sable’s scope who we are founded by john r.b. lighton, ph.d. 25 years of scientific discovery...
TRANSCRIPT
SABLE’S SCOPEWho We Are
• Founded by John R.B. Lighton, Ph.D.• 25 years of scientific discovery• Tools for understanding – with a respirometry focus• Scientists and engineers still active in research• Growing at 25% for last 4 years• Growing at over 45% in 2011• Fueled by Promethion
SABLE’S GLOBAL INFLUENCE
Sable’s Promethion InspirationCUSTOMER REQUESTS:
Turnkey Solution
Fully Integrated
Live-in Cages with Bedding
Fully Traceable Data
Running Wheel
Expertise
High Throughput
Scalable
Accurate
User Friendly
No air tight seal
Good Customer Service
Customizable Analysis
No Chemical Desiccants
MARKET RESEARCH: Competition
• Black box calculations• No raw data storage or data traceability• Customer service• Difficult to use• Very complex to set up
The Good
• Widely used and cited• Integrated system• High Throughput
The Bad
• Small, not live-in cages• No bedding in cages• Very low flow rates (IACUC)• Accuracy concerns• No integrated running wheels
The Ugly
METABOLIC SCREENING
EXISTING CUSTOMERS
THE CAGE…
FOOD INTAKE
Food Intake Monitor
Features
• 3mg resolution• Crumb tray reduces caching• Real-time measurement
• Universal Load Cell• Mouse or Rat hopper
Advantages
• Increased accuracy, versatility• Fine granularity gives insight into behavioral patterns
FOOD INTAKERaw Feeding Data
• Stores hopper mass vs. time• Recorded to disc at 1Hz
One Feeding Episode
• Analyze behavior• Determine individual feeding episode intake rates
FOOD INTAKESummarized Feeding Data
• Identify trends and outliers• Can zoom into any event• Automated uptake analysis• Automated meal analysis
One Feeding Episode
• Analyze behavior• Unprecedented detail available – but only if required
FOOD INTAKEProcessed Feeding Data
• Outputs cumulative intake vs. time• Shows rate of intake, structure• Can output binned data• Integrates with other cage data• Fully traceable
Food Selection
• Determine food preference• Control access to either/both foods• Integrates with other cage data
FOOD INTAKE ANALYSIS
FOOD ACCESS CONTROL
Access Control Module
Features
• Computer controlled access to food• Available for Mouse or Rat food hoppers• Intelligent Obstruction Detection
• Access Control Door• Connects to mass sensor
Advantages
• Reduced technician interaction • Customizable assays (paired/yoked)
FOOD ACCESS CONTROLUser Friendly Setup
• Select from 8 preset assays• Customize time and duration• Individual cage setup
Select Access Control Type
• Add to your custom assay• Unlimited number of variations
WATER INTAKE
Water Intake Monitor
Features
• 3 µL resolution• Real-time measurement• Stainless steel case (not shown) reduces animal interaction• Custom sipper tube reduces leakage
• Universal Load Cell• Mouse or Rat dispenser models
Advantages
• Increased accuracy• Increased granularity gives insight into behavioral patterns• Use existing sterilizing systems
WATER INTAKERaw Drinking Data
• Stores bottle mass vs. time• Recorded to disc at 1Hz
Processed Drinking Data
• Analyze behavior• Mathematical drift elimination
• Determine individual episode intake rates• EthoScan Compatible
BODY MASS
Body Mass Monitor
Features
• 3mg resolution• Real-time measurement
• Universal load cell• Mouse or Rat models available
Advantages
• Highly accurate• Provides in-cage enrichment• Reduced technician interaction
BODY MASSRaw Body Mass Data
• Stores bracket mass vs. time• Recorded to disc at 1Hz• Is used about every 15 minutes
Zoomed In
• Track body mass over time
BODY MASSProcessed Body Mass Data
• Stores most recent body mass for each timestamp• Synchronized with other data
• Diurnal cycle clearly visible
VOLUNTARY EXERCISE
Wheel-M or Wheel-R
Features
• Real-time measurement• Stainless steel construction• Entirely in cage
• Mouse or Rat Wheel• Magnetic Reed Switch• Optional: Wheel Stop
Advantages
• Increased granularity gives insight into behavioral patterns• Correlates with metabolic data
VOLUNTARY EXERCISERaw Running Wheel Data
• Stores RPS vs. time• Recorded to disc at 1Hz
Processed Running Wheel Data
• Shows cumulative distance run• Can be binned and synchronized with metabolic data
TOTAL ACTIVITY
BXYZ-M or BXYZ-R
• XY and Z IR arrays• Mounting bracket
Features
• 0.25cm calculated centroid• Real-time measurement• Intelligent obstruction detection• Rearing captured with Z axis
Advantages
• Highly accurate• Not affected by static obstructions• Correlated with metabolic data
TOTAL ACTIVITYActivity Analysis
• Total Activity• Total Distance Traveled• Rearing• Activity associated with in-cage devices• In cage position vs. time• Customizable assays• Position histograms
Activity Comparison
• WT vs. ob/ob• Interaction with FH-MB• Time spent time spent near food hopper
CALORIMETRY
Flow Generators & Gas Analyzers
• Automated calibration • Sequential or continuous monitoring• Can group for 4, 8,16, 24, 32 etc. cages• Expandable and modular• Water vapor dilution correction• Integrated O2, CO2, WVP analyzers
• Pull-mode (negative pressure)
DATA ANALYSIS Data Synchronization
• Overlay metabolic data with other continuously monitored parameters
Further Analysis
• All data are perfectly synchronized and can be exported to other programs if required (open formats)
DATA ANALYSIS Further Data Synchronization
•Body mass and food intake
Advantages
• Synchronized data yields traceability• Real time measurement improves visibility• Both enable behavioral monitoring
•Body mass and water intake
•Body mass, food intake, water intake, and wheel revolutions
DATA ANALYSIS Multiplexed vs. Continuous
• Continuous measurements give unprecedented detail• Multiplexed measurements are adequate for many research topics• You have the choice
TOTAL ACTIVITYActivity Analysis (II)
• Activity within user-defined areas of any shape• Regions of interest interactively defined on customizable background• Correlate with other data streams as needed
TOTAL ACTIVITYActivity Analysis (III)
TOTAL ACTIVITYActivity Analysis (IV)
DATA ANALYSIS
DATA ANALYSIS
DATA ANALYSIS
Multiplexing errors- more information
Error from multiplexing
Over and underestimates
DATA ANALYSISRaw Data Synchronization - Zoomed
• Body mass (blk), food intake (red), wheel (yel), and water intake (blu)
ETHOSCANBehavioral Monitoring
• All cage devices continuously monitored (“focal animal”)• Interactions sequentially processed in data analysis• Output is timestamp, position, duration, amount and activity
ETHOSCAN
Behavioral Transition Matrix
• Statistical analysis of interaction with in-cage devices• Shows probability of behaviors in the top row happening immediately after the behavior in the leftmost column
ETHOSCANTime Budgets
• Shows percentage of time spent exhibiting a specific behavior• Customizable to your cage setup and behaviors you wish to monitor
Ambulatory Budgets
• Two wild type mice –with and without access to a wheel• Note the significant difference in distance travelled
ROOM CALORIMETRY
ROOM CALORIMETRY• Time-consuming to set up and test• Just one span per run… But analyzers drift• Propane burn validations may require back-calculation of
span gases (black box!)• Cannot adequately account for water vapor dilution,
especially at low WVP• System integration difficult• Many individual instruments = many failure points and
many companies to deal with• Little or no expandability or modularity
ROOM CALORIMETRY
ROOM CALORIMETRY• Raw data not preserved, so - Not auditable• Comparisons between systems problematic• Little emphasis on traceability (pre-Jon Moon)• But – traceability alone will not help• Also need transparency!• Custom “black box” software is proprietary• Software is a priori optimized for
distinguishing between two activities
ROOM CALORIMETRY• Highly stable O2, CO2 analyzers required
• Low noise (for response correction)• WV dilution is a problem…• Chemicals are impractical…• Typically, chilled condensers are used• But these only “dry” the air to ~650 Pa WVP• At dewpoints < 1 C, they have no effect at all• Plus, FiCO2 changes…
ROOM CALORIMETRY
FiCO2 changes…
ROOM CALORIMETRY +
• Minimize O2 drift (SSI O2 analyzers)
• Then, eliminate O2 drift by repeated, interleaved FiO2 measurement (PP)
• For CO2, use interleaved SSI analyzers (PP)
• For eliminating WV dilution, don’t scrub/condense – calculate from WVP, BP! (PP)
• Record all raw data in complete detail
ROOM CALORIMETRY +• Use computational processing to reassemble
the dry O2 and CO2 data from the interleaved O2, CO2, WVP data, plus BP (PP)
• Apply rigorously neutral smoothing and response correction to data
• All using a traceable, auditable, shareable script
• Because raw data are stored, any kind of response correction you wish can be applied!
PROMETHION
PROMETHION
• Analyzers integrated (< time-lags)• All analyzers temperature-controlled• Calibration automated • Analog signal transfer eliminated• System is indefinitely expandable to future
sensors, e.g. bicycle W, treadmill speed/elevation, IR activity, accelerometry, body weight, heart rate, body mass, food intake, etc. etc.
PROMETHION (room)
• No complex pressure etc. regulators required• Normocapnic, not steno/hypercapnic• High resolution analyzers plus background
baselining = higher flow rate (> 80 L/min)• Leak problems minimized• Single manufacturer = easy, single-point
support + FUD-less, quick setup!• Use for rooms, hoods or masks
PROMETHION (room)
PROMETHION• Push-button easy!• Raw data stored at 1 Hz• Any analytical protocol
can be applied• Drill down from processed
data to raw data• Users can share analytical
scripts
PROMETHION• Additional sensors are in
development• All sensors are sampled at
1 Hz in perfect synchrony with gas exchange data
• Opens the door to many new questions
• Need a feature? Ask!
PROMETHION
New Horizons
• Data Rate Changes from a trickle to a fire hose• Use as much as you need• Fully modular – add features as you need them• Automates analysis without losing flexibility and traceability• Sable Systems is committed to your success!
METABOLIC SCREENING
Questions?
THANK YOU!