Universal Load Cell• Mouse or Rat hopper

Advantages

• Increased accuracy, versatility• Fine granularity gives insight into behavioral patterns

FOOD INTAKERaw Feeding Data

• Stores hopper mass vs. time• Recorded to disc at 1Hz

One Feeding Episode

• Analyze behavior• Determine individual feeding episode intake rates

FOOD INTAKESummarized Feeding Data

• Identify trends and outliers• Can zoom into any event• Automated uptake analysis• Automated meal analysis

One Feeding Episode

• Analyze behavior• Unprecedented detail available – but only if required

FOOD INTAKEProcessed Feeding Data

• Outputs cumulative intake vs. time• Shows rate of intake, structure• Can output binned data• Integrates with other cage data• Fully traceable

Food Selection

• Determine food preference• Control access to either/both foods• Integrates with other cage data

FOOD INTAKE ANALYSIS

FOOD ACCESS CONTROL

Access Control Module

Features

• Computer controlled access to food• Available for Mouse or Rat food hoppers• Intelligent Obstruction Detection

• Access Control Door• Connects to mass sensor

Advantages

• Reduced technician interaction • Customizable assays (paired/yoked)

FOOD ACCESS CONTROLUser Friendly Setup

• Select from 8 preset assays• Customize time and duration• Individual cage setup

Select Access Control Type

• Add to your custom assay• Unlimited number of variations

WATER INTAKE

Water Intake Monitor

Features

• 3 µL resolution• Real-time measurement• Stainless steel case (not shown) reduces animal interaction• Custom sipper tube reduces leakage

• Universal Load Cell• Mouse or Rat dispenser models

Advantages

• Increased accuracy• Increased granularity gives insight into behavioral patterns• Use existing sterilizing systems

WATER INTAKERaw Drinking Data

• Stores bottle mass vs. time• Recorded to disc at 1Hz

Processed Drinking Data

• Analyze behavior• Mathematical drift elimination

• Determine individual episode intake rates• EthoScan Compatible

BODY MASS

Body Mass Monitor

Features

• 3mg resolution• Real-time measurement

• Universal load cell• Mouse or Rat models available

Advantages

• Highly accurate• Provides in-cage enrichment• Reduced technician interaction

BODY MASSRaw Body Mass Data

• Stores bracket mass vs. time• Recorded to disc at 1Hz• Is used about every 15 minutes

Zoomed In

• Track body mass over time

BODY MASSProcessed Body Mass Data

• Stores most recent body mass for each timestamp• Synchronized with other data

• Diurnal cycle clearly visible

VOLUNTARY EXERCISE

Wheel-M or Wheel-R

Features

• Real-time measurement• Stainless steel construction• Entirely in cage

• Mouse or Rat Wheel• Magnetic Reed Switch• Optional: Wheel Stop

Advantages

• Increased granularity gives insight into behavioral patterns• Correlates with metabolic data

VOLUNTARY EXERCISERaw Running Wheel Data

• Stores RPS vs. time• Recorded to disc at 1Hz

Processed Running Wheel Data

• Shows cumulative distance run• Can be binned and synchronized with metabolic data

TOTAL ACTIVITY

BXYZ-M or BXYZ-R

• XY and Z IR arrays• Mounting bracket

Features

• 0.25cm calculated centroid• Real-time measurement• Intelligent obstruction detection• Rearing captured with Z axis

Advantages

• Highly accurate• Not affected by static obstructions• Correlated with metabolic data

TOTAL ACTIVITYActivity Analysis

• Total Activity• Total Distance Traveled• Rearing• Activity associated with in-cage devices• In cage position vs. time• Customizable assays• Position histograms

Activity Comparison

• WT vs. ob/ob• Interaction with FH-MB• Time spent time spent near food hopper

CALORIMETRY

Flow Generators & Gas Analyzers

• Automated calibration • Sequential or continuous monitoring• Can group for 4, 8,16, 24, 32 etc. cages• Expandable and modular• Water vapor dilution correction• Integrated O2, CO2, WVP analyzers

• Pull-mode (negative pressure)

DATA ANALYSIS Data Synchronization

• Overlay metabolic data with other continuously monitored parameters

Further Analysis

• All data are perfectly synchronized and can be exported to other programs if required (open formats)

DATA ANALYSIS Further Data Synchronization

•Body mass and food intake

Advantages

• Synchronized data yields traceability• Real time measurement improves visibility• Both enable behavioral monitoring

•Body mass and water intake

•Body mass, food intake, water intake, and wheel revolutions

DATA ANALYSIS Multiplexed vs. Continuous

• Continuous measurements give unprecedented detail• Multiplexed measurements are adequate for many research topics• You have the choice

TOTAL ACTIVITYActivity Analysis (II)

• Activity within user-defined areas of any shape• Regions of interest interactively defined on customizable background• Correlate with other data streams as needed

TOTAL ACTIVITYActivity Analysis (III)

TOTAL ACTIVITYActivity Analysis (IV)

DATA ANALYSIS

DATA ANALYSIS

DATA ANALYSIS

Multiplexing errors- more information

Error from multiplexing

Over and underestimates

DATA ANALYSISRaw Data Synchronization - Zoomed

• Body mass (blk), food intake (red), wheel (yel), and water intake (blu)

ETHOSCANBehavioral Monitoring

• All cage devices continuously monitored (“focal animal”)• Interactions sequentially processed in data analysis• Output is timestamp, position, duration, amount and activity

ETHOSCAN

Behavioral Transition Matrix

• Statistical analysis of interaction with in-cage devices• Shows probability of behaviors in the top row happening immediately after the behavior in the leftmost column

ETHOSCANTime Budgets

• Shows percentage of time spent exhibiting a specific behavior• Customizable to your cage setup and behaviors you wish to monitor

Ambulatory Budgets

• Two wild type mice –with and without access to a wheel• Note the significant difference in distance travelled

ROOM CALORIMETRY

ROOM CALORIMETRY• Time-consuming to set up and test• Just one span per run… But analyzers drift• Propane burn validations may require back-calculation of

span gases (black box!)• Cannot adequately account for water vapor dilution,

especially at low WVP• System integration difficult• Many individual instruments = many failure points and

many companies to deal with• Little or no expandability or modularity

ROOM CALORIMETRY

ROOM CALORIMETRY• Raw data not preserved, so - Not auditable• Comparisons between systems problematic• Little emphasis on traceability (pre-Jon Moon)• But – traceability alone will not help• Also need transparency!• Custom “black box” software is proprietary• Software is a priori optimized for

distinguishing between two activities

ROOM CALORIMETRY• Highly stable O2, CO2 analyzers required

• Low noise (for response correction)• WV dilution is a problem…• Chemicals are impractical…• Typically, chilled condensers are used• But these only “dry” the air to ~650 Pa WVP• At dewpoints < 1 C, they have no effect at all• Plus, FiCO2 changes…

ROOM CALORIMETRY

FiCO2 changes…

ROOM CALORIMETRY +

• Minimize O2 drift (SSI O2 analyzers)

• Then, eliminate O2 drift by repeated, interleaved FiO2 measurement (PP)

• For CO2, use interleaved SSI analyzers (PP)

• For eliminating WV dilution, don’t scrub/condense – calculate from WVP, BP! (PP)

• Record all raw data in complete detail

ROOM CALORIMETRY +• Use computational processing to reassemble

the dry O2 and CO2 data from the interleaved O2, CO2, WVP data, plus BP (PP)

• Apply rigorously neutral smoothing and response correction to data

• All using a traceable, auditable, shareable script

• Because raw data are stored, any kind of response correction you wish can be applied!

PROMETHION

PROMETHION

• Analyzers integrated (< time-lags)• All analyzers temperature-controlled• Calibration automated • Analog signal transfer eliminated• System is indefinitely expandable to future

sensors, e.g. bicycle W, treadmill speed/elevation, IR activity, accelerometry, body weight, heart rate, body mass, food intake, etc. etc.

PROMETHION (room)

• No complex pressure etc. regulators required• Normocapnic, not steno/hypercapnic• High resolution analyzers plus background

baselining = higher flow rate (> 80 L/min)• Leak problems minimized• Single manufacturer = easy, single-point

support + FUD-less, quick setup!• Use for rooms, hoods or masks

PROMETHION (room)

PROMETHION• Push-button easy!• Raw data stored at 1 Hz• Any analytical protocol

can be applied• Drill down from processed

data to raw data• Users can share analytical

scripts

PROMETHION• Additional sensors are in

development• All sensors are sampled at

1 Hz in perfect synchrony with gas exchange data

• Opens the door to many new questions

• Need a feature? Ask!

PROMETHION

New Horizons

• Data Rate Changes from a trickle to a fire hose• Use as much as you need• Fully modular – add features as you need them• Automates analysis without losing flexibility and traceability• Sable Systems is committed to your success!

METABOLIC SCREENING

Questions?

THANK YOU!