sat type fmd outbreaks in south africa · 2012. 6. 14. · sat type fmd outbreaks in south africa...
Post on 25-Jan-2021
0 Views
Preview:
TRANSCRIPT
-
SAT TYPE FMD OUTBREAKS IN
SOUTH AFRICA
Dwarka RMSemenya D, Tshabalala T, Mtshali N, Esterhuysen J,
Botha B, Kirkbride E, Jones H, Mkhwanazi R, Shileyi B, Mkhantshwa C, Mutowembwa P.
Trans-boundary Animal Diseases Programme
-
Range of tests for FMD
(OIE/FAO Reference laboratory status)
• Liquid phase blocking ELISA
• Solid phase competition ELISA
• Virus neutralisation
• Virus isolation
• Non-structural protein ELISA
• Typing (antigen) ELISA
• Molecular diagnostics: PCR, sequencing, phylogenetic analysis of outbreak strains
• Disinfectant testing
-
Route of possible outbreak samples
Clinical samples
Seq and
Phylogenetics
PCRVirus isolation
Ground Clinical samples
Epidemiological PCRDiagnostic PCR
REPORT
-
FMD CONTROL ZONES
-
SWAZILAND
MOZAMBIQUE
BOTSWANA
MBABANE MAPUTO
JOHANNESBURG
PRETORIA
PIETERSBURG
GABORONE
KRUGERNATIONAL
PARK
SOUTH AFRICA
LESOTHO
PIETERMARITZBURG
BUSHBUCKRIDGE
NKOMAZIMIDDELBURG
CAMPERDOWN
DURBAN
BEIT BRIDGE
ZIMBABWE
MASISI AREA
KNP/4/02/ElandskuilKNP/5/02/Hlamalala
SAR/01/02/Phalaborwa-gateKNP/10/90/Reenvoeldam
KNP/1/03/Lower-SabieKNP/2/03/Letaba
SAR/14/01/MessinaZIM/Gn39/91ZIM/3/94
KNP/8/02/MasakosapanKNP/08/03/LangtoondamKNP/9/96
KNP/2/97KNP/1/02/NwarihlangariSAR/2/06/Sibasa/Matiane Diptank
SAR/4/06/Sibasa/Joseph DiptankSAR/1/06/Sibasa/Matiane DiptankSAR/3/06/Sibasa/Matiane Diptank
KNP/6/88ZIM/Hv5/90
KNP/33/94KNP/44/94
KNP/15/96NAM/274/98ZIM/32/90
ZIM/5/91ZIM/05/91ZAM/5/93
ZAM/4/96
100
100
100
99
92
99
100
100
8099
90
95
92
0.02
SAT 3: Sept 2006: SibasaThis outbreak occurred well within the OIE recognised buffer zone of the FMD control zone of SA adjacent to the KNP. The status of South Africa's "FMD free zone without
vaccination" has not been affected by this outbreak, and therefore South Africa's FMD status has not changed and the export of animals and animal products from South Africa is not affected (OIE report).
-
June 2008: SAT 3 Impala: PafuriSAT 3 isolate clustered as
part of the southern topotype
of viruses, but differed by
16% from the SAT 3
outbreak strain characterised
from the Sibasa area in 2006
KNP/5/02/HlamalalaKNP/4/06/Crooks Corner
SAR/01/02/Phalaborwa-gateKNP/25/94/Lower-SabieKNP/1/03/Lower-Sabie
KNP/21/08/Lower SabieKNP/16/08/Lower Sabie
KNP/19/08/Lower SabieKNP/2/03/Letaba
10/1337/NDUMO BUFFALOKNP/3/08/Lower SabieKNP/4/10/Lalapalm Windmill
KNP/5/08/Lower SabieZIM/Gn9/91
ZIM/Ck4/91ZIM/3/94SAR/14/01/Messina
KNP/6/88KNP/8/02/MasakosapanKNP/1/08/Impala
KNP/26/07/BoyelaKNP/2/07/DzomboZIM/3/10/Sengwe Corridor
KNP/1/02/NwarihlangariSAR/1/06/Sibasa/Matiane DiptankKNP/08/03/LangtoondamKNP/11/96
KNP/2/97KNP/3/94KNP/44/94ZIM/2/10/Sengwe CorridorKNP/6/08/Lower Sabie
ZIM/Hv5/90ZIM/5/91ZIM/05/91
ZIM/24/91ZAM/7/93
ZAM/4/96ZAM/11/96
99
9999
99
99
99
99
7199
99
91
74
99
79
80
91
95
65
98
79
82
84
-
Jan 2011:SAT 2: ImpalaMOZ/1/10/Gaza ProvinceKNP/1/10/Crooks Corner
KNP/9/03/Ribye-WaterholeKNP/1/06/2/Masakosa
KNP/9/08/Lower SabieKNP/2/10/Banyini PanKNP/3/10/Banyini PanKNP/25/07/Boyela
KNP/160/91KNP/5/06/2/Nwambi blockKNP/4/07/Dzombo
KNP/16/93/CapricornKNP/183/91/Krokodilbrugarea
KNP/05/03/Lower-SabieKNP/6/96KNP/1678/98/BumeKNP/06/03/Shangoni
KNP/11/07/BububuKNP/16/07/ShingumeniKNP/15/07/ShingumeniKNP/18/07/ShingumeniKNP/20/07/Langtoon Dam
SAR/2/04/Selwana-farmSAR/01/01/Mhala-districtKNP/12/08/Lower Sabie
KNP/07/88KNP/19/89KNP/18/95KNP/1/11/Impala
SAR/1801/1/Spelenyane DTSAR/1801/2/Spelenyane DTSAR/1801/3/Spelenyane DTSAR/1801/4/Spelenyane DTKNP/04/03/Punda-Maria
SAR/1/10/Limpopo Safaris/buffaloKNP/143/91KNP/32/92/Boyela
KNP/5/91/SataraSAR/1/03/Masisi
SAR/1/08/Maruleng/buffaloZIM/6/03/HarareZIM/7/03/ChinhoyiZIM/9/03/HarareBOT/31/98/Vumbura
NAM/1/08/NamibiaSWA/4/89
ZIM/09/02/BikitaZIM/10/02/BikitaZIM/01/01/Bulawayo
BOT/13/02BOT/4/06/2BOT/5/06/2100
10094
99
58100
98
8583
100
100
100
99
263547
100
99
98
100
99
57
86
99
77
65
87
62
97
85
80
61
67
79
53
SAT 2 strain characterised
from impala at
Renosterkoppies, KNP
differs by 6% from the SAT 2
outbreak strain
-
Outbreaks in MP Protection zone
(Dr Ben du Plessis)
• SAT 1: 2009
• SAT 2: 2011/2012
-
FMD: Kwa-Zulu Natal
FMD
outbreak
-
First serological positive sera
(February 2011)
-
POS1.51.4
-
Red dots: positive LPBE
Yellow: negative LPBE
-
PCR on probangs/clinical samples
from Ndumo, Ezulwini• 7/25 weak PCR positive
• No virus cultured in PK cells
Lesion material: Zidlele DT• No Positives on PCR
• No virus isolated on PK cells
Buffalo sampling (Ndumo Game
Reserve)
• Sera and probangs from buffalo
-
Buffalo:
Serology: High SAT 3
Ab titres
NSP positive
Initial Outbreak
area
-
KNP/4/02/Elandskuil
KNP/5/02/Hlamalala
KNP/4/06/Crooks Corner
SAR/01/02/Phalaborwa-gate
KNP/25/94/Lower-Sabie
KNP/10/90/ReenvoeldamKNP/2/03/Letaba
10/1337/NDUMO BUFFALOKNP/1/03/Lower-Sabie
KNP/8/02/Masakosapan
KNP/1/08/Impala
ZIM/Gn9/91
ZIM/Ck4/91
ZIM/Ck10/91
SAR/14/01/Messina
ZIM/Gn39/91
ZIM/3/94
ZIM/Hv5/90
KNP/26/07/Boyela
KNP/2/07/Dzombo
KNP/08/03/Langtoondam
KNP/9/96
KNP/11/96
KNP/3/97
KNP/2/97KNP/1/02/Nwarihlangari
SAR/2/06/Sibasa/Matiane Diptank
SAR/4/06/Sibasa/Joseph Diptank
SAR/1/06/Sibasa/Matiane Diptank
SAR/3/06/Sibasa/Matiane Diptank
KNP/33/94
KNP/14/96
KNP/15/96
KNP/3/94
KNP/44/94
KNP/6/88
ZIM/32/90
NAM/10/1054/buffalo
ZIM/11/94
BOT/9/98
NAM/274/98
ZAM/5/93
ZAM/7/93
ZAM/4/96
ZAM/11/96
ZIM/31/90
ZIM/24/91
ZIM/16/91
ZIM/5/91
ZIM/05/91
100
100100
99
100
94
92
100
100
71
86
100
99
99
93
100
9491
100
99
83
93
79
99
80
76
98
89
90
88
0
.
KZN: Ndumo
Nature Reserve
SAT 3: buffalo
-
Positive Serological results in feedlot in Gauteng
-
Virus isolation: KZN samples
• VI was a challenge using PK cells
• ZZR2 goat cell lines and the BHK
cells (Kerstin and Juanita)
• ZZR2 BHK2 cattle (caused
lesions)
• Sequenced lesion material
-
Pictures of lesions observed in
experimentally infected cattle (KZN
SAT 1 strain)
Courtesy of Dr Paidamwoyo Mutowembwa
-
KNP/35/92/SHINGWEDZIKNP/36/92/SHINGWEDZI
KNP/29/92/PAFURIKNP/10/07/Bububu
KNP/29/07/MpongoloKNP/9/07/DzombieneKNP/3/07/DzomboKNP/5/07/DzomboKNP/8/07/Dzombiene
KNP/8/95/MONDSWENIKNP/14/95/MONDSWENI
KNP/7/03/ShangoniKNP/45/92/SHINGWEDZI
KNP/1584/98/Baranukee10/1364/Zidlele DT KZN epithelium10/1693/Feedlot GP lymph node
KNP/6/06/MakwadsiKNP/03/03/Pafuri
KNP/886/98/SHIPANDI-WINDMILLKNP/893/98/SHIPANDI-WINDMILL
KNP/10/03/NshawuKNP/3/02/Elandskuil
KNP/2/02/ElandskuilKNP/2/06/Maseya Sandveld
KNP/3/06/Maseya SandveldGN13/91KNP/01/01/Shipandi-Windmill
SAR/9/81MOZ/03/77
KNP/24/91/NTSHAWAKNP/1/05
SAR/1/09/Makoko DTSAR/5/09/Phameni DT
KNP/5/05KNP/196/91KNP/148/91
HV3/90ZIM/05/99
KNP/11/03/MasoriniKNP/21/07/Langtoon Dam
SAR/08/02/MauricedaleKNP/17/96
SWL/01/00SAR/32/00KNP/397/98/RIETPANKNP/400/98/RIETPAN
KNP20/89/KWA-MFAMEBTOSAR/1/00/Buffalo/PhalaborwaSAR/7/03/buffaloSAR/6/03/buffalo
10/939/Welverdien buffalo probang10/507/Phalaborwa buffalo10/501/Gravelotte Cattle epithelium10/587/Gravelotte cattle epithelium10/547/Gravelotte Cattle probang
BOT/2/98BOT/8/98
SWA/2/89BOT/1/06/1
NAM/307/98NAM/306/98NAM/308/98
BOT/37/98ZIM/02/03/Chiredzi
ZIM/3/88ZAM/1/05
ZAM/06/1ZAM/29/96ZAM/2/93TAN/01/99
MAL/1/85ZIM/03/95ZIM/14/98
ZIM/9A/91ZIM/08/03/BulawayoZIM/11/03MOZ/03/02MOZ/01/02MOZ/02/02
99
99
99
99
99
99
99
99
70
99
75
99
94
99
52
99
99
99
99
99
99
99
97
99
86
99
99
99
91
92
84
58
77
60
79
96
5779
57
51
0.02
Southern
Topotype
Western
Topotype
Northern
Topotype
KZN: SAT 1
-
Surveillance:
Jan 2012
• 800 sera randomly selected
• 50 diptanks: 16 sera per diptank
-
Acknowledgement
• Dr Grietjie De Klerk (DAFF) for maps
-
Thank You
top related